Publicação
Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses
| Resumo: | Glutamate receptors are well characterized channels that mediate cell-to-cell communication during neurotransmission in animals. Nevertheless, information regarding their functional role in organisms without nervous systems is still limited. In plants, Glutamate Receptor-like (GLR) genes have been implicated in defence against pathogens, reproduction, control of stomata aperture and light signal transduction(1-5). However, the numerous GLR genes present in angiosperm genomes (20 to 70)(6) has prevented the observation of strong phenotypes in loss-of-function mutants. Here, we show that in the moss Physcomitrella patens, a basal land plant, mutation of GLR genes cause sperm failure in targeting the female reproductive organs. In addition, we show that GLR genes encode non-selective Ca(2+) permeable channels that can regulate cytoplasmic Ca(2+) and are needed to induce the expression of a BELL1-like transcription factor essential for zygote development. Our work reveals novel functions for GLRs in sperm chemotaxis and transcriptional regulation. Sperm chemotaxis is essential for fertilization in both animals and early land plants like bryophytes and pteridophytes. Therefore, our results are suggestive that ionotropic glutamate receptors may have been conserved throughout plant evolution to mediate cell-to-cell communication during sexual reproduction. |
|---|---|
| Autores principais: | Ortiz-Ramírez, Carlos |
| Outros Autores: | Michard, Erwan; Simon, Alexander A.; Damineli, Daniel S. C.; Hernández-Coronado, Marcela; Becker, Jörg D.; Feijó, José A. |
| Assunto: | Fertilization Evolutionary developmental biology |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| Idioma: | inglês |
| Origem: | ARCA |
| _version_ | 1868974515129679872 |
|---|---|
| author | Ortiz-Ramírez, Carlos |
| author2 | Michard, Erwan Simon, Alexander A. Damineli, Daniel S. C. Hernández-Coronado, Marcela Becker, Jörg D. Feijó, José A. |
| author2_role | author author author author author author |
| author_facet | Ortiz-Ramírez, Carlos Michard, Erwan Simon, Alexander A. Damineli, Daniel S. C. Hernández-Coronado, Marcela Becker, Jörg D. Feijó, José A. |
| author_role | author |
| contributor_name_str_mv | ARCA |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ortiz-Ramírez, Carlos\"},{\"Person.name\":\"Michard, Erwan\"},{\"Person.name\":\"Simon, Alexander A.\"},{\"Person.name\":\"Damineli, Daniel S. C.\"},{\"Person.name\":\"Hernández-Coronado, Marcela\"},{\"Person.name\":\"Becker, Jörg D.\"},{\"Person.name\":\"Feijó, José A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | ARCA |
| datacite.creators.creator.creatorName.fl_str_mv | Ortiz-Ramírez, Carlos Michard, Erwan Simon, Alexander A. Damineli, Daniel S. C. Hernández-Coronado, Marcela Becker, Jörg D. Feijó, José A. |
| datacite.date.Accepted.fl_str_mv | 2017-07-24T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-07-01T00:30:08Z |
| datacite.date.embargoed.fl_str_mv | 2018-07-01T00:30:08Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Fertilization Evolutionary developmental biology |
| datacite.titles.title.fl_str_mv | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| dc.contributor.none.fl_str_mv | ARCA |
| dc.creator.none.fl_str_mv | Ortiz-Ramírez, Carlos Michard, Erwan Simon, Alexander A. Damineli, Daniel S. C. Hernández-Coronado, Marcela Becker, Jörg D. Feijó, José A. |
| dc.date.Accepted.fl_str_mv | 2017-07-24T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-07-01T00:30:08Z |
| dc.date.embargoed.fl_str_mv | 2018-07-01T00:30:08Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.7/780 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Nature Publishing Group |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Fertilization Evolutionary developmental biology |
| dc.title.fl_str_mv | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Glutamate receptors are well characterized channels that mediate cell-to-cell communication during neurotransmission in animals. Nevertheless, information regarding their functional role in organisms without nervous systems is still limited. In plants, Glutamate Receptor-like (GLR) genes have been implicated in defence against pathogens, reproduction, control of stomata aperture and light signal transduction(1-5). However, the numerous GLR genes present in angiosperm genomes (20 to 70)(6) has prevented the observation of strong phenotypes in loss-of-function mutants. Here, we show that in the moss Physcomitrella patens, a basal land plant, mutation of GLR genes cause sperm failure in targeting the female reproductive organs. In addition, we show that GLR genes encode non-selective Ca(2+) permeable channels that can regulate cytoplasmic Ca(2+) and are needed to induce the expression of a BELL1-like transcription factor essential for zygote development. Our work reveals novel functions for GLRs in sperm chemotaxis and transcriptional regulation. Sperm chemotaxis is essential for fertilization in both animals and early land plants like bryophytes and pteridophytes. Therefore, our results are suggestive that ionotropic glutamate receptors may have been conserved throughout plant evolution to mediate cell-to-cell communication during sexual reproduction. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://arca.gimm.pt/bitstreams/e000cafa-c447-4b2a-b06f-03f3d75c5cfb/download |
| id | arca_4f1d324560ff4ebcfa2af71b97fa7d7e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.7/780 |
| inst_facet_str | urn:organizationAcronym:arca{{{_:::_}}}Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| instacron_str | arca |
| institution | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| instname_str | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| language | eng |
| network_acronym_str | arca |
| network_name_str | ARCA |
| oai_identifier_str | oai:arca.gimm.pt:10400.7/780 |
| organization_str_mv | urn:organizationAcronym:arca |
| person_str_mv | Ortiz-Ramírez, Carlos Michard, Erwan Simon, Alexander A. Damineli, Daniel S. C. Hernández-Coronado, Marcela Becker, Jörg D. Feijó, José A. |
| publishDate | 2017 |
| publisher.none.fl_str_mv | Nature Publishing Group |
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| spelling | engNature Publishing Grouppt_PTGlutamate receptors are well characterized channels that mediate cell-to-cell communication during neurotransmission in animals. Nevertheless, information regarding their functional role in organisms without nervous systems is still limited. In plants, Glutamate Receptor-like (GLR) genes have been implicated in defence against pathogens, reproduction, control of stomata aperture and light signal transduction(1-5). However, the numerous GLR genes present in angiosperm genomes (20 to 70)(6) has prevented the observation of strong phenotypes in loss-of-function mutants. Here, we show that in the moss Physcomitrella patens, a basal land plant, mutation of GLR genes cause sperm failure in targeting the female reproductive organs. In addition, we show that GLR genes encode non-selective Ca(2+) permeable channels that can regulate cytoplasmic Ca(2+) and are needed to induce the expression of a BELL1-like transcription factor essential for zygote development. Our work reveals novel functions for GLRs in sperm chemotaxis and transcriptional regulation. Sperm chemotaxis is essential for fertilization in both animals and early land plants like bryophytes and pteridophytes. Therefore, our results are suggestive that ionotropic glutamate receptors may have been conserved throughout plant evolution to mediate cell-to-cell communication during sexual reproduction.application/pdfpt_PTGlutamate receptor-like channels are essential for chemotaxis and reproduction in mossesOrtiz-Ramírez, CarlosMichard, ErwanSimon, Alexander A.Damineli, Daniel S. C.Hernández-Coronado, MarcelaBecker, Jörg D.Feijó, José A.HostingInstitutionOrganizationalARCAe-mailmailto:arca.repository@gimm.ptarca.repository@gimm.ptDOIIsPartOf10.1038/nature234782018-07-01T00:30:08Z2017-07-242017-07-24T00:00:00ZHandlehttp://hdl.handle.net/10400.7/780http://purl.org/coar/access_right/c_abf2open accessFertilizationEvolutionary developmental biology13637560 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://arca.gimm.pt/bitstreams/e000cafa-c447-4b2a-b06f-03f3d75c5cfb/downloadNature124 |
| spellingShingle | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses Ortiz-Ramírez, Carlos Fertilization Evolutionary developmental biology |
| status | SINGLETON |
| subject.fl_str_mv | Fertilization Evolutionary developmental biology |
| title | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| title_full | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| title_fullStr | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| title_full_unstemmed | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| title_short | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| title_sort | Glutamate receptor-like channels are essential for chemotaxis and reproduction in mosses |
| topic | Fertilization Evolutionary developmental biology |
| topic_facet | Fertilization Evolutionary developmental biology |
| url | http://hdl.handle.net/10400.7/780 |
| visible | 1 |