Publicação
New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants
| Resumo: | Artichoke (A), borututu (B) and milk thistle (M) are included in several supplements to provide beneficial effects. Different formulations (infusions, pills and syrups), as also different proportions of A, B and M (1:1:1, 2:1:1, 1:2:1, 1:1:2) within each formulation were assayed to optimize the desired benefits. The antioxidant activity, anti-hepatocellular carcinoma activity, hepatotoxicity and bioactive compounds contents were evaluated. Syrups tended to be the formulation with highest antioxidant activity and total phenolics and flavonoids content; otherwise, pills were the worst formulation. In what concerns A:B:M ratios, the results did not reveal so pronounced differences. None of the assayed mixtures resulted to be toxic (up to the maximum assayed dose) for liver primary cells (PLP2), but some samples, especially infusions, showed toxicity for the hepatocellular carcinoma cell line (HepG2). With no exception, the mixtures for all formulations gave synergistic effects in antioxidant activity, when compared to the activity of single plants. |
|---|---|
| Autores principais: | Pereira, Carla |
| Outros Autores: | Barreira, João C.M.; Calhelha, Ricardo C.; Queiroz, Maria João R.P.; Barros, Lillian; Ferreira, Isabel C.F.R. |
| Assunto: | Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173366718267392 |
|---|---|
| author | Pereira, Carla |
| author2 | Barreira, João C.M. Calhelha, Ricardo C. Queiroz, Maria João R.P. Barros, Lillian Ferreira, Isabel C.F.R. |
| author2_role | author author author author author |
| author_facet | Pereira, Carla Barreira, João C.M. Calhelha, Ricardo C. Queiroz, Maria João R.P. Barros, Lillian Ferreira, Isabel C.F.R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pereira, Carla\",\"Person.identifier.orcid\":\"0000-0003-0093-771X\"},{\"Person.name\":\"Barreira, João C.M.\",\"Person.identifier.orcid\":\"0000-0003-1233-0990\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Queiroz, Maria João R.P.\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pereira, Carla Barreira, João C.M. Calhelha, Ricardo C. Queiroz, Maria João R.P. Barros, Lillian Ferreira, Isabel C.F.R. |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-08-04T09:39:56Z |
| datacite.date.embargoed.fl_str_mv | 2015-08-04T09:39:56Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism |
| datacite.titles.title.fl_str_mv | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pereira, Carla Barreira, João C.M. Calhelha, Ricardo C. Queiroz, Maria João R.P. Barros, Lillian Ferreira, Isabel C.F.R. |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-08-04T09:39:56Z |
| dc.date.embargoed.fl_str_mv | 2015-08-04T09:39:56Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/12032 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Royal Society of Chemistry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism |
| dc.title.fl_str_mv | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Artichoke (A), borututu (B) and milk thistle (M) are included in several supplements to provide beneficial effects. Different formulations (infusions, pills and syrups), as also different proportions of A, B and M (1:1:1, 2:1:1, 1:2:1, 1:1:2) within each formulation were assayed to optimize the desired benefits. The antioxidant activity, anti-hepatocellular carcinoma activity, hepatotoxicity and bioactive compounds contents were evaluated. Syrups tended to be the formulation with highest antioxidant activity and total phenolics and flavonoids content; otherwise, pills were the worst formulation. In what concerns A:B:M ratios, the results did not reveal so pronounced differences. None of the assayed mixtures resulted to be toxic (up to the maximum assayed dose) for liver primary cells (PLP2), but some samples, especially infusions, showed toxicity for the hepatocellular carcinoma cell line (HepG2). With no exception, the mixtures for all formulations gave synergistic effects in antioxidant activity, when compared to the activity of single plants. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/b720768f-3fcb-411b-ad22-49713aad6cf9/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ipb_1f1ee2b4e8c697d7e382f45c642ced2b |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/12032 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/12032 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pereira, Carla Pereira, Carla https://www.ciencia-id.pt/EF10-2739-2B70 EF10-2739-2B70 http://orcid.org/0000-0003-0093-771X 0000-0003-0093-771X Barreira, João C.M. Barreira, João C.M. http://orcid.org/0000-0003-1233-0990 0000-0003-1233-0990 Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Queiroz, Maria João R.P. Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 |
| publishDate | 2014 |
| publisher.none.fl_str_mv | Royal Society of Chemistry |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engRoyal Society of ChemistryporArtichoke (A), borututu (B) and milk thistle (M) are included in several supplements to provide beneficial effects. Different formulations (infusions, pills and syrups), as also different proportions of A, B and M (1:1:1, 2:1:1, 1:2:1, 1:1:2) within each formulation were assayed to optimize the desired benefits. The antioxidant activity, anti-hepatocellular carcinoma activity, hepatotoxicity and bioactive compounds contents were evaluated. Syrups tended to be the formulation with highest antioxidant activity and total phenolics and flavonoids content; otherwise, pills were the worst formulation. In what concerns A:B:M ratios, the results did not reveal so pronounced differences. None of the assayed mixtures resulted to be toxic (up to the maximum assayed dose) for liver primary cells (PLP2), but some samples, especially infusions, showed toxicity for the hepatocellular carcinoma cell line (HepG2). With no exception, the mixtures for all formulations gave synergistic effects in antioxidant activity, when compared to the activity of single plants.application/pdfapplication/pdfporNew insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plantsPersonalPereira, CarlaDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bPereiraCarlaCiência IDhttps://www.ciencia-id.ptEF10-2739-2B70ORCIDhttp://orcid.org0000-0003-0093-771XResearcher IDhttps://www.researcherid.comK-1629-2016Scopus Author IDhttps://www.scopus.com55627876424PersonalBarreira, João C.M.DSpacehttp://dspace.org/items/4629b12c-39b0-4da8-8b8d-6efba5cf2d81DSpacehttp://dspace.org/items/4629b12c-39b0-4da8-8b8d-6efba5cf2d81BarreiraJoão C.M.ORCIDhttp://orcid.org0000-0003-1233-0990Researcher IDhttps://www.researcherid.comD-8269-2013Scopus Author IDhttps://www.scopus.com54895546900PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333Queiroz, Maria João R.P.PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2042-6496DOIIsPartOf10.1039/c4fo00387j2015-08-04T09:39:56Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/12032http://purl.org/coar/access_right/c_abf2open accessDietary supplementsFormulationCompositional mixtureBioactivityCitotoxicitySynergism622061 bytes560095 bytesFundação para a Ciência e a TecnologiaStrategic Project - UI 690 - 2011-20126817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYSCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/b720768f-3fcb-411b-ad22-49713aad6cf9/downloadhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/9b1b536a-0c49-4d77-b585-16d49df6da87/downloadFood & Function520522060 |
| spellingShingle | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants Pereira, Carla Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism |
| status | SINGLETON |
| subject.fl_str_mv | Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism |
| title | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| title_full | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| title_fullStr | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| title_full_unstemmed | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| title_short | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| title_sort | New insights on the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants |
| topic | Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism |
| topic_facet | Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism |
| url | http://hdl.handle.net/10198/12032 |
| visible | 1 |