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Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems

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Resumo:Three different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils. To support the validity of the method, the parameters evaluated were linearity, detection limits, repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six identified compounds: R-T, -T, γ-T, δ-T, R-T3, and γ-T3. R-Tocopherol (R-T) was the main vitamin E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol composition of the olive oils under evaluation.
Autores principais:Cunha, Sara C.
Outros Autores:Amaral, Joana S.; Fernandes, J.O.; Oliveira, Beatriz
Assunto:Tocopherols Tocotrienols Olive oil NP-HPLC
Ano:2006
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB

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