Author(s): Pires, Diana Priscila Penso ; Melo, Luís D. R.
Date: 2018
Persistent ID: http://hdl.handle.net/1822/56502
Origin: RepositóriUM - Universidade do Minho
Project/scholarship: info:eu-repo/grantAgreement/FCT/5876/147337/PT; info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT ; info:eu-repo/grantAgreement/FCT/COMPETE/126270/PT;
Subject(s): Phage infection; Planktonic cultures; Biofilm Flow cytometry; Biofilm; Flow cytometry
The in vitro activity of bacteriophages against planktonic cultures and biofilms is commonly evaluated by culture methods. However, these methods can lead to an underestimation of total bacterial cells when they undergo different physiological states. This chapter describes the methodology used to assess the in vitro activity of bacteriophages against planktonic cultures of bacteria in different metabolic states and biofilm populations by flow cytometry.
This work was supported by the Portuguese Foundation for Scienceand Technology(FCT)under thescope ofthe project PTDC/ BBB-BSS/6471/2014, the strategic funding of UID/BIO/ 04469/2013 unit and COMPETE 2020 (POCI-01-0145FEDER-006684), and under the scope of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). This work was also supported by BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020— Programa Operacional Regional do Norte.
info:eu-repo/semantics/publishedVersion
