Author(s):
Francisco, Miguel ; Aguiar, Tatiana Quinta ; Abreu, Gabriel ; Marques, Susana ; Gírio, Francisco ; Domingues, Lucília
Date: 2023
Persistent ID: https://hdl.handle.net/1822/86406
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT;
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBII-BTI%2F1858%2F2021/PT;
Subject(s): Ashbya gossypii; optimization; lipid production; bio-jet fuel; by-product valorization; biorefineries
Description
In this work, microbial lipid production from non-detoxified Eucalyptus bark hydrolysate (EBH) with oleaginous xylose-utilizing Ashbya gossypii strains was explored. The best producing strain from a set of engineered strains was identified in synthetic media mimicking the composition of the non-detoxified EBH (SM), the lipid profile was characterized, and yeast extract and corn steep liquor (CSL) were pinpointed as supplements enabling a good balance between lipid accumulation, biomass production, and autolysis by A. gossypii. The potential of the engineered A. gossypii A877 strain to produce lipids was further validated and optimized with minimally processed inhibitor-containing hydrolysate and high sugar concentration, and scaled up in a 2 L bioreactor. Lipid production from non-detoxified EBH supplemented with CSL reached a lipid titer of 1.42 g/L, paving the way for sustainable single-cell oil production within the concept of circular economy and placing lipids as an alternative by-product within microbial biorefineries.
This study was supported by Compete 2020, Portugal 2020, and Lisboa 2020 through MoveToLowC (POCI-01-0247-FEDER-046117) and by the Portuguese Foundation for Science and Technology (FCT) through the strategic funding of UIDB/04469/2020 and project ESSEntial (PTDC/BII-BTI/1858/2021). The authors gratefully acknowledge RAIZ (Forest and Paper Research Institute) for providing the Eucalyptus bark material, and Novozymes A/S for supplying Cellic® CTec3 HS. The technical assistance of STEX company (Aveiro, Portugal) on the operation of pilot-scale infrastructure of steam explosion and enzymatic hydrolysis is also acknowledged.
info:eu-repo/semantics/publishedVersion
