Author(s):
Rinaldo, Daniel [UNESP] ; Silva, Marcelo Aparecido [UNESP] ; Rodrigues, Clenilson Martins [UNESP] ; Calvo, Tamara Regina [UNESP] ; Sannomiya, Miriam [UNESP] ; Santos, Lourdes Campaner dos [UNESP] ; Vilegas, Wagner [UNESP] ; Kushima, Hélio [UNESP] ; Hiruma-Lima, Clélia Akiko [UNESP] ; Brito, Alba Regina Monteiro de Souza
Date: 2014
Persistent ID: http://hdl.handle.net/11449/26375
Origin: Oasisbr
Subject(s): HSCCC; Davilla elliptica; flavonoids; HSCCC; HSCCC; Davilla elliptica; Davilla elliptica; flavonoids; flavonoids
Description
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses.
Universidade Estadual Paulista Instituto de Química Departamento de Química Orgânica
Universidade Estadual Paulista Instituto de Biociências Departamento de Fisiologia
Universidade Estadual de Campinas Instituto de Biologia Departamento de Fisiologia e Biofísica
Universidade Estadual Paulista Instituto de Química Departamento de Química Orgânica
Universidade Estadual Paulista Instituto de Biociências Departamento de Fisiologia
