Author(s):
Silva, Bárbara Roque da [UNESP] ; Cirelli, Thamiris [UNESP] ; Nepomuceno, Rafael [UNESP] ; Theodoro, Letícia Helena [UNESP] ; Orrico, Silvana R.P. [UNESP] ; Cirelli, Joni A. [UNESP] ; Barros, Silvana P. ; Scarel-Caminaga, Raquel M. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200997
Origin: Oasisbr
Subject(s): Diabetes mellitus; Genetic polymorphisms; Haplotypes; Interleukin; Periodontitis; Diabetes mellitus; Diabetes mellitus; Genetic polymorphisms; Genetic polymorphisms; Haplotypes; Haplotypes; Interleukin; Interleukin; Periodontitis; Periodontitis
Description
Made available in DSpace on 2020-12-12T02:21:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Background and aim: Type 2 Diabetes Mellitus (T2DM) and Periodontitis (P) are prevalent multifactorial disorders worldwide, sharing a bidirectional relationship influenced by the genetic susceptibility of the host immune system. We investigated whether SNPs in the Interleukin 8 (IL8, alias CXCL8) gene could be associated with T2DM and Periodontitis. Methods: Genomic DNA was obtained from 874 Brazilian individuals divided into: Healthy group (n = 307), Periodontitis group (n = 334), and individuals affected by both T2DM and Periodontitis (T2DM_P) group (n = 233). The SNPs −251(T>A) rs4073, +396(T>G) rs2227307 and +781(C>T) rs2227306 were genotyped by TaqMan®. Multiple logistic regressions were used to determine the degree of association between polymorphisms (and haplotypes) with periodontitis and T2DM adjusted for known confounders. Results: The additive model revealed that the heterozygous AT(-251), GT(+396) and CT(+781) genotypes showed a lower risk for the diseased phenotypes, and carriers of the TTC/TTC haplotype were significantly susceptible to T2DM and Periodontitis concomitantly, as well to isolated Periodontitis (mainly the severe form). Conclusions: We concluded, for the first time, that these functional CXCL8 SNPs, and the homozygous TTC haplotype are relevant genetic factors for T2DM and Periodontitis as comorbidities, as well as for severe Periodontitis susceptibility in Brazilian population.
Department of Diagnosis and Surgery São Paulo State University – UNESP School of Dentistry at Araraquara
Department of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University – UNESP School of Dentistry at Araraquara
Department of Surgery and Integrated Clinic São Paulo State University – UNESP School of Dentistry at Araçatuba
Advanced Research Center in Medicine Union of the Colleges of the Great Lakes (UNILAGO)
Department of Comprehensive Oral Health - Periodontology University of North Carolina at Chapel Hill – UNC School of Dentistry
Department of Diagnosis and Surgery São Paulo State University – UNESP School of Dentistry at Araraquara
Department of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University – UNESP School of Dentistry at Araraquara
Department of Surgery and Integrated Clinic São Paulo State University – UNESP School of Dentistry at Araçatuba
FAPESP: 2014/13295-1
FAPESP: 2016/03753-8
FAPESP: 2016/08070-6
FAPESP: 2016/18313-3
FAPESP: 2016/25418-6
FAPESP: 2018/26367-1
CNPq: 304570/2017-6
CNPq: 445336/2014-5
