Author(s): Morao, Luana G. [UNESP] ; Lorenzoni, Andre S. G. ; Chakraborty, Parichita ; Ayusso, Gabriela M. [UNESP] ; Cavalca, Lucia B. ; Santos, Mariana B. [UNESP] ; Marques, Beatriz C. [UNESP] ; Dilarri, Guilherme [UNESP] ; Zamuner, Caio [UNESP] ; Regasini, Luis O. [UNESP] ; Ferreira, Henrique [UNESP] ; Scheffers, Dirk-Jan
Date: 2021
Persistent ID: http://hdl.handle.net/11449/209614
Origin: Oasisbr
Subject(s): chalcone; Asiatic citrus canker; Bacillus subtilis; Xanthomonas citri
Made available in DSpace on 2021-06-25T12:23:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Xanthomonas citri subsp. citri (X. citri) is an important phytopathogen and causes Asiatic Citrus Canker (ACC). To control ACC, copper sprays are commonly used. As copper is an environmentally damaging heavy metal, new antimicrobials are needed to combat citrus canker. Here, we explored the antimicrobial activity of chalcones, specifically the methoxychalcone BC1 and the hydroxychalcone T9A, against X. citri and the model organism Bacillus subtilis. BC1 and T9A prevented growth of X. citri and B. subtilis in concentrations varying from 20 mu g/mL to 40 mu g/mL. BC1 and T9A decreased incorporation of radiolabeled precursors of DNA, RNA, protein, and peptidoglycan in X. citri and B. subtilis. Both compounds mildly affected respiratory activity in X. citri, but T9A strongly decreased respiratory activity in B. subtilis. In line with that finding, intracellular ATP decreased strongly in B. subtilis upon T9A treatment, whereas BC1 increased intracellular ATP. In X. citri, both compounds resulted in a decrease in intracellular ATP. Cell division seems not to be affected in X. citri, and, although in B. subtilis the formation of FtsZ-rings is affected, a FtsZ GTPase activity assay suggests that this is an indirect effect. The chalcones studied here represent a sustainable alternative to copper for the control of ACC, and further studies are ongoing to elucidate their precise modes of action.
Univ Estadual Paulista, Dept Bioquim & Microbiol, Inst Biociencias, BR-13050690 Rio Claro, SP, Brazil
Univ Groningen, Dept Mol Microbiol, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen, Netherlands
Univ Estadual Paulista, Dept Quim & Ciencias Ambientais, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
Univ Estadual Paulista, Dept Bioquim & Microbiol, Inst Biociencias, BR-13050690 Rio Claro, SP, Brazil
Univ Estadual Paulista, Dept Quim & Ciencias Ambientais, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
Nederlandse Organisatie voor Wetenschappelijk Onderzoek: 729.004.005
FAPESP: 2017/50216-0 ; 2014/18330-0; 2018/15083-2; 2014/18577-5; 2016/08084-7; 2014/18719-4; 2016/07108-0
CNPq: 246986/2013-1; 471129/2013-5; 306251/2016-7; 429322/2018-6
