Author(s): Martins, Natália ; Barros, Lillian ; Carvalho, Ana Maria ; Silva, Sónia ; Henriques, Mariana ; Ferreira, Isabel C.F.R.
Date: 2014
Persistent ID: http://hdl.handle.net/10198/12330
Origin: Biblioteca Digital do IPB
Project/scholarship: info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F87658%2F2012/PT;
Opportunistic fungal infections have deserved special relevance in the last decades, presenting itself, a serious problem in terms of public health. Despite Candida albicans was considered the main agent responsible for those infections, other non-albicans Candida species have also been described in the last years [1-3]. Most of the species are susceptible to antimicrobial drugs, but recently it has been observed a growing number of microorganisms with drug resistance. Therefore, the discover/use of alternative therapies is crucial [4]. Juglans regia L. (walnut) leaves are commonly used in traditional medicine as antiseptic, antimicrobial and anti-inflammatory [5]; those benefits could be related with its richness in phenolic compounds [6]. In the present work, the antifungal potential of the hydroalcoholic extract prepared from walnut leaves was evaluated against a total of nineteen Candida strains (from the species: C.albicans, C.glabrata, C.parapsilosis and C.tropicalis), using the disc diffusion halo assay. All the tested strains were sensible to the plant extract. The obtained values of the inhibitory zones ranged between 0.9-1.4 cm, being the halo maintained after 48h. The observed antifungal activity is certainly related to the phenolic compounds previously determined in the extract [6]: five phenolic acid derivativescaffeoylquinic and p-coumaroylquinic acid derivatives, two dimers and one trimer of procyanidins, twelve flavonols- quercetin, myricetin and kaempferol derivatives, and five taxifolin O-pentoside isomers; 3-Ocaffeoylquinic acids and quercetin O-pentoside were the main phenolic compounds. Further studies are necessaries in order to elucidate the most active compounds and the specific role of each one.
