Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®

• Autor(es): de Miranda, Silvana Spindola ; de Almeida, Isabela Neves ; de Fátima Filardi Oliveira Mansur, Maria ; de Assis Figueredo, Lida Jouca ; da Silva Carvalho, Wânia ; Hadaad, João Paulo Amaral ; de Lourdes do Carmo Guimarães Diniz, Jaciara ; von Groll, Andrea ; da Silva, Pedro Almeida ; Lopes, Maria Luiza ; dos Santos, Marcelo Cordeiro ; Brito, Alexandra ; de Queiroz Mello, Fernanda Carvalho ; da Silva Santos Malaquias, Thiago ; Croda, Julio ; Pinhata, Juliana Maira Watanabe ; de Oliveira, Rosângela Siqueira ; Chimara, Erica ; Rossetti, Maria Lúcia ; Halon, Maria Laura ; Lourenço, Maria Cristina ; de Melo Medeiros, Reginalda Ferreira ; Montes, Fátima Cristina Onofre Fandinho ; Machado, Diana ; Viveiros, Miguel ; Kritski, Afrânio Lineu
• Data: 2020
• Identificador Persistente: http://hdl.handle.net/10362/116609
• Origem: Repositório Institucional da UNL
• Assunto(s): MDR; Nitrate reductase; Technology; Tuberculosis; Biochemistry, Genetics and Molecular Biology (miscellaneous); Infectious Diseases; Microbiology; SDG 3 - Good Health and Well-being
• Tipo de Documento: Artigo científico
• Idioma: Inglês
• Contribuidor(es): Instituto de Higiene e Medicina Tropical (IHMT); Global Health and Tropical Medicine (GHTM); TB, HIV and opportunistic diseases and pathogens (THOP); RUN

Descrição

(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.