Direct PCR detection of foot-and-mouth disease virus

• Author(s): Rodriguez, A. ; Nunez, J. ; Nolasco, Gustavo ; Ponz, F. ; Sobrino, F. ; Deblas, C.
• Date: 1994
• Persistent ID: http://hdl.handle.net/10400.1/6228
• Origin: Sapientia - Universidade do Algarve
• Document Type: Journal article
• Language: English
• Contributor(s): Sapientia

Description

A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were achieved for viral samples of serotypes A, O and C, either from cell culture supernatants or from lesions of infected animals. The assay allowed detection of around 15 PFU, being 500-fold more sensitive than a conventional indirect ELISA. This new method constitutes a simple, rapid and efficient alternative for the diagnosis and characterization of FMDV by PCR.