Publicação
Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
| Resumo: | An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells. |
|---|---|
| Autores principais: | Jiang, Hua |
| Outros Autores: | Yi, Jun; Boavida, Leonor C.; Chen, Yuan; Becker, Jörg D.; Köhler, Claudia; McCormick, Sheila |
| Assunto: | pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| Idioma: | inglês |
| Origem: | ARCA |
| _version_ | 1867149333049114624 |
|---|---|
| author | Jiang, Hua |
| author2 | Yi, Jun Boavida, Leonor C. Chen, Yuan Becker, Jörg D. Köhler, Claudia McCormick, Sheila |
| author2_role | author author author author author author |
| author_facet | Jiang, Hua Yi, Jun Boavida, Leonor C. Chen, Yuan Becker, Jörg D. Köhler, Claudia McCormick, Sheila |
| author_role | author |
| contributor_name_str_mv | ARCA |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Jiang, Hua\"},{\"Person.name\":\"Yi, Jun\"},{\"Person.name\":\"Boavida, Leonor C.\"},{\"Person.name\":\"Chen, Yuan\"},{\"Person.name\":\"Becker, Jörg D.\"},{\"Person.name\":\"Köhler, Claudia\"},{\"Person.name\":\"McCormick, Sheila\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | ARCA |
| datacite.creators.creator.creatorName.fl_str_mv | Jiang, Hua Yi, Jun Boavida, Leonor C. Chen, Yuan Becker, Jörg D. Köhler, Claudia McCormick, Sheila |
| datacite.date.Accepted.fl_str_mv | 2015-10-27T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-11-18T15:21:14Z |
| datacite.date.embargoed.fl_str_mv | 2015-11-18T15:21:14Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus |
| datacite.titles.title.fl_str_mv | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| dc.contributor.none.fl_str_mv | ARCA |
| dc.creator.none.fl_str_mv | Jiang, Hua Yi, Jun Boavida, Leonor C. Chen, Yuan Becker, Jörg D. Köhler, Claudia McCormick, Sheila |
| dc.date.Accepted.fl_str_mv | 2015-10-27T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-11-18T15:21:14Z |
| dc.date.embargoed.fl_str_mv | 2015-11-18T15:21:14Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.7/500 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | National Academy of Sciences |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus |
| dc.title.fl_str_mv | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://arca.gimm.pt/bitstreams/abf4bf06-aaf0-4568-9103-ef127f87d1e6/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | EXPL/BIA-PLA/0244/2013 |
| funding.name_str_mv | 3599-PPCDT |
| funding_str_mv | EXPL/BIA-PLA/0244/2013 info:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-PLA%2F0244%2F2013/PT |
| id | arca_3c56e4e3f25b9e4c8a19faab4170e7be |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.7/500 |
| instacron_str | arca |
| institution | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| instname_str | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| language | eng |
| network_acronym_str | arca |
| network_name_str | ARCA |
| oai_identifier_str | oai:arca.gimm.pt:10400.7/500 |
| organization_str_mv | urn:organizationAcronym:arca |
| person_str_mv | Jiang, Hua Yi, Jun Boavida, Leonor C. Chen, Yuan Becker, Jörg D. Köhler, Claudia McCormick, Sheila |
| publishDate | 2015 |
| publisher.none.fl_str_mv | National Academy of Sciences |
| reponame_str | ARCA |
| repository_id_str | urn:repositoryAcronym:arca |
| service_str_mv | urn:repositoryAcronym:arca |
| spelling | engNational Academy of Sciencespt_PTAn Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.application/pdfpt_PTIntercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to spermJiang, HuaYi, JunBoavida, Leonor C.Chen, YuanBecker, Jörg D.Köhler, ClaudiaMcCormick, SheilaHostingInstitutionOrganizationalARCAe-mailmailto:arca.repository@gimm.ptarca.repository@gimm.ptDOIIsPartOf10.1073/pnas.15108541122015-11-18T15:21:14Z2015-10-272015-10-27T00:00:00ZHandlehttp://hdl.handle.net/10400.7/500http://purl.org/coar/access_right/c_abf2open accesspollenspermmRNA transportprotein phosphatase 2Cvegetative nucleus1123910 bytesFundação para a Ciência e a TecnologiaThe other Side of the Mirror: mRNA Transport and Non-Cell-Autonomous Activity in the Male Germ Unitinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-PLA%2F0244%2F2013/PTEXPL/BIA-PLA/0244/20133599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2015-10-27http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://arca.gimm.pt/bitstreams/abf4bf06-aaf0-4568-9103-ef127f87d1e6/downloadProceedings of the National Academy of Sciences of the United States of America112431337813383 |
| spellingShingle | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm Jiang, Hua pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus |
| status | SINGLETON |
| subject.fl_str_mv | pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus |
| title | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| title_full | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| title_fullStr | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| title_full_unstemmed | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| title_short | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| title_sort | Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm |
| topic | pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus |
| topic_facet | pollen sperm mRNA transport protein phosphatase 2C vegetative nucleus |
| url | http://hdl.handle.net/10400.7/500 |
| visible | 1 |
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