Publicação
In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds
| Resumo: | In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH•. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits. |
|---|---|
| Autores principais: | Taveira, Marcos |
| Outros Autores: | Pereira, David M.; Sousa, Carla; Ferreres, Frederico; Andrade, Paula B.; Martins, Anabela; Pereira, J.A.; Valentão, Patrícia |
| Assunto: | Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851192821481472 |
|---|---|
| author | Taveira, Marcos |
| author2 | Pereira, David M. Sousa, Carla Ferreres, Frederico Andrade, Paula B. Martins, Anabela Pereira, J.A. Valentão, Patrícia |
| author2_role | author author author author author author author |
| author_facet | Taveira, Marcos Pereira, David M. Sousa, Carla Ferreres, Frederico Andrade, Paula B. Martins, Anabela Pereira, J.A. Valentão, Patrícia |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Taveira, Marcos\"},{\"Person.name\":\"Pereira, David M.\"},{\"Person.name\":\"Sousa, Carla\"},{\"Person.name\":\"Ferreres, Frederico\"},{\"Person.name\":\"Andrade, Paula B.\"},{\"Person.name\":\"Martins, Anabela\",\"Person.identifier.orcid\":\"0000-0001-6218-4413\"},{\"Person.name\":\"Pereira, J.A.\",\"Person.identifier.orcid\":\"0000-0002-2260-0600\"},{\"Person.name\":\"Valentão, Patrícia\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Taveira, Marcos Pereira, David M. Sousa, Carla Ferreres, Frederico Andrade, Paula B. Martins, Anabela Pereira, J.A. Valentão, Patrícia |
| datacite.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-01-10T16:17:54Z |
| datacite.date.embargoed.fl_str_mv | 2011-01-10T16:17:54Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity |
| datacite.titles.title.fl_str_mv | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Taveira, Marcos Pereira, David M. Sousa, Carla Ferreres, Frederico Andrade, Paula B. Martins, Anabela Pereira, J.A. Valentão, Patrícia |
| dc.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-01-10T16:17:54Z |
| dc.date.embargoed.fl_str_mv | 2011-01-10T16:17:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/3043 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | ACS |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity |
| dc.title.fl_str_mv | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH•. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/dd52ac76-adc8-4563-b074-d73902433702/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 5876-PPCDTI |
| id | ipb_03a2ccf7bfaa5faeb2c8459d700fccc3 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/3043 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/3043 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Taveira, Marcos Pereira, David M. Sousa, Carla Ferreres, Frederico Andrade, Paula B. Martins, Anabela Martins, Anabela https://www.ciencia-id.pt/7B18-A810-6B93 7B18-A810-6B93 http://orcid.org/0000-0001-6218-4413 0000-0001-6218-4413 Pereira, J.A. Pereira, J.A. https://www.ciencia-id.pt/611F-80B2-A7C1 611F-80B2-A7C1 http://orcid.org/0000-0002-2260-0600 0000-0002-2260-0600 Valentão, Patrícia |
| publishDate | 2009 |
| publisher.none.fl_str_mv | ACS |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engACSporIn this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH•. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits.application/pdfporIn vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compoundsTaveira, MarcosPereira, David M.Sousa, CarlaFerreres, FredericoAndrade, Paula B.PersonalMartins, AnabelaDSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581DSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581MartinsAnabelaCiência IDhttps://www.ciencia-id.pt7B18-A810-6B93ORCIDhttp://orcid.org0000-0001-6218-4413Researcher IDhttps://www.researcherid.comG-5488-2013Scopus Author IDhttps://www.scopus.com7203013518PersonalPereira, J.A.DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857PereiraJosé AlbertoCiência IDhttps://www.ciencia-id.pt611F-80B2-A7C1ORCIDhttp://orcid.org0000-0002-2260-0600Researcher IDhttps://www.researcherid.comL-6798-2014Scopus Author IDhttps://www.scopus.com57204366348Valentão, PatríciaHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2011-01-10T16:17:54Z20092009-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/3043http://purl.org/coar/access_right/c_16ecrestricted accessBrassica oleracea L. var. costata DCShootsCalliRootsPhenolic compoundsAntioxidant activity232678 bytesFundação para a Ciência e a TecnologiaPieris brassicae as laboratory of synthesis of new compounds with biological potential, starting from Brassica oleracea var. costata, Brassica oleracea var. acephala and Brassica rapa var. rapa.5876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/dd52ac76-adc8-4563-b074-d73902433702/downloadJournal of Agricultural and Food Chemistry5712471252 |
| spellingShingle | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds Taveira, Marcos Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity |
| subject.fl_str_mv | Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity |
| title | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| title_full | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| title_fullStr | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| title_full_unstemmed | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| title_short | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| title_sort | In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds |
| topic | Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity |
| topic_facet | Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity |
| url | http://hdl.handle.net/10198/3043 |
| visible | 1 |