Author(s):
Pedrosa, Sílvia S. ; Pereira, Paula ; Correia, A. ; Gama, F. M.
Date: 2017
Persistent ID: https://hdl.handle.net/1822/47800
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876/147337/PT;
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT
;
info:eu-repo/grantAgreement/FCT/COMPETE/126270/PT;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61516%2F2009/PT;
Subject(s): Hyaluronic acid; Cancer nanotechnology; Non-invasive imaging; Non-small cancer lung cells; Science & Technology
Description
We have, in previous work developed, characterized and evaluated the biocompatibility of an engineered hyaluronic acid nanogel. Here we assess the targetability of a hyaluronic acid nanogel towards CD44 overexpressing cells, in vitro and in vivo. Results obtained by flow cytometry and confocal fluorescence microscopy shows that nanogel is greatly internalized by non-small cancer lung cells (A549 cells), that overexpress CD44 receptors. The biodistribution and tumor targetability of the nanogel labelled with a near-infrared (NIR) probe were performed, in mice, through a non-invasive imaging system. Results revealed nanogel high targetability towards an induced subcutaneous A549 tumor. Nanogels pharmacokinetics was evaluated also in healthy animals, and Alexa Fluor 680 labelled nanogel exhibited higher accumulation in liver, kidneys and skin. Also, a comparative biodistribution study was performed, using two NIR imaging probes, Cy5.5 and Alexa Fluor 680.
The authors thank the FCT Strategic Project of UID/BIO/04469/ 2013 unit, the project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124FEDER-027462) and the Project “BioHealth - Biotechnology and Bioengineering approaches to improve health quality”, Ref. NORTE07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDER. We would like to acknowledge also the support of FCT for the PhD grant reference SFRH/BD/61516/2009. We would also like to thank Bioimaging department on Molecular Medicine Institute (IMM) in Lisbon, namely Dr. José Rino and Dr. António Temudo. Also thank the animal facilities in IMM (Lisbon), specially Dra. Dolores Bonaparte and Dra. Joana Marques. Also, we would like to acknowledge the special contribution of Professor Fatima Baltazar in ICVS (Braga) for kindly grant us CFBE and A549 cell lines.
info:eu-repo/semantics/publishedVersion
