Author(s): Santos, Catiule de Oliveira ; Costa, Sidnei Ferro [UNESP] ; Souza, Fabiana Santana ; Mendes, Jessica Mariane Ferreira ; Pinheiro, Cristiane Garboggini Melo de ; Moreira, Diogo Rodrigo de Magalhães ; Silva, Luciano Kalabric ; Lima, Valeria Marçal Felix de [UNESP] ; Oliveira, Geraldo Gileno de Sá
Date: 2021
Persistent ID: http://hdl.handle.net/11449/208362
Origin: Oasisbr
Made available in DSpace on 2021-06-25T11:10:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01
rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum.
Oswaldo Cruz Foundation Gonçalo Moniz Research Center Laboratory of Structural and MolecularPathology (LAPEM) Tissue Engineering and Immunopharmacology Laboratory (LETI) or Pathology andMolecular Biology Laboratory (LPBM)
Department of Clinical Medicine Surgeryand Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine
Department of Clinical Medicine Surgeryand Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine
