Author(s):
Cury, Sarah Santiloni [UNESP] ; Lapa, Rainer Marco Lopez [UNESP] ; de Mello, Julia Bette Homem ; Marchi, Fábio Albuquerque ; Domingues, Maria Aparecida Custódio [UNESP] ; Pinto, Clóvis Antonio Lopes ; Carvalho, Robson Francisco [UNESP] ; de Carvalho, Genival Barbosa ; Kowalski, Luiz Paulo ; Rogatto, Silvia Regina
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201558
Origin: Oasisbr
Subject(s): Biomarker; Laryngeal squamous cell carcinoma; Liquid biopsy; Secretome; Transcriptome; Biomarker; Biomarker; Laryngeal squamous cell carcinoma; Laryngeal squamous cell carcinoma; Liquid biopsy; Liquid biopsy; Secretome; Secretome; Transcriptome; Transcriptome
Description
Made available in DSpace on 2020-12-12T02:35:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Uddannelses- og Forskningsministeriet
Objectives: The tumor secretome deconvolution is a promising strategy to identify diagnostic and prognostic biomarkers. Here, transcriptomic-based secretome analysis was performed aiming to discover laryngeal squamous cell carcinomas (LSCC) biomarkers from potentially secreted proteins (PSPs). Material and Methods: The tumor expression profile (35 LSCC biopsies compared with surrounding normal tissues - SN) revealed 589 overexpressed genes. This gene list was used for secretome analysis based on laryngeal tumors and related secretome databases. Results: Forty-nine (Laryngeal tumor secretome database) and 50 (Human Protein Atlas and Cancer Secretome Database) PSPs presented an association with worse overall survival. Specifically, DSG2 overexpression was strongly correlated with poor survival and distant metastasis. DSG2 increased expression was confirmed in the LSCC dataset (LSCC = 111; SN = 12) from TCGA. A significant association between shorter survival and DSG2 overexpression was also detected. In an independent cohort of cases, we analyzed and confirmed high protein levels of DSG2 in plasma from LSCC patients. Conclusion: A set of PSPs including the circulating DSG2, were associated with shorter overall survival in LSCC. DSG2 overexpression was also correlated with distant metastasis. The high plasmatic protein levels of DSG2 suggest its potential to be tested in liquid biopsies and applied as prognostic biomarker of LSCC.
Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
Department of Head and Neck Surgery and Otorhinolaryngology A.C. Camargo Cancer Center
International Research Center CIPE – A.C. Camargo Cancer Center
Department of Pathology Faculty of Medicine São Paulo State University (UNESP)
Department of Pathology A.C. Camargo Cancer Center
Department of Clinical Genetics University Hospital Institute of Regional Health Research University of Southern Denmark
Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
Department of Pathology Faculty of Medicine São Paulo State University (UNESP)
