Autor(es):
Mora, Blanca de Juan ; Filipe, Luís ; Forte, Andreia ; Santos, Miguel M. ; Alves, Celso ; Teodoro, Fernando ; Pedrosa, Rui ; Carrott, Manuela Ribeiro ; Branco, Luís C. ; Gago, Sandra
Data: 2021
Identificador Persistente: http://hdl.handle.net/10362/142688
Origem: Repositório Institucional da UNL
Projeto/bolsa:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT;
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI-QOR%2F32406%2F2017/PT;
Assunto(s): Antimicrobial activity; Ciprofloxacin; Cytotoxicity; Ionic liquids; Mesoporous silica nanoparticles; Pharmaceutical Science
Descrição
UID/MAR/04292/2020 PEst-C/LA0006/2013 RECI/BBBBQB/0230/2012 MAR-02.01.01-FEAMP-0042–INOVA4AQUA 022161
Mesoporous silica nanoparticles (MSNs) are very promising nanomaterials for treating bacterial infections when combined with pharmaceutical drugs. Herein, we report the preparation of two nanomaterials based on the immobilization of ciprofloxacin in mesoporous silica nanoparticles, either as the counter-ion of the choline derivative cation (MSN-[Ch][Cip]) or via anchoring on the surface of amino-group modified MSNs via an amide bond (MSN-Cip). Both nanomaterials were characterized by TEM, FTIR and solution1H NMR spectroscopies, elemental analysis, XRD and N2 adsorption at 77 K in order to provide the desired structures. No cytotoxicity from the prepared mesoporous nanoparticles on 3T3 murine fibroblasts was observed. The antimicrobial activity of the nanomaterials was determined against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Klebsiella pneumoniae) bacteria and the results were promising against S. aureus. In the case of B. subtilis, both nanomaterials exhibited higher antimicrobial activity than the precursor [Ch][Cip], and in the case of K. pneumoniae they exhibited higher activity than neutral ciprofloxacin.
