Publicação
Spatio-temporal patterns of Nosema cerenae in the Azores archipelago
| Resumo: | Nosema ceranae is a highly prevalent pathogen of Apis mellifera, which is distributed worldwide. However, there may still exist geographically isolated areas that remain free of this pathogen. While Nosema spp. spores have been identified in samples from the Azores archipelago since early 2000’s, whether those spores matched N. apis or N. ceranae was unknown until this study. To address this question, we used molecular tools (multiplex PCR and real-time qPCR) to scrutinize 474 colonies sampled from eight islands in 2014/2015 and 91 from four islands in 2020. The findings revealed that N. ceranae was not only present but also the dominant species in the Azores. In 2014/2015, N. apis was rare and N. ceranae prevalence varied between 2.7% in São Jorge and 50.7% in Pico. In 2020, N. ceranae prevalence increased significantly (p < 0.001) in Terceira and São Jorge, which also showed higher infection levels. The spatio-temporal patterns suggest that N. ceranae colonised the archipelago recently, and it rapidly spread across other islands, where at least two independent introductions might have occurred. Flores and Santa Maria have escaped the N. ceranae invasion, and it is remarkable that Santa Maria is also free of Varroa destructor, which makes it one of the last places in Europe where the honey bee remains naive to these two major biotic stressors. |
|---|---|
| Autores principais: | Lopes, Ana |
| Outros Autores: | Martín-Hernández, Raquel; Higes, Mariano; Henriques, Dora; Pinto, M. Alice |
| Assunto: | Nosema ceranae Prevalence Azorean honey bees |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172878798028800 |
|---|---|
| author | Lopes, Ana |
| author2 | Martín-Hernández, Raquel Higes, Mariano Henriques, Dora Pinto, M. Alice |
| author2_role | author author author author |
| author_facet | Lopes, Ana Martín-Hernández, Raquel Higes, Mariano Henriques, Dora Pinto, M. Alice |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Lopes, Ana\",\"Person.identifier.orcid\":\"0000-0002-1888-2262\"},{\"Person.name\":\"Martín-Hernández, Raquel\"},{\"Person.name\":\"Higes, Mariano\"},{\"Person.name\":\"Henriques, Dora\",\"Person.identifier.orcid\":\"0000-0001-7530-682X\"},{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Lopes, Ana Martín-Hernández, Raquel Higes, Mariano Henriques, Dora Pinto, M. Alice |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-10-17T13:41:27Z |
| datacite.date.embargoed.fl_str_mv | 2022-10-17T13:41:27Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Nosema ceranae Prevalence Azorean honey bees |
| datacite.titles.title.fl_str_mv | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Lopes, Ana Martín-Hernández, Raquel Higes, Mariano Henriques, Dora Pinto, M. Alice |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-10-17T13:41:27Z |
| dc.date.embargoed.fl_str_mv | 2022-10-17T13:41:27Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/26019 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Estonian University of Life Science |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Nosema ceranae Prevalence Azorean honey bees |
| dc.title.fl_str_mv | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Nosema ceranae is a highly prevalent pathogen of Apis mellifera, which is distributed worldwide. However, there may still exist geographically isolated areas that remain free of this pathogen. While Nosema spp. spores have been identified in samples from the Azores archipelago since early 2000’s, whether those spores matched N. apis or N. ceranae was unknown until this study. To address this question, we used molecular tools (multiplex PCR and real-time qPCR) to scrutinize 474 colonies sampled from eight islands in 2014/2015 and 91 from four islands in 2020. The findings revealed that N. ceranae was not only present but also the dominant species in the Azores. In 2014/2015, N. apis was rare and N. ceranae prevalence varied between 2.7% in São Jorge and 50.7% in Pico. In 2020, N. ceranae prevalence increased significantly (p < 0.001) in Terceira and São Jorge, which also showed higher infection levels. The spatio-temporal patterns suggest that N. ceranae colonised the archipelago recently, and it rapidly spread across other islands, where at least two independent introductions might have occurred. Flores and Santa Maria have escaped the N. ceranae invasion, and it is remarkable that Santa Maria is also free of Varroa destructor, which makes it one of the last places in Europe where the honey bee remains naive to these two major biotic stressors. |
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| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/6ff8b26c-ba8d-4c29-ba95-29c0d3ce3743/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/26019 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| person_str_mv | Lopes, Ana Lopes, Ana https://www.ciencia-id.pt/F014-6835-054D F014-6835-054D http://orcid.org/0000-0002-1888-2262 0000-0002-1888-2262 Martín-Hernández, Raquel Higes, Mariano Henriques, Dora Henriques, Dora https://www.ciencia-id.pt/291F-986F-07DA 291F-986F-07DA http://orcid.org/0000-0001-7530-682X 0000-0001-7530-682X Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Estonian University of Life Science |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
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| spelling | engEstonian University of Life Sciencept_PTNosema ceranae is a highly prevalent pathogen of Apis mellifera, which is distributed worldwide. However, there may still exist geographically isolated areas that remain free of this pathogen. While Nosema spp. spores have been identified in samples from the Azores archipelago since early 2000’s, whether those spores matched N. apis or N. ceranae was unknown until this study. To address this question, we used molecular tools (multiplex PCR and real-time qPCR) to scrutinize 474 colonies sampled from eight islands in 2014/2015 and 91 from four islands in 2020. The findings revealed that N. ceranae was not only present but also the dominant species in the Azores. In 2014/2015, N. apis was rare and N. ceranae prevalence varied between 2.7% in São Jorge and 50.7% in Pico. In 2020, N. ceranae prevalence increased significantly (p < 0.001) in Terceira and São Jorge, which also showed higher infection levels. The spatio-temporal patterns suggest that N. ceranae colonised the archipelago recently, and it rapidly spread across other islands, where at least two independent introductions might have occurred. Flores and Santa Maria have escaped the N. ceranae invasion, and it is remarkable that Santa Maria is also free of Varroa destructor, which makes it one of the last places in Europe where the honey bee remains naive to these two major biotic stressors.application/pdfpt_PTSpatio-temporal patterns of Nosema cerenae in the Azores archipelagoPersonalLopes, AnaDSpacehttp://dspace.org/items/5c8dc714-e63c-4ac6-882e-92deafa4bbe8DSpacehttp://dspace.org/items/5c8dc714-e63c-4ac6-882e-92deafa4bbe8LopesAnaCiência IDhttps://www.ciencia-id.ptF014-6835-054DORCIDhttp://orcid.org0000-0002-1888-2262Martín-Hernández, RaquelHiges, MarianoPersonalHenriques, DoraDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dHenriquesDoraCiência IDhttps://www.ciencia-id.pt291F-986F-07DAORCIDhttp://orcid.org0000-0001-7530-682XScopus Author IDhttps://www.scopus.com55761737300PersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2022-10-17T13:41:27Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/26019http://purl.org/coar/access_right/c_abf2open accessNosema ceranaePrevalenceAzorean honey bees115453 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/6ff8b26c-ba8d-4c29-ba95-29c0d3ce3743/downloadEurbee 9: 9th European Conference of Apidology141141Belgrade |
| spellingShingle | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago Lopes, Ana Nosema ceranae Prevalence Azorean honey bees |
| status | SINGLETON |
| subject.fl_str_mv | Nosema ceranae Prevalence Azorean honey bees |
| title | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| title_full | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| title_fullStr | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| title_full_unstemmed | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| title_short | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| title_sort | Spatio-temporal patterns of Nosema cerenae in the Azores archipelago |
| topic | Nosema ceranae Prevalence Azorean honey bees |
| topic_facet | Nosema ceranae Prevalence Azorean honey bees |
| url | http://hdl.handle.net/10198/26019 |
| visible | 1 |