Publicação
Diet leaves a genetic signature in a keystone member of the gut microbiota
| Resumo: | Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis). Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored. We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens. B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans. Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens. These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals. |
|---|---|
| Autores principais: | Dapa, Tanja |
| Outros Autores: | Ramiro, Ricardo Serotte; Pedro, Miguel Filipe; Gordo, Isabel; Xavier, Karina Bivar |
| Assunto: | microbiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| Idioma: | inglês |
| Origem: | ARCA |
| _version_ | 1867149333646802944 |
|---|---|
| author | Dapa, Tanja |
| author2 | Ramiro, Ricardo Serotte Pedro, Miguel Filipe Gordo, Isabel Xavier, Karina Bivar |
| author2_role | author author author author |
| author_facet | Dapa, Tanja Ramiro, Ricardo Serotte Pedro, Miguel Filipe Gordo, Isabel Xavier, Karina Bivar |
| author_role | author |
| contributor_name_str_mv | ARCA |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Dapa, Tanja\"},{\"Person.name\":\"Ramiro, Ricardo Serotte\"},{\"Person.name\":\"Pedro, Miguel Filipe\"},{\"Person.name\":\"Gordo, Isabel\"},{\"Person.name\":\"Xavier, Karina Bivar\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | ARCA |
| datacite.creators.creator.creatorName.fl_str_mv | Dapa, Tanja Ramiro, Ricardo Serotte Pedro, Miguel Filipe Gordo, Isabel Xavier, Karina Bivar |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-03-10T15:55:52Z |
| datacite.date.embargoed.fl_str_mv | 2022-03-10T15:55:52Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | microbiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses |
| datacite.titles.title.fl_str_mv | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| dc.contributor.none.fl_str_mv | ARCA |
| dc.creator.none.fl_str_mv | Dapa, Tanja Ramiro, Ricardo Serotte Pedro, Miguel Filipe Gordo, Isabel Xavier, Karina Bivar |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-03-10T15:55:52Z |
| dc.date.embargoed.fl_str_mv | 2022-03-10T15:55:52Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.7/976 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | microbiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses |
| dc.title.fl_str_mv | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis). Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored. We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens. B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans. Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens. These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://arca.gimm.pt/bitstreams/302e0ae2-1745-4708-b968-f9385b73635c/download |
| id | arca_fca47ba2a5c2cbccf8495c4dcf63cf24 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.7/976 |
| instacron_str | arca |
| institution | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| instname_str | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| language | eng |
| network_acronym_str | arca |
| network_name_str | ARCA |
| oai_identifier_str | oai:arca.gimm.pt:10400.7/976 |
| organization_str_mv | urn:organizationAcronym:arca |
| person_str_mv | Dapa, Tanja Ramiro, Ricardo Serotte Pedro, Miguel Filipe Gordo, Isabel Xavier, Karina Bivar |
| publishDate | 2022 |
| reponame_str | ARCA |
| repository_id_str | urn:repositoryAcronym:arca |
| service_str_mv | urn:repositoryAcronym:arca |
| spelling | engpt_PTSwitching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis). Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored. We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens. B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans. Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens. These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.application/pdfpt_PTDiet leaves a genetic signature in a keystone member of the gut microbiotaDapa, TanjaRamiro, Ricardo SerottePedro, Miguel FilipeGordo, IsabelXavier, Karina BivarHostingInstitutionOrganizationalARCAe-mailmailto:arca.repository@gimm.ptarca.repository@gimm.ptDOIIsPartOf10.1016/j.chom.2022.01.0022022-03-10T15:55:52Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.7/976http://purl.org/coar/access_right/c_abf2open accessmicrobiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses6085087 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://arca.gimm.pt/bitstreams/302e0ae2-1745-4708-b968-f9385b73635c/downloadCell Host & Microbe302183199.e10https://www.sciencedirect.com/science/article/pii/S1931312822000385?dgcid=author |
| spellingShingle | Diet leaves a genetic signature in a keystone member of the gut microbiota Dapa, Tanja microbiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses |
| status | SINGLETON |
| subject.fl_str_mv | microbiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses |
| title | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| title_full | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| title_fullStr | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| title_full_unstemmed | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| title_short | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| title_sort | Diet leaves a genetic signature in a keystone member of the gut microbiota |
| topic | microbiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses |
| topic_facet | microbiota, high-fat high-sugar diet, Western-style diet, microbiota evolution, gut dysbiosis, Bacteroides thetaiotaomicron, Bacteroidetes, gut metabolon, gut ecology, multi-omics analyses |
| url | http://hdl.handle.net/10400.7/976 |
| visible | 1 |