Publicação
Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator
| Resumo: | Coastal environments have been recognized as key reservoirs for antibiotic-resistant bacteria. The present study evaluated marine gastropods, Phorcus lineatus, as potential bioindicators to assess the spread of antibiotic-resistant bacteria. P. lineatus was sampled in four sites, with different anthropogenic pressures, along the northwest Portuguese coastal area. From these specimens, bacteria were isolated and tested for their antimicrobial susceptibility, followed by their phylogenetic and pathotypic determination. All the Escherichia coli isolates showed resistance to at least one antimicrobial agent. The highest levels of multidrug resistance (25%) were observed in E. coli isolates obtained from SITE 2, which is impacted by the city of Porto and industrial settlements, while nearly 17% of these isolates showed a multiple antibiotic resistance (MAR) index higher than 0.2. Among the isolates, phylogroups A and B2 were the most prevalent, followed by phylogroup B1. The isolates of phylogroup A showed a higher prevalence of antimicrobial resistance. This study offers valuable insights into the antibiotic resistance risks posed to marine ecosystems and underscores the need for microbiological monitoring and the development of effective management strategies. The findings suggest P. lineatus as a potential bioindicator of antibiotic-resistant bacteria in marine environments. |
|---|---|
| Autores principais: | Santos, Dércia |
| Outros Autores: | Pinto, Ana Rita; Barata, Rita; Fernandes, Conceição; Guedes, Hugo; Almeida, Gonçalo; Cabecinha, Edna; Monteiro, Sandra M.; Varandas, Simone; Saavedra, Maria José |
| Assunto: | Marine gastropods Coastal areas Antibiotics susceptibility Pathotypes Escherichia coli |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173344800931840 |
|---|---|
| author | Santos, Dércia |
| author2 | Pinto, Ana Rita Barata, Rita Fernandes, Conceição Guedes, Hugo Almeida, Gonçalo Cabecinha, Edna Monteiro, Sandra M. Varandas, Simone Saavedra, Maria José |
| author2_role | author author author author author author author author author |
| author_facet | Santos, Dércia Pinto, Ana Rita Barata, Rita Fernandes, Conceição Guedes, Hugo Almeida, Gonçalo Cabecinha, Edna Monteiro, Sandra M. Varandas, Simone Saavedra, Maria José |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Santos, Dércia\"},{\"Person.name\":\"Pinto, Ana Rita\"},{\"Person.name\":\"Barata, Rita\"},{\"Person.name\":\"Fernandes, Conceição\",\"Person.identifier.orcid\":\"0000-0003-2873-501X\"},{\"Person.name\":\"Guedes, Hugo\"},{\"Person.name\":\"Almeida, Gonçalo\"},{\"Person.name\":\"Cabecinha, Edna\"},{\"Person.name\":\"Monteiro, Sandra M.\"},{\"Person.name\":\"Varandas, Simone\"},{\"Person.name\":\"Saavedra, Maria José\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Santos, Dércia Pinto, Ana Rita Barata, Rita Fernandes, Conceição Guedes, Hugo Almeida, Gonçalo Cabecinha, Edna Monteiro, Sandra M. Varandas, Simone Saavedra, Maria José |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-11-14T17:26:41Z |
| datacite.date.embargoed.fl_str_mv | 2025-11-14T17:26:41Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Marine gastropods Coastal areas Antibiotics susceptibility Pathotypes Escherichia coli |
| datacite.titles.title.fl_str_mv | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Santos, Dércia Pinto, Ana Rita Barata, Rita Fernandes, Conceição Guedes, Hugo Almeida, Gonçalo Cabecinha, Edna Monteiro, Sandra M. Varandas, Simone Saavedra, Maria José |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-11-14T17:26:41Z |
| dc.date.embargoed.fl_str_mv | 2025-11-14T17:26:41Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/35081 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Marine gastropods Coastal areas Antibiotics susceptibility Pathotypes Escherichia coli |
| dc.title.fl_str_mv | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Coastal environments have been recognized as key reservoirs for antibiotic-resistant bacteria. The present study evaluated marine gastropods, Phorcus lineatus, as potential bioindicators to assess the spread of antibiotic-resistant bacteria. P. lineatus was sampled in four sites, with different anthropogenic pressures, along the northwest Portuguese coastal area. From these specimens, bacteria were isolated and tested for their antimicrobial susceptibility, followed by their phylogenetic and pathotypic determination. All the Escherichia coli isolates showed resistance to at least one antimicrobial agent. The highest levels of multidrug resistance (25%) were observed in E. coli isolates obtained from SITE 2, which is impacted by the city of Porto and industrial settlements, while nearly 17% of these isolates showed a multiple antibiotic resistance (MAR) index higher than 0.2. Among the isolates, phylogroups A and B2 were the most prevalent, followed by phylogroup B1. The isolates of phylogroup A showed a higher prevalence of antimicrobial resistance. This study offers valuable insights into the antibiotic resistance risks posed to marine ecosystems and underscores the need for microbiological monitoring and the development of effective management strategies. The findings suggest P. lineatus as a potential bioindicator of antibiotic-resistant bacteria in marine environments. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/0865273c-bd9b-4282-92e1-722bb04a4c99/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID |
| id | ipb_21f97d59da001cf91dfd4084529f5d30 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/35081 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/35081 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Santos, Dércia Pinto, Ana Rita Barata, Rita Fernandes, Conceição Fernandes, Conceição https://www.ciencia-id.pt/6514-3D41-4534 6514-3D41-4534 http://orcid.org/0000-0003-2873-501X 0000-0003-2873-501X Guedes, Hugo Almeida, Gonçalo Cabecinha, Edna Monteiro, Sandra M. Varandas, Simone Saavedra, Maria José |
| publishDate | 2025 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
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| spelling | engMDPIengCoastal environments have been recognized as key reservoirs for antibiotic-resistant bacteria. The present study evaluated marine gastropods, Phorcus lineatus, as potential bioindicators to assess the spread of antibiotic-resistant bacteria. P. lineatus was sampled in four sites, with different anthropogenic pressures, along the northwest Portuguese coastal area. From these specimens, bacteria were isolated and tested for their antimicrobial susceptibility, followed by their phylogenetic and pathotypic determination. All the Escherichia coli isolates showed resistance to at least one antimicrobial agent. The highest levels of multidrug resistance (25%) were observed in E. coli isolates obtained from SITE 2, which is impacted by the city of Porto and industrial settlements, while nearly 17% of these isolates showed a multiple antibiotic resistance (MAR) index higher than 0.2. Among the isolates, phylogroups A and B2 were the most prevalent, followed by phylogroup B1. The isolates of phylogroup A showed a higher prevalence of antimicrobial resistance. This study offers valuable insights into the antibiotic resistance risks posed to marine ecosystems and underscores the need for microbiological monitoring and the development of effective management strategies. The findings suggest P. lineatus as a potential bioindicator of antibiotic-resistant bacteria in marine environments.application/pdfengEmergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a BioindicatorSantos, DérciaPinto, Ana RitaBarata, RitaPersonalFernandes, ConceiçãoDSpacehttp://dspace.org/items/d9c26c54-5e10-4ccb-b7f7-528af9eb9950DSpacehttp://dspace.org/items/d9c26c54-5e10-4ccb-b7f7-528af9eb9950FernandesConceiçãoCiência IDhttps://www.ciencia-id.pt6514-3D41-4534ORCIDhttp://orcid.org0000-0003-2873-501XScopus Author IDhttps://www.scopus.com23018336600Guedes, HugoAlmeida, GonçaloCabecinha, EdnaMonteiro, Sandra M.Varandas, SimoneSaavedra, Maria JoséHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2036-7481DOIIsPartOf10.3390/microbiolres160601332025-11-14T17:26:41Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/35081http://purl.org/coar/access_right/c_abf2open accessMarine gastropodsCoastal areasAntibiotics susceptibilityPathotypesEscherichia coli846565 bytesFundação para a Ciência e a TecnologiaInstitute for innovation, capacity building and sustainability of agri-food production6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCenter for the Study of Animal Science6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociate Laboratory for Sustainability and Tecnology in Mountain Regions6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by-nd/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/0865273c-bd9b-4282-92e1-722bb04a4c99/downloadMicrobiology Research166114 |
| spellingShingle | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator Santos, Dércia Marine gastropods Coastal areas Antibiotics susceptibility Pathotypes Escherichia coli |
| status | SINGLETON |
| subject.fl_str_mv | Marine gastropods Coastal areas Antibiotics susceptibility Pathotypes Escherichia coli |
| title | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| title_full | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| title_fullStr | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| title_full_unstemmed | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| title_short | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| title_sort | Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator |
| topic | Marine gastropods Coastal areas Antibiotics susceptibility Pathotypes Escherichia coli |
| topic_facet | Marine gastropods Coastal areas Antibiotics susceptibility Pathotypes Escherichia coli |
| url | http://hdl.handle.net/10198/35081 |
| visible | 1 |