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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 Spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpoles/5 replicates of 10 animals) from species Physalaemus cuvieri (Leptodactylidae). After synthesis, purification, and characterization of peptides (PSDP2001, PSDP2002, PSDP2003) an aquatic contamination has been simulated with these peptides during 24 h of exposure in two concentrations (100 and 500 ng/mL). The control group (“C”) was composed of tadpoles kept in polyethylene containers containing de-chlorinated water. Oxidative stress, antioxidant biomarkers and AChE activity were assessed. In both concentrations, PSPD2002 and PSPD2003 increased catalase and superoxide dismutase antioxidants enzymes activities, as well as oxidative stress (nitrite levels, hydrogen peroxide and reactive oxygen species). All three peptides also increased acetylcholinesterase activity in the highest concentration. These peptides showed molecular interactions in silico with acetylcholinesterase and antioxidant enzymes. Aquatic particle contamination of SARS-CoV-2 has cholinesterasic effect in P. cuvieri tadpoles. These findings indicate that the COVID-19 can constitute environmental impact or biological damage potential.
Department of Pharmacology Institute of Biomedical Sciences University of Sao Paulo
Post-graduation Program in Biotechnology and Biodiversity Goiano Federal Institution and Federal University of Goiás
Biological Research Laboratory Post-graduation Program in Conservation of Cerrado Natural Resources Goiano Federal Institute – Urata Campus
Center of Research in Inflammatory Diseases Ribeirão Preto Medical School University of São Paulo
Postgraduate Program in Morphological Science Department of Morphology School of Medicine Federal University of Ceara, Delmiro de Farias St.
Department of Immunology Institute of Biomedical Sciences University of Sao Paulo
Department of Physiology and Pharmacology School of Medicine Federal University of Ceara, Coronel Nunes de Melo St., 1127
Drug Research and Development Center Federal University of Ceara, Coronel Nunes de Melo St., 1000
Laboratory of Animal Pharmacology and Toxicology Brazil University
Department of Preventive Veterinary Medicine São Paulo State University (UNESP)
Laboratório de Controle Genético e Sanitário Diretoria Técnica de Apoio ao Ensino e Pesquisa Faculdade de Medicina da Universidade de São Paulo
Reproductive and Molecular Biology Group Institute of Biosciences São Paulo State University
Institute of Motricity Sciences Federal University of Alfenas
Faculty of Biosciences and Aquaculture Nord University
Department of Cell Biology Institute of Biomedical Sciences University of São Paulo
Institute of Chemistry São Paulo State University (UNESP)
Brigham and Women's Hospital Harvard Medical School, 75 Francis St
Department of Preventive Veterinary Medicine São Paulo State University (UNESP)
Reproductive and Molecular Biology Group Institute of Biosciences São Paulo State University
Institute of Chemistry São Paulo State University (UNESP)
CNPq: 307743/2018-7
CNPq: 426531/2018-3
FAPESP: FAPESP 2020/05761-3
