Publicação
Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements
| Resumo: | Trypanosomatids form a group of high prevalence protozoa that parasitise honey bees, with Lotmaria passim as the predominant species worldwide. However, the knowledge about the ecology of trypanosomatids in isolated areas is limited. The Portuguese archipelagos of Madeira and Azores provide an interesting setting to investigate these parasites because of their geographic isolation, and because they harbour honey bee populations devoid of two major enemies: Varroa destructor and Nosema ceranae. Hence, a total of 661 honey bee colonies from Madeira and the Azores were analysed using different molecular techniques, through which we found a high prevalence of trypanosomatids despite the isolation of these islands. L. passim was the predominant species and, in most colonies, was the only one found, even on islands free of V. destructor and/or N. ceranae with severe restrictions on colony movements to prevent the spread of them. However, islands with V. destructor had a significantly higher prevalence of L. passim and, conversely, islands with N. ceranae had a significantly lower prevalence of the trypanosomatid. Crithidia bombi was detected in Madeira and on three islands of the Azores, almost always coincident with L. passim. By contrast, Crithidia mellificae was not detected in any sample. A High-Throughput Sequencing analysis distinguished two main haplotypes of L. passim, which accounted for 98% of the total sequence reads. This work suggests that L. passim and C. bombi are parasites that have been associated with honey bees predating the spread of V. destructor and N. ceranae. |
|---|---|
| Autores principais: | Martin Hernández, Raquel |
| Outros Autores: | Aguado-López, Daniel; Bartolomé, Carolina; Lopes, Ana; Henriques, Dora; Maside, Xulio; Pinto, M. Alice; Higes, Mariano |
| Assunto: | Trypanosomatids Apis mellifera |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173344221069312 |
|---|---|
| author | Martin Hernández, Raquel |
| author2 | Aguado-López, Daniel Bartolomé, Carolina Lopes, Ana Henriques, Dora Maside, Xulio Pinto, M. Alice Higes, Mariano |
| author2_role | author author author author author author author |
| author_facet | Martin Hernández, Raquel Aguado-López, Daniel Bartolomé, Carolina Lopes, Ana Henriques, Dora Maside, Xulio Pinto, M. Alice Higes, Mariano |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Martin Hernández, Raquel\"},{\"Person.name\":\"Aguado-López, Daniel\"},{\"Person.name\":\"Bartolomé, Carolina\"},{\"Person.name\":\"Lopes, Ana\",\"Person.identifier.orcid\":\"0000-0002-1888-2262\"},{\"Person.name\":\"Henriques, Dora\",\"Person.identifier.orcid\":\"0000-0001-7530-682X\"},{\"Person.name\":\"Maside, Xulio\"},{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"},{\"Person.name\":\"Higes, Mariano\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Martin Hernández, Raquel Aguado-López, Daniel Bartolomé, Carolina Lopes, Ana Henriques, Dora Maside, Xulio Pinto, M. Alice Higes, Mariano |
| datacite.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-10-20T15:23:45Z |
| datacite.date.embargoed.fl_str_mv | 2023-10-20T15:23:45Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Trypanosomatids Apis mellifera |
| datacite.titles.title.fl_str_mv | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Martin Hernández, Raquel Aguado-López, Daniel Bartolomé, Carolina Lopes, Ana Henriques, Dora Maside, Xulio Pinto, M. Alice Higes, Mariano |
| dc.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-10-20T15:23:45Z |
| dc.date.embargoed.fl_str_mv | 2023-10-20T15:23:45Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/28812 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Apimondia |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Trypanosomatids Apis mellifera |
| dc.title.fl_str_mv | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Trypanosomatids form a group of high prevalence protozoa that parasitise honey bees, with Lotmaria passim as the predominant species worldwide. However, the knowledge about the ecology of trypanosomatids in isolated areas is limited. The Portuguese archipelagos of Madeira and Azores provide an interesting setting to investigate these parasites because of their geographic isolation, and because they harbour honey bee populations devoid of two major enemies: Varroa destructor and Nosema ceranae. Hence, a total of 661 honey bee colonies from Madeira and the Azores were analysed using different molecular techniques, through which we found a high prevalence of trypanosomatids despite the isolation of these islands. L. passim was the predominant species and, in most colonies, was the only one found, even on islands free of V. destructor and/or N. ceranae with severe restrictions on colony movements to prevent the spread of them. However, islands with V. destructor had a significantly higher prevalence of L. passim and, conversely, islands with N. ceranae had a significantly lower prevalence of the trypanosomatid. Crithidia bombi was detected in Madeira and on three islands of the Azores, almost always coincident with L. passim. By contrast, Crithidia mellificae was not detected in any sample. A High-Throughput Sequencing analysis distinguished two main haplotypes of L. passim, which accounted for 98% of the total sequence reads. This work suggests that L. passim and C. bombi are parasites that have been associated with honey bees predating the spread of V. destructor and N. ceranae. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
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| language | eng |
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| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Martin Hernández, Raquel Aguado-López, Daniel Bartolomé, Carolina Lopes, Ana Lopes, Ana https://www.ciencia-id.pt/F014-6835-054D F014-6835-054D http://orcid.org/0000-0002-1888-2262 0000-0002-1888-2262 Henriques, Dora Henriques, Dora https://www.ciencia-id.pt/291F-986F-07DA 291F-986F-07DA http://orcid.org/0000-0001-7530-682X 0000-0001-7530-682X Maside, Xulio Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 Higes, Mariano |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Apimondia |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engApimondiaenTrypanosomatids form a group of high prevalence protozoa that parasitise honey bees, with Lotmaria passim as the predominant species worldwide. However, the knowledge about the ecology of trypanosomatids in isolated areas is limited. The Portuguese archipelagos of Madeira and Azores provide an interesting setting to investigate these parasites because of their geographic isolation, and because they harbour honey bee populations devoid of two major enemies: Varroa destructor and Nosema ceranae. Hence, a total of 661 honey bee colonies from Madeira and the Azores were analysed using different molecular techniques, through which we found a high prevalence of trypanosomatids despite the isolation of these islands. L. passim was the predominant species and, in most colonies, was the only one found, even on islands free of V. destructor and/or N. ceranae with severe restrictions on colony movements to prevent the spread of them. However, islands with V. destructor had a significantly higher prevalence of L. passim and, conversely, islands with N. ceranae had a significantly lower prevalence of the trypanosomatid. Crithidia bombi was detected in Madeira and on three islands of the Azores, almost always coincident with L. passim. By contrast, Crithidia mellificae was not detected in any sample. A High-Throughput Sequencing analysis distinguished two main haplotypes of L. passim, which accounted for 98% of the total sequence reads. This work suggests that L. passim and C. bombi are parasites that have been associated with honey bees predating the spread of V. destructor and N. ceranae.application/pdfpt_PTFrequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movementsMartin Hernández, RaquelAguado-López, DanielBartolomé, CarolinaPersonalLopes, AnaDSpacehttp://dspace.org/items/5c8dc714-e63c-4ac6-882e-92deafa4bbe8DSpacehttp://dspace.org/items/5c8dc714-e63c-4ac6-882e-92deafa4bbe8LopesAna RitaCiência IDhttps://www.ciencia-id.ptF014-6835-054DORCIDhttp://orcid.org0000-0002-1888-2262PersonalHenriques, DoraDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dHenriquesDoraCiência IDhttps://www.ciencia-id.pt291F-986F-07DAORCIDhttp://orcid.org0000-0001-7530-682XScopus Author IDhttps://www.scopus.com55761737300Maside, XulioPersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800Higes, MarianoHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2023-10-20T15:23:45Z20232023-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/28812http://purl.org/coar/access_right/c_abf2open accessTrypanosomatidsApis mellifera231802 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2023http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/3a8e137e-6014-4b38-846c-aee7603af335/downloadApimondia 48th International Apicultural Congress6969Chile |
| spellingShingle | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements Martin Hernández, Raquel Trypanosomatids Apis mellifera |
| status | SINGLETON |
| subject.fl_str_mv | Trypanosomatids Apis mellifera |
| title | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| title_full | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| title_fullStr | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| title_full_unstemmed | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| title_short | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| title_sort | Frequent parasitism of Apis mellifera by trypanosomatids in geographically isolated areas with restricted beekeeping movements |
| topic | Trypanosomatids Apis mellifera |
| topic_facet | Trypanosomatids Apis mellifera |
| url | http://hdl.handle.net/10198/28812 |
| visible | 1 |