Autor(es):
Lança, Miguel ; Gaio, Vânia ; Rodrigues, Ana Paula ; Henriques, Camila ; Gomes, Licínia ; Dias, Daniela ; Chasqueira, Maria-Jesus ; Guiomar, Raquel ; Melo, Aryse
Data: 2025
Identificador Persistente: http://hdl.handle.net/10362/189588
Origem: Repositório Institucional da UNL
Assunto(s): children; Europe; molecular characterization; respiratory viruses; RSV; surveillance; Epidemiology; Pulmonary and Respiratory Medicine; Public Health, Environmental and Occupational Health; Infectious Diseases; SDG 3 - Good Health and Well-being
Descrição
Funding Information: Funding: The authors received no specific funding for this work. We gratefully acknowledge all data contributors, authors and their originating laboratories and the contribution of all physicians that collaborated in the VigiRSV network (VigiRSV network: Teresa Gouveia, Regina Viseu, Maria Zarcos, Ricardo Castro, Jorge Rodrigues, Virg\u00EDnia Martinez, Eurico Jorge Gaspar, Carlos Caldas, Eliana Costa, Susana Castanhinha, Rita C\u00F4rte-Real, Miguel F\u00E9lix, Lurdes Correia, Arminda Jorge, Patr\u00EDcia Ibarzabal, Daniela Couto, Ros\u00E1rio Barreto, Carolina Constant, Teresa Bandeira, Carla Cruz, Ana Paquete, Elsa Rocha, Ana Loureiro, Sid\u00F3nia Nunes, Jos\u00E9 Alves, Teresa Nunes, Teresa Gra\u00E7a, In\u00EAs Azevedo, Sara Catarino, Teresa Almeida, Gra\u00E7a Ferreira, Luciana Barbosa, Isabel Carvalho, V\u00E2nia Soares, Paulo Lopes, Rita Marques and Raquel Marques) and the Sentinel Influenza and other respiratory viruses surveillance network. Publisher Copyright: © 2025 The Author(s). Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
Background: Respiratory syncytial virus (RSV) is the leading cause of acute respiratory infection (ARI) in young children, but its genetic diversity requires ongoing surveillance. Methods: From 2021 to 2023, a total of 619 and 94 RSV-positive samples from the National Respiratory Syncytial Virus Surveillance Network (VigiRSV) and the Sentinel Influenza and other respiratory viruses surveillance network (Sentinel ISN), respectively, were analysed. The RSV A and RSV B typing was assessed by a multiplex real-time RT-PCR. Sanger sequencing was performed on a subset of samples (n = 495). Phylogenetic analysis was carried out on partial glycoprotein G sequences. Clinical and epidemiological data were compared through Pearson Chi-Square tests. Results: RSV Subgroup A was more prevalent (53.5%, 85/159) in the 2021/2022 season, whereas in the 2022/2023 season, it was RSV Subgroup B (82.1%, 435/530) in both networks. RSV A strains in VigiRSV clustered mainly to A.D.1 (39.0%, 39/100), whereas in Sentinel ISN, they clustered in A.D.5 (30.0%, 3/10). RSV Type B clustered mainly to B.D.E.1 (96.6%, 372/385) in both networks. All lineages cocirculated during the study period and in both surveillance networks. Regional clusters were identified for both subgroups. Conclusions: This study provides new insights into RSV genetic variability in Portugal, namely, the cocirculation of lineages and intravariability among lineages within both subgroups during the study period and in all Portuguese regions. However, our study is based on partial sequencing of the G gene, and because of this limitation, our results should be considered with great caution.
