Author(s): Magalhães, F. F. ; Alves, Carolina ; Pedro, A. Q. ; Freire, M. G. ; Tavares, A. P.
Date: 2023
Persistent ID: http://hdl.handle.net/10773/43224
Origin: RIA - Repositório Institucional da Universidade de Aveiro
Subject(s): laccase; recombinant production; aqueous biphasic systems; extractive fermentation
Extractive fermentation is an integrated process that uses aqueous biphasic systems to combine the production and primary purification procedures into a single step. With this concept, the main goal of this work was to design a bioprocess that integrates laccase production and purification using a genetically engineered yeast (Pichia pastoris), creating a competitive and sustainable process. Successful expression in P. pastoris resulted in the production of extracellular recombinant laccase. Microbial growth and laccase production in fermentation was evaluated in media with ABS constituents to integrate the purification step. The laccase produced was recovered, concentrated by precipitation, and characterised. This work demonstrates that ABS is a promising alternative for integrative protein production platforms, with the potential to minimise production steps and, consequently, process costs.
