Autor(es): Conte, Fernanda Lopes [UNESP] ; Pereira, Ana Catarina ; Brites, Gonçalo ; Ferreira, Isabel ; Silva, Ana Cristina ; Sebastião, Ana Isabel ; Matos, Patrícia ; Pereira, Cláudia ; Batista, Maria Teresa ; Sforcin, José Maurício [UNESP] ; Cruz, Maria Teresa
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/234217
Origem: Oasisbr
Assunto(s): Anti-inflammatory action; Antiallergic action; Antioxidant activity; Monocyte; Propolis
Made available in DSpace on 2022-05-01T15:13:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-05-01
Background: Propolis is a bee product used since ancient times due to its diverse biological applications. We aimed to investigate the antioxidant and anti-inflammatory action of Brazilian propolis in THP-1 cells stimulated with 1‐fluoro‐2,4‐dinitrobenzene (DNFB) or lipopolysaccharide (LPS). Methods: Cell viability was assessed by resazurin assay, antioxidant activity was evaluated by DPPH assay, superoxide dismutase (SOD) 1 and 2 activity was evaluated by colorimetric assay. CD86 expression was determined by flow cytometry. IL-1β and HMOX-1 were analysed by Western blot. Results: Propolis did not affect cell viability and exhibited a potent antioxidant activity. Propolis alone induced SOD 1 activity. LPS stimulated SOD2, concomitantly or not with propolis. In the presence of LPS, propolis induced a higher HMOX-1 expression. Propolis inhibited CD86 expression stimulated by DNFB. LPS induced pro-IL-1β expression and propolis did not affect its action. Conclusion: Propolis exhibited an antioxidant action in a cell-free system and in a cell based-model. Propolis exerted an anti-inflammatory/antiallergic action, probably due to its antioxidant activity.
Institute of Biosciences Department of Chemical and Biological Sciences São Paulo State University (UNESP)
Center for Neuroscience and Cell Biology (CNC)
Faculty of Pharmacy Pólo das Ciências da Saúde University of Coimbra
University of Coimbra CIEPQPF FFUC Pólo das Ciências da Saúde, Azinhaga de Santa Comba
LAQV REQUIMTE Faculty of Pharmacy University of Coimbra, Azinhaga de Santa Comba
Institute of Biosciences Department of Chemical and Biological Sciences São Paulo State University (UNESP)
