Publicação
COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey
| Resumo: | Honey is a widely consumed food product frequently subjected to adulteration, with the mislabeling of its botanical or geographical origin being one of the most common practices. Determining the entomological origin of honey is particularly challenging but of high relevance for ensuring its authenticity, especially for products with protected designation of origin (PDO) labels. This study presents a novel DNA metabarcoding approach targeting a highly informative 406 bp fragment of the cytochrome c oxidase I (COI) gene to differentiate among the three major mitochondrial lineages (A, M, and C) of honey bees (Apis mellifera L.) native to Europe. The target region was selected based on the calculated fixation index (FST), which is frequently used in Population Genetics as a measure of differentiation between populations. The approach was validated with 11 honey samples of known entomological origin and applied to 44 commercial honeys from 13 countries. The approach demonstrated high sensitivity, accurately identifying the entomological origin of honey, including samples produced by honey bees of varying ancestries, which could not be resolved by previous methods based on real-time PCR coupled with high-resolution melting (PCR-HRM) analysis. The results demonstrate the effectiveness of COI metabarcoding in verifying honey authenticity and highlight the predominance of C-lineage honey bees in the production of commercial honeys from northwestern Europe. This finding suggests a limited presence of the native M-lineage ancestry, underscoring the need for conservation efforts. |
|---|---|
| Autores principais: | Honrado, Mónica |
| Outros Autores: | Quaresma, Andreia; Henriques, Dora; Pinto, M. Alice; Amaral, Joana S. |
| Assunto: | Entomologic origin Apis mellifera MtDNA NA metabarcoding Honey COI gene |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173221835472896 |
|---|---|
| author | Honrado, Mónica |
| author2 | Quaresma, Andreia Henriques, Dora Pinto, M. Alice Amaral, Joana S. |
| author2_role | author author author author |
| author_facet | Honrado, Mónica Quaresma, Andreia Henriques, Dora Pinto, M. Alice Amaral, Joana S. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Honrado, Mónica\",\"Person.identifier.orcid\":\"0000-0002-5126-4693\"},{\"Person.name\":\"Quaresma, Andreia\",\"Person.identifier.orcid\":\"0000-0002-8678-5800\"},{\"Person.name\":\"Henriques, Dora\",\"Person.identifier.orcid\":\"0000-0001-7530-682X\"},{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"},{\"Person.name\":\"Amaral, Joana S.\",\"Person.identifier.orcid\":\"0000-0002-3648-7303\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Honrado, Mónica Quaresma, Andreia Henriques, Dora Pinto, M. Alice Amaral, Joana S. |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-04-07T14:18:15Z |
| datacite.date.embargoed.fl_str_mv | 2025-04-07T14:18:15Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Entomologic origin Apis mellifera MtDNA NA metabarcoding Honey COI gene |
| datacite.titles.title.fl_str_mv | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Honrado, Mónica Quaresma, Andreia Henriques, Dora Pinto, M. Alice Amaral, Joana S. |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-04-07T14:18:15Z |
| dc.date.embargoed.fl_str_mv | 2025-04-07T14:18:15Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/34399 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Entomologic origin Apis mellifera MtDNA NA metabarcoding Honey COI gene |
| dc.title.fl_str_mv | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Honey is a widely consumed food product frequently subjected to adulteration, with the mislabeling of its botanical or geographical origin being one of the most common practices. Determining the entomological origin of honey is particularly challenging but of high relevance for ensuring its authenticity, especially for products with protected designation of origin (PDO) labels. This study presents a novel DNA metabarcoding approach targeting a highly informative 406 bp fragment of the cytochrome c oxidase I (COI) gene to differentiate among the three major mitochondrial lineages (A, M, and C) of honey bees (Apis mellifera L.) native to Europe. The target region was selected based on the calculated fixation index (FST), which is frequently used in Population Genetics as a measure of differentiation between populations. The approach was validated with 11 honey samples of known entomological origin and applied to 44 commercial honeys from 13 countries. The approach demonstrated high sensitivity, accurately identifying the entomological origin of honey, including samples produced by honey bees of varying ancestries, which could not be resolved by previous methods based on real-time PCR coupled with high-resolution melting (PCR-HRM) analysis. The results demonstrate the effectiveness of COI metabarcoding in verifying honey authenticity and highlight the predominance of C-lineage honey bees in the production of commercial honeys from northwestern Europe. This finding suggests a limited presence of the native M-lineage ancestry, underscoring the need for conservation efforts. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/85092375-38c6-4166-aba3-e24c736976c9/download |
| id | ipb_28fcab2aeb903eda097ca30a1e5a53a4 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/34399 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/34399 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Honrado, Mónica Honrado, Mónica https://www.ciencia-id.pt/4712-B40B-4B0E 4712-B40B-4B0E http://orcid.org/0000-0002-5126-4693 0000-0002-5126-4693 Quaresma, Andreia Quaresma, Andreia https://www.ciencia-id.pt/4F1A-4E4A-3F23 4F1A-4E4A-3F23 http://orcid.org/0000-0002-8678-5800 0000-0002-8678-5800 Henriques, Dora Henriques, Dora https://www.ciencia-id.pt/291F-986F-07DA 291F-986F-07DA http://orcid.org/0000-0001-7530-682X 0000-0001-7530-682X Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 Amaral, Joana S. Amaral, Joana S. https://www.ciencia-id.pt/5319-7DE8-BEDA 5319-7DE8-BEDA http://orcid.org/0000-0002-3648-7303 0000-0002-3648-7303 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engMDPIengHoney is a widely consumed food product frequently subjected to adulteration, with the mislabeling of its botanical or geographical origin being one of the most common practices. Determining the entomological origin of honey is particularly challenging but of high relevance for ensuring its authenticity, especially for products with protected designation of origin (PDO) labels. This study presents a novel DNA metabarcoding approach targeting a highly informative 406 bp fragment of the cytochrome c oxidase I (COI) gene to differentiate among the three major mitochondrial lineages (A, M, and C) of honey bees (Apis mellifera L.) native to Europe. The target region was selected based on the calculated fixation index (FST), which is frequently used in Population Genetics as a measure of differentiation between populations. The approach was validated with 11 honey samples of known entomological origin and applied to 44 commercial honeys from 13 countries. The approach demonstrated high sensitivity, accurately identifying the entomological origin of honey, including samples produced by honey bees of varying ancestries, which could not be resolved by previous methods based on real-time PCR coupled with high-resolution melting (PCR-HRM) analysis. The results demonstrate the effectiveness of COI metabarcoding in verifying honey authenticity and highlight the predominance of C-lineage honey bees in the production of commercial honeys from northwestern Europe. This finding suggests a limited presence of the native M-lineage ancestry, underscoring the need for conservation efforts.application/pdfengCOI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European HoneyPersonalHonrado, MónicaDSpacehttp://dspace.org/items/87f8840d-04b1-427a-bca9-d37eadfc0e9bDSpacehttp://dspace.org/items/87f8840d-04b1-427a-bca9-d37eadfc0e9bHonradoMónicaCiência IDhttps://www.ciencia-id.pt4712-B40B-4B0EORCIDhttp://orcid.org0000-0002-5126-4693PersonalQuaresma, AndreiaDSpacehttp://dspace.org/items/d417b0ac-c8ee-473a-a355-820b5b9a3f55DSpacehttp://dspace.org/items/d417b0ac-c8ee-473a-a355-820b5b9a3f55QuaresmaAndreiaCiência IDhttps://www.ciencia-id.pt4F1A-4E4A-3F23ORCIDhttp://orcid.org0000-0002-8678-5800Scopus Author IDhttps://www.scopus.com57119742600PersonalHenriques, DoraDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dHenriquesDoraCiência IDhttps://www.ciencia-id.pt291F-986F-07DAORCIDhttp://orcid.org0000-0001-7530-682XScopus Author IDhttps://www.scopus.com55761737300PersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800PersonalAmaral, Joana S.DSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdDSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdAmaralJoana S.Ciência IDhttps://www.ciencia-id.pt5319-7DE8-BEDAORCIDhttp://orcid.org0000-0002-3648-7303HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2304-8158DOIIsPartOf10.3390/foods140304192025-04-07T14:18:15Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/34399http://purl.org/coar/access_right/c_abf2open accessEntomologic originApis melliferaMtDNANA metabarcodingHoneyCOI gene756595 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/85092375-38c6-4166-aba3-e24c736976c9/downloadFoods143115 |
| spellingShingle | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey Honrado, Mónica Entomologic origin Apis mellifera MtDNA NA metabarcoding Honey COI gene |
| status | SINGLETON |
| subject.fl_str_mv | Entomologic origin Apis mellifera MtDNA NA metabarcoding Honey COI gene |
| title | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| title_full | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| title_fullStr | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| title_full_unstemmed | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| title_short | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| title_sort | COI Metabarcoding as a Novel Approach for Assessing the Honey Bee Source of European Honey |
| topic | Entomologic origin Apis mellifera MtDNA NA metabarcoding Honey COI gene |
| topic_facet | Entomologic origin Apis mellifera MtDNA NA metabarcoding Honey COI gene |
| url | http://hdl.handle.net/10198/34399 |
| visible | 1 |