Author(s): Portuondo, Deivys Leandro [UNESP] ; Dores-Silva, Paulo Roberto ; Ferreira, Lucas Souza [UNESP] ; de Oliveira, Carlos S. ; Téllez-Martínez, Damiana [UNESP] ; Marcos, Caroline Maria [UNESP] ; de Aguiar Loesch, Maria Luiza [UNESP] ; Guzmán, Fanny ; Gava, Lisandra M. ; Borges, Júlio César ; Pereira, Sandro Antonio ; Batista-Duharte, Alexander [UNESP] ; Carlos, Iracilda Zeppone [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199694
Origin: Oasisbr
Made available in DSpace on 2020-12-12T01:46:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01
In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of S. schenckii cell wall proteins. Here, we sought to assess the protective potential of a Sporothrix spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the S. brasiliensis infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with S. brasiliensis as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after in vitro stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both S. schenckii and S. brasiliensis. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis.
São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis
São Carlos Institute of Chemistry University of São Paulo, P.O. Box 780
Núcleo Biotecnológico de Curauma (NBC) Pontificia Universidad Católica de Valparaíso
Center of Biological and Health Sciences Federal University of São Carlos
Oswaldo Cruz Foundation (Fiocruz) Evandro Chagas National Institute of Infectious Diseases (INI) Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals (Lapclin-Dermzoo)
São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis
