Author(s): Strepis, Nikolaos ; Sánchez-Andrea, Irene ; van Gelder, Antonie H. ; van Kruistum, Henri ; Shapiro, Nicole ; Kyrpides, Nikos ; Göker, Markus ; Klenk, Hans-Peter ; Schaap, Peter J. ; Stams, Alfons Johannes Maria ; Sousa, Diana Zita Machado
Date: 2016
Persistent ID: https://hdl.handle.net/1822/47806
Origin: RepositóriUM - Universidade do Minho
Subject(s): Citrate; DDH; Fermentation; Genome blast distance phylogeny; Genomics; Trichococcus
Species of the genus Trichococcus share high similarity of their 16S rRNA gene sequences (>99%). Digital DNADNA hybridization values (dDDH) among type strains of all described species of the genus Trichococcus ( T. flocculiformis DSM 2094T , T. pasteurii DSM 2381T , T. collinsii DSM 14526T , T. palustris DSM 9172T, and T. patagoniensis DSM 18806T) indicated that Trichococcus sp. strain R210T represents a novel species of the genus Trichococcus . The dDDH values showed a low DNA relatedness between strain R210T and all other species of the genus Trichococcus (2332%). Cells of strain R210T were motile, slightly curved rods, 0.631.40×0.480.90 µm and stained Gram-positive. Growth was optimal at pH7.8 and at temperature of 30°C. Strain R210T could utilize several carbohydrates, and the main products from glucose fermentation were lactate, acetate, formate and ethanol. The genomic DNA G+C content of strain R210T was 47.9mol%. Based on morphological, physiological and biochemical characteristics along with measured dDDH values for all species of the genus Trichococcus , it is suggested that strain R210T represents a novel species within the genus Trichococcus , for which the name Trichococcus ilyis sp. nov. is proposed. The type strain is R210T (=DSM 22150T=JCM 31247T).
