Author(s):
Mendonça-Gomes, Juliana Moreira ; Charlie-Silva, Ives ; Guimarães, Abraão Tiago Batista ; Estrela, Fernanda Neves ; Calmon, Marilia Freitas [UNESP] ; Miceli, Rafael Nava ; Sanches, Paulo R.S. [UNESP] ; Bittar, Cíntia [UNESP] ; Rahal, Paula [UNESP] ; Cilli, Eduardo M. [UNESP] ; Ahmed, Mohamed Ahmed Ibrahim ; Vogel, Christoph F.A. ; Malafaia, Guilherme
Date: 2022
Persistent ID: http://hdl.handle.net/11449/231484
Origin: Oasisbr
Subject(s): Contamination; Developmental toxicity; Insects; Peptides; SARS-CoV-2; Water
Description
Made available in DSpace on 2022-04-29T08:45:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-15
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Instituto Federal Goiás
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Knowledge about how the COVID-19 pandemic can affect aquatic wildlife is still extremely limited, and no effect of SARS-CoV-2 or its structural constituents on invertebrate models has been reported so far. Thus, we investigated the presence of the 2019-new coronavirus in different urban wastewater samples and, later, evaluated the behavioral and biochemical effects of the exposure of Culex quinquefasciatus larvae to two SARS-CoV-2 spike protein peptides (PSPD-2002 and PSPD-2003) synthesized in our laboratory. Initially, our results show the contamination of wastewater by the new coronavirus, via RT-qPCR on the viral N1 gene. On the other hand, our study shows that short-term exposure (48 h) to a low concentration (40 μg/L) of the synthesized peptides induced changes in the locomotor and the olfactory-driven behavior of the C. quinquefascitus larvae, which were associated with increased production of ROS and AChE activity (cholinesterase effect). To our knowledge, this is the first study that reports the indirect effects of the COVID-19 pandemic on the larval phase of a freshwater invertebrate species. The results raise concerns at the ecological level where the observed biological effects may lead to drastic consequences.
Departamento de Imunologia Instituto de Ciências Biomédicas Universidade de São Paulo
Departamento de Farmacologia Instituto de Ciências Biomédicas Universidade de São Paulo
Programa de Pós-Graduação Em Conservação de Recursos Naturais do Cerrado Instituto Federal Goiano
Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho”
SeMAE Serviço Municipal Autonômo de Água e Esgoto São José do Rio Preto
Instituto de Química Universidade Estadual Paulista
Plant Protection Department Faculty of Agriculture Assiut University
Department of Environmental Toxicology and Center for Health and the Environment University of California
Programa de Pós-Graduação Em Biotecnologia e Biodiversidade Universidade Federal de Goiás
Programa de Pós-Graduação Em Ecologia e Conservação de Recursos Naturais Universidade Federal de Uberlância
Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho”
Instituto de Química Universidade Estadual Paulista
FAPESP: 19/19939–1
FAPESP: 2020/05761–3
Instituto Federal Goiás: 23219.000340.2021-13
CNPq: 307743/2018–7
CNPq: 426531/2018–3
