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Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds

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Resumo:The European hackberry (Celtis australis L.) is a deciduous tree of the Cannabaceae family frequently found in southern Europe and western Asia. Its fruit is an edible, dark-purple berry-like drupe with a large seed that corresponds to about 38% of the fruit dry weight [ l ]. Fruit preparations are used in traditional medicine as a remedy for colic, peptic ulcers, dysentery, diarrhea, amenorrhea, and heavy menstrual bleeding [1-3]. However, as far as we know, the antioxidant and antimicrobial properties of C. australis seeds have never been explored before, being this the purpose ofthis study. The C. australis seed extract was obtained by a solid-liquid extraction of the powdered sample with methanol water (80:20, v/v) [4]. Then, the antioxidant activity was measured in vitro via DPPH' scavenging activity, reducing power, p-carotene bleaching inhibition capacity, and thiobarbituric acid reactive substances (TBARS) formation inhibition capacity in brain cell homogenates [4]. The antimicrobial activity was screened against twelve microorganisms, including Gram-positive and Gram-negative bactéria and fungi, by the microdilution method [5]. The seed extract was effective in inhibiting the formation of TBARS as byproducts Of lipid peroxidation, which was concluded from the low EC^ value. The extract also had an interesting reducing power and capacity to protect p-carotene from the free radicais generated from linoleic acid. Regarding its antimicrobial activity, the best results were against B. cereus, with MIC and MBC lower than ampicillin, A. ochraceus, with MIC and MFC lower than ketoconazole, and P. funiculosum, with MIC lower than ketoconazole and bifonazole and MFC lower than ketoconazole. These results highlighted the potential of C. australis seeds as an interesting fruit byproduct to be used in the development of bio-based antimicrobials.
Autores principais:Alonso-Esteban, José Ignacio
Outros Autores:Pinela, José; Soković, Marina; Barros, Lillian; Sánchez-Mata, María Cortes; Torija-Isasa, Esperanza; Ferreira, Isabel C.F.R.
Assunto:European hackberry Celtis australís L.
Ano:2018
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:português
Origem:Biblioteca Digital do IPB
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author Alonso-Esteban, José Ignacio
author2 Pinela, José
Soković, Marina
Barros, Lillian
Sánchez-Mata, María Cortes
Torija-Isasa, Esperanza
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author
author_facet Alonso-Esteban, José Ignacio
Pinela, José
Soković, Marina
Barros, Lillian
Sánchez-Mata, María Cortes
Torija-Isasa, Esperanza
Ferreira, Isabel C.F.R.
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Alonso-Esteban, José Ignacio\"},{\"Person.name\":\"Pinela, José\",\"Person.identifier.orcid\":\"0000-0001-7523-1637\"},{\"Person.name\":\"Soković, Marina\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Sánchez-Mata, María Cortes\"},{\"Person.name\":\"Torija-Isasa, Esperanza\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Alonso-Esteban, José Ignacio
Pinela, José
Soković, Marina
Barros, Lillian
Sánchez-Mata, María Cortes
Torija-Isasa, Esperanza
Ferreira, Isabel C.F.R.
datacite.date.Accepted.fl_str_mv 2018-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2019-01-07T12:13:55Z
datacite.date.embargoed.fl_str_mv 2019-01-07T12:13:55Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv European hackberry
Celtis australís L.
datacite.titles.title.fl_str_mv Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Alonso-Esteban, José Ignacio
Pinela, José
Soković, Marina
Barros, Lillian
Sánchez-Mata, María Cortes
Torija-Isasa, Esperanza
Ferreira, Isabel C.F.R.
dc.date.Accepted.fl_str_mv 2018-01-01T00:00:00Z
dc.date.available.fl_str_mv 2019-01-07T12:13:55Z
dc.date.embargoed.fl_str_mv 2019-01-07T12:13:55Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/18311
dc.language.none.fl_str_mv por
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv European hackberry
Celtis australís L.
dc.title.fl_str_mv Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description The European hackberry (Celtis australis L.) is a deciduous tree of the Cannabaceae family frequently found in southern Europe and western Asia. Its fruit is an edible, dark-purple berry-like drupe with a large seed that corresponds to about 38% of the fruit dry weight [ l ]. Fruit preparations are used in traditional medicine as a remedy for colic, peptic ulcers, dysentery, diarrhea, amenorrhea, and heavy menstrual bleeding [1-3]. However, as far as we know, the antioxidant and antimicrobial properties of C. australis seeds have never been explored before, being this the purpose ofthis study. The C. australis seed extract was obtained by a solid-liquid extraction of the powdered sample with methanol water (80:20, v/v) [4]. Then, the antioxidant activity was measured in vitro via DPPH' scavenging activity, reducing power, p-carotene bleaching inhibition capacity, and thiobarbituric acid reactive substances (TBARS) formation inhibition capacity in brain cell homogenates [4]. The antimicrobial activity was screened against twelve microorganisms, including Gram-positive and Gram-negative bactéria and fungi, by the microdilution method [5]. The seed extract was effective in inhibiting the formation of TBARS as byproducts Of lipid peroxidation, which was concluded from the low EC^ value. The extract also had an interesting reducing power and capacity to protect p-carotene from the free radicais generated from linoleic acid. Regarding its antimicrobial activity, the best results were against B. cereus, with MIC and MBC lower than ampicillin, A. ochraceus, with MIC and MFC lower than ketoconazole, and P. funiculosum, with MIC lower than ketoconazole and bifonazole and MFC lower than ketoconazole. These results highlighted the potential of C. australis seeds as an interesting fruit byproduct to be used in the development of bio-based antimicrobials.
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oai_identifier_str oai:bibliotecadigital.ipb.pt:10198/18311
organization_str_mv urn:organizationAcronym:ipb
person_str_mv Alonso-Esteban, José Ignacio
Pinela, José
Pinela, José
https://www.ciencia-id.pt/771C-2B43-B108
771C-2B43-B108
http://orcid.org/0000-0001-7523-1637
0000-0001-7523-1637
Soković, Marina
Barros, Lillian
Barros, Lillian
https://www.ciencia-id.pt/9616-35CB-D001
9616-35CB-D001
http://orcid.org/0000-0002-9050-5189
0000-0002-9050-5189
Sánchez-Mata, María Cortes
Torija-Isasa, Esperanza
Ferreira, Isabel C.F.R.
Ferreira, Isabel C.F.R.
https://www.ciencia-id.pt/9418-CF95-9919
9418-CF95-9919
http://orcid.org/0000-0003-4910-4882
0000-0003-4910-4882
publishDate 2018
reponame_str Biblioteca Digital do IPB
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service_str_mv urn:repositoryAcronym:ipb
spelling porpt_PTThe European hackberry (Celtis australis L.) is a deciduous tree of the Cannabaceae family frequently found in southern Europe and western Asia. Its fruit is an edible, dark-purple berry-like drupe with a large seed that corresponds to about 38% of the fruit dry weight [ l ]. Fruit preparations are used in traditional medicine as a remedy for colic, peptic ulcers, dysentery, diarrhea, amenorrhea, and heavy menstrual bleeding [1-3]. However, as far as we know, the antioxidant and antimicrobial properties of C. australis seeds have never been explored before, being this the purpose ofthis study. The C. australis seed extract was obtained by a solid-liquid extraction of the powdered sample with methanol water (80:20, v/v) [4]. Then, the antioxidant activity was measured in vitro via DPPH' scavenging activity, reducing power, p-carotene bleaching inhibition capacity, and thiobarbituric acid reactive substances (TBARS) formation inhibition capacity in brain cell homogenates [4]. The antimicrobial activity was screened against twelve microorganisms, including Gram-positive and Gram-negative bactéria and fungi, by the microdilution method [5]. The seed extract was effective in inhibiting the formation of TBARS as byproducts Of lipid peroxidation, which was concluded from the low EC^ value. The extract also had an interesting reducing power and capacity to protect p-carotene from the free radicais generated from linoleic acid. Regarding its antimicrobial activity, the best results were against B. cereus, with MIC and MBC lower than ampicillin, A. ochraceus, with MIC and MFC lower than ketoconazole, and P. funiculosum, with MIC lower than ketoconazole and bifonazole and MFC lower than ketoconazole. These results highlighted the potential of C. australis seeds as an interesting fruit byproduct to be used in the development of bio-based antimicrobials.application/pdfpt_PTAntioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seedsAlonso-Esteban, José IgnacioPersonalPinela, JoséDSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4DSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4PinelaJoséCiência IDhttps://www.ciencia-id.pt771C-2B43-B108ORCIDhttp://orcid.org0000-0001-7523-1637Researcher IDhttps://www.researcherid.comB-4466-2014Scopus Author IDhttps://www.scopus.com54392272800Soković, MarinaPersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801Sánchez-Mata, María CortesTorija-Isasa, EsperanzaPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-989-8124-24-12019-01-07T12:13:55Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/18311http://purl.org/coar/access_right/c_abf2open accessEuropean hackberryCeltis australís L.332908 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2018http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/5ba74239-df48-4a1c-9c3f-9c671525e1e2/downloadXXIV Encontro Luso Galego de QuímicaPorto, Portugal
spellingShingle Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
Alonso-Esteban, José Ignacio
European hackberry
Celtis australís L.
status SINGLETON
subject.fl_str_mv European hackberry
Celtis australís L.
title Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
title_full Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
title_fullStr Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
title_full_unstemmed Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
title_short Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
title_sort Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seeds
topic European hackberry
Celtis australís L.
topic_facet European hackberry
Celtis australís L.
url http://hdl.handle.net/10198/18311
visible 1