Publicação
Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR
| Resumo: | The need for reliable and sensitive methods for meat species identification in ground and comminuted meat products encompasses many issues including the fraudulent substitution of higher value species by cheaper meats species, the presence of undeclared species, the use of lower amounts of meat than the quantities declared on the product’s label and the substitution of meat by lower price vegetable proteins. This can represent an important economic fraud, but can also bring ethical, religious and even health repercussions. Several techniques are currently used for meat species identification in complex mixtures, including different protein-based methods such as HPLC, ELISA and electrophoretic techniques. Nevertheless, in processed meat products, these methods can be significantly less sensitive for the detection of specific species due to heat denaturation of proteins during industrial thermal processing. |
|---|---|
| Autores principais: | Mafra, Isabel |
| Outros Autores: | Amaral, Joana S.; Soares, Sónia; Oliveira, Beatriz |
| Assunto: | Species identification Meat products Real-time PCR |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850797838630912 |
|---|---|
| author | Mafra, Isabel |
| author2 | Amaral, Joana S. Soares, Sónia Oliveira, Beatriz |
| author2_role | author author author |
| author_facet | Mafra, Isabel Amaral, Joana S. Soares, Sónia Oliveira, Beatriz |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Mafra, Isabel\"},{\"Person.name\":\"Amaral, Joana S.\",\"Person.identifier.orcid\":\"0000-0002-3648-7303\"},{\"Person.name\":\"Soares, Sónia\"},{\"Person.name\":\"Oliveira, Beatriz\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Mafra, Isabel Amaral, Joana S. Soares, Sónia Oliveira, Beatriz |
| datacite.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-06-03T09:06:26Z |
| datacite.date.embargoed.fl_str_mv | 2011-06-03T09:06:26Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Species identification Meat products Real-time PCR |
| datacite.titles.title.fl_str_mv | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Mafra, Isabel Amaral, Joana S. Soares, Sónia Oliveira, Beatriz |
| dc.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-06-03T09:06:26Z |
| dc.date.embargoed.fl_str_mv | 2011-06-03T09:06:26Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/4951 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Species identification Meat products Real-time PCR |
| dc.title.fl_str_mv | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | The need for reliable and sensitive methods for meat species identification in ground and comminuted meat products encompasses many issues including the fraudulent substitution of higher value species by cheaper meats species, the presence of undeclared species, the use of lower amounts of meat than the quantities declared on the product’s label and the substitution of meat by lower price vegetable proteins. This can represent an important economic fraud, but can also bring ethical, religious and even health repercussions. Several techniques are currently used for meat species identification in complex mixtures, including different protein-based methods such as HPLC, ELISA and electrophoretic techniques. Nevertheless, in processed meat products, these methods can be significantly less sensitive for the detection of specific species due to heat denaturation of proteins during industrial thermal processing. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/9b9cf9ac-0a91-429c-a71a-6cd74475851f/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/4951 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/4951 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Mafra, Isabel Amaral, Joana S. Amaral, Joana S. https://www.ciencia-id.pt/5319-7DE8-BEDA 5319-7DE8-BEDA http://orcid.org/0000-0002-3648-7303 0000-0002-3648-7303 Soares, Sónia Oliveira, Beatriz |
| publishDate | 2009 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engporThe need for reliable and sensitive methods for meat species identification in ground and comminuted meat products encompasses many issues including the fraudulent substitution of higher value species by cheaper meats species, the presence of undeclared species, the use of lower amounts of meat than the quantities declared on the product’s label and the substitution of meat by lower price vegetable proteins. This can represent an important economic fraud, but can also bring ethical, religious and even health repercussions. Several techniques are currently used for meat species identification in complex mixtures, including different protein-based methods such as HPLC, ELISA and electrophoretic techniques. Nevertheless, in processed meat products, these methods can be significantly less sensitive for the detection of specific species due to heat denaturation of proteins during industrial thermal processing.application/pdfporQuantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCRMafra, IsabelPersonalAmaral, Joana S.DSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdDSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdAmaralJoana S.Ciência IDhttps://www.ciencia-id.pt5319-7DE8-BEDAORCIDhttp://orcid.org0000-0002-3648-7303Soares, SóniaOliveira, BeatrizHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2011-06-03T09:06:26Z20092009-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/4951http://purl.org/coar/access_right/c_abf2open accessSpecies identificationMeat productsReal-time PCR144874 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/9b9cf9ac-0a91-429c-a71a-6cd74475851f/downloadFinal TRACE Conference: “How to trace the origin of food?”Bruxelas |
| spellingShingle | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR Mafra, Isabel Species identification Meat products Real-time PCR |
| subject.fl_str_mv | Species identification Meat products Real-time PCR |
| title | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| title_full | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| title_fullStr | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| title_full_unstemmed | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| title_short | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| title_sort | Quantitative detection of pork’s meat adulteration in processed poultry’s meat products by real time PCR |
| topic | Species identification Meat products Real-time PCR |
| topic_facet | Species identification Meat products Real-time PCR |
| url | http://hdl.handle.net/10198/4951 |
| visible | 1 |