Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

• Autor(es): von Groll,Andrea ; Martin,Anandi ; Portaels,Françoise ; Silva,Pedro Eduardo Almeida da ; Palomino,Juan Carlos
• Data: 2010
• Origem: Oasisbr
• Assunto(s): Growth kinetics; Mycobacterium tuberculosis; Resazurin; Fitness
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.