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Medicinal plants are recognized since ancient times as therapeutic agents with several beneficiai properties for human health. Herein, the phenolic profile of three formulations (infusions, pills and syrups) based on artichoke, milk thistle and borututu was analysed as well as their bioactivity, namely the antioxidant, hepatoprotective and antimicrobial activities. In what concems the phenolic composition, the studied plants proved to represent a good source of bioactive compounds, especially phenolic acids and flavonoids. Regarding artichoke and milk thistle, phenolic compounds were higher in the infusion preparations when compared to the other formulations, but in the case of borututu, the syrup revealed the highest contents. Luteolin-7-0- glucuronide was the major flavonoid found in artichoke infusion, protocatechuic acid was the main constituent of borututu infusion, and isorhamnetin-0-deoxyhexoside-O-hexoside was the most abundant compound in milk thistle syrup.