Author(s):
Ribeiro, Artur J. ; Volkov, Vadim ; Oliveira, Mariana Braga ; Padrão, Jorge ; Mano, J. F. ; Gomes, Andreia C ; Cavaco-Paulo, Artur
Date: 2016
Persistent ID: https://hdl.handle.net/1822/41808
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876/147337/PT;
info:eu-repo/grantAgreement/FCT/5876/147364/PT;
Subject(s): Tunable scaffolds; Hydrophobic/Hydrophilic ratio; Induced cold gelation; Surface wettability; Mechanical properties; Cell proliferation; Science & Technology
Description
"Available online 6 May 2016"
An effective tissue engineering approach requires adjustment according to the target tissue to be engineered. The possibility of obtaining a protein-based formulation for the development of multivalent tunable scaffolds that can be adapted for several types of cells and tissues is explored in this work. The incremental substitution of bovine serum albumin (BSA) by human serum albumin (HSA), changing the scaffolds hydrophilic/hydrophobic ratio, on a previously optimized scaffold formulation resulted in a set of uniform porous scaffolds with different physical properties and associated cell proliferation profile along time. There was a general trend towards an increase in hydrophilicity, swelling degree and in vitro degradation of the scaffolds with increasing replacement of BSA by HAS. The set of BSA/HSA scaffolds presented distinct values for the storage (elastic) modulus and loss factor which were similar to those described for different native tissues such as bone, cartilage, muscle, skin and neural tissue. The preferential adhesion and proliferation of skin fibroblasts on the BSA25%HSA75% and HSA100% scaffolds, as predicted by their viscoelastic properties, demonstrate that the BSA/HSA scaffold formulation is promising for the development of scaffolds that can be tuned according to the tissue to be repaired and restored.
This work was supported by FCT I.P through the strategic funding UID/BIO/04469/2013 and the strategic funding UID/BIA/04050/2013. Artur Ribeiro thanks FCT for the SFRHBPD983882013 grant.
