Publicação
Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts
| Resumo: | Pterospartum tridentatum Willk. (prickled broom) is a common autochthonous plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was decreased, indicating a dysfunction in respiratory activity induced by P. tridentatum leaves extract and, in good agreement with the previous results cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract.10-6 cells) showed a decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves in high amounts or continuously should be regarded as potencially noxious. |
|---|---|
| Autores principais: | Ferreira, Fernanda M. |
| Outros Autores: | Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Palmeira, Carlos M.; Cardoso, Susana M. |
| Assunto: | Pterospartum tridentatum Cytotoxicity Mitochondria effects |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172854327410688 |
|---|---|
| author | Ferreira, Fernanda M. |
| author2 | Peixoto, Francisco P. Pereira, Olívia R. Dinis, Lia Tânia Domingues, Maria R.M. Palmeira, Carlos M. Cardoso, Susana M. |
| author2_role | author author author author author author |
| author_facet | Ferreira, Fernanda M. Peixoto, Francisco P. Pereira, Olívia R. Dinis, Lia Tânia Domingues, Maria R.M. Palmeira, Carlos M. Cardoso, Susana M. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ferreira, Fernanda M.\"},{\"Person.name\":\"Peixoto, Francisco P.\"},{\"Person.name\":\"Pereira, Olívia R.\",\"Person.identifier.orcid\":\"0000-0002-6275-3134\"},{\"Person.name\":\"Dinis, Lia Tânia\"},{\"Person.name\":\"Domingues, Maria R.M.\"},{\"Person.name\":\"Palmeira, Carlos M.\"},{\"Person.name\":\"Cardoso, Susana M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Ferreira, Fernanda M. Peixoto, Francisco P. Pereira, Olívia R. Dinis, Lia Tânia Domingues, Maria R.M. Palmeira, Carlos M. Cardoso, Susana M. |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-07-03T14:59:47Z |
| datacite.date.embargoed.fl_str_mv | 2015-07-03T14:59:47Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Pterospartum tridentatum Cytotoxicity Mitochondria effects |
| datacite.titles.title.fl_str_mv | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Ferreira, Fernanda M. Peixoto, Francisco P. Pereira, Olívia R. Dinis, Lia Tânia Domingues, Maria R.M. Palmeira, Carlos M. Cardoso, Susana M. |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-07-03T14:59:47Z |
| dc.date.embargoed.fl_str_mv | 2015-07-03T14:59:47Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/11926 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Instituto Nacional Doutor Ricardo Jorge |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Pterospartum tridentatum Cytotoxicity Mitochondria effects |
| dc.title.fl_str_mv | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Pterospartum tridentatum Willk. (prickled broom) is a common autochthonous plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was decreased, indicating a dysfunction in respiratory activity induced by P. tridentatum leaves extract and, in good agreement with the previous results cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract.10-6 cells) showed a decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves in high amounts or continuously should be regarded as potencially noxious. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/9ecff2d7-5295-42ab-b702-d4f3a4c6a18d/download |
| id | ipb_02ae32b3cca68ea4821178ef3cd967aa |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/11926 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/11926 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Ferreira, Fernanda M. Peixoto, Francisco P. Pereira, Olívia R. Pereira, Olívia R. https://www.ciencia-id.pt/B312-AF21-D2FA B312-AF21-D2FA http://orcid.org/0000-0002-6275-3134 0000-0002-6275-3134 Dinis, Lia Tânia Domingues, Maria R.M. Palmeira, Carlos M. Cardoso, Susana M. |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Instituto Nacional Doutor Ricardo Jorge |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engInstituto Nacional Doutor Ricardo JorgeporPterospartum tridentatum Willk. (prickled broom) is a common autochthonous plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was decreased, indicating a dysfunction in respiratory activity induced by P. tridentatum leaves extract and, in good agreement with the previous results cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract.10-6 cells) showed a decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves in high amounts or continuously should be regarded as potencially noxious.application/pdfporToxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extractsFerreira, Fernanda M.Peixoto, Francisco P.PersonalPereira, Olívia R.DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453PereiraOlívia R.Ciência IDhttps://www.ciencia-id.ptB312-AF21-D2FAORCIDhttp://orcid.org0000-0002-6275-3134Researcher IDhttps://www.researcherid.comH-1049-2014Scopus Author IDhttps://www.scopus.com52264387000Dinis, Lia TâniaDomingues, Maria R.M.Palmeira, Carlos M.Cardoso, Susana M.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2015-07-03T14:59:47Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/11926http://purl.org/coar/access_right/c_abf2open accessPterospartum tridentatumCytotoxicityMitochondria effects1368075 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/9ecff2d7-5295-42ab-b702-d4f3a4c6a18d/downloadInternational Conference on Food Contaminants: challenges in chemical mixtures173174Lisboa, Portugal |
| spellingShingle | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts Ferreira, Fernanda M. Pterospartum tridentatum Cytotoxicity Mitochondria effects |
| status | SINGLETON |
| subject.fl_str_mv | Pterospartum tridentatum Cytotoxicity Mitochondria effects |
| title | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| title_full | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| title_fullStr | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| title_full_unstemmed | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| title_short | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| title_sort | Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extracts |
| topic | Pterospartum tridentatum Cytotoxicity Mitochondria effects |
| topic_facet | Pterospartum tridentatum Cytotoxicity Mitochondria effects |
| url | http://hdl.handle.net/10198/11926 |
| visible | 1 |