Publicação
Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies
| Resumo: | Propolis, produced by Apis melliferabees, is composed of several biologically relevant phenolic compounds with known analgesic, anti-inflammatory, antitumor, antioxidant, immunomodulatory, wound healing, and antibacterial effects, having gained significant interest for therapeutic and cosmetic purposes. Niosomes, self-assembled vesicular nanosystems, are highly researched for topical delivery due to providing controlled and sustained release, protecting encapsulated compounds from degradation, improving stability, and having good biocompatibility and biodegradability. This work aimed to develop novel propolis-loaded niosomes with small and homogeneous particle size, high encapsulation efficiency, controlled release, adequate stability, relevant bioactivity, and high safety for topical application, for therapeutic and/or cosmetic purposes. Aided by quality by design (QbD) analysis, niosomes containing Tween 20, Kolliphor RH 40, cetyl alcohol, and/or cholesterol were produced by thin-film hydration followed by extrusion, with small particle size (between 100 and 200 nm), homogeneous distribution (PDI below 0.2), relevant zeta-potential (around -38 mV), good stability both under refrigeration and at room temperature, high encapsulation efficiency (ranging from 78.8 to 87.4%), and a controlled release profile, relevant to ensure prolonged bioactivity at the application site. Adequate concentration-dependent in vitro safety in keratinocytes and fibroblasts (up to 12.5 or 25 mu g/mL) was demonstrated as well, with some niosomal formulations also showing a low irritative potential in a HET-CAM test, and relevant in vitro anti-inflammatory potential (IC50 from 14.90 to 17.89 ng/mL). These novel nanoplatforms, containing a nature-derived hydrophobic compound with various relevant bioactivities, could serve as versatile and advantageous formulations in both pharmaceutical and cosmetic contexts of application. |
|---|---|
| Autores principais: | Pinto, Maria Beatriz |
| Outros Autores: | Pires, Patrícia C.; Correia, Mafalda; Moço, Gabriela; Sousa-Oliveira, Inês; Sousa, Maria João; Calhelha, Ricardo C.; Vilas-Boas, Miguel; Falcão, Soraia; Mazzola, Priscila Gava; Veiga, Francisco; Paiva-Santos, Ana Cláudia |
| Assunto: | Anti-inflammatory Controlled release Niosomes Propolis QbD Topical delivery |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173101364576256 |
|---|---|
| author | Pinto, Maria Beatriz |
| author2 | Pires, Patrícia C. Correia, Mafalda Moço, Gabriela Sousa-Oliveira, Inês Sousa, Maria João Calhelha, Ricardo C. Vilas-Boas, Miguel Falcão, Soraia Mazzola, Priscila Gava Veiga, Francisco Paiva-Santos, Ana Cláudia |
| author2_role | author author author author author author author author author author author |
| author_facet | Pinto, Maria Beatriz Pires, Patrícia C. Correia, Mafalda Moço, Gabriela Sousa-Oliveira, Inês Sousa, Maria João Calhelha, Ricardo C. Vilas-Boas, Miguel Falcão, Soraia Mazzola, Priscila Gava Veiga, Francisco Paiva-Santos, Ana Cláudia |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pinto, Maria Beatriz\"},{\"Person.name\":\"Pires, Patrícia C.\"},{\"Person.name\":\"Correia, Mafalda\"},{\"Person.name\":\"Moço, Gabriela\"},{\"Person.name\":\"Sousa-Oliveira, Inês\"},{\"Person.name\":\"Sousa, Maria João\",\"Person.identifier.orcid\":\"0000-0002-9946-4926\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Vilas-Boas, Miguel\",\"Person.identifier.orcid\":\"0000-0002-8665-5280\"},{\"Person.name\":\"Falcão, Soraia\",\"Person.identifier.orcid\":\"0000-0003-3735-6951\"},{\"Person.name\":\"Mazzola, Priscila Gava\"},{\"Person.name\":\"Veiga, Francisco\"},{\"Person.name\":\"Paiva-Santos, Ana Cláudia\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pinto, Maria Beatriz Pires, Patrícia C. Correia, Mafalda Moço, Gabriela Sousa-Oliveira, Inês Sousa, Maria João Calhelha, Ricardo C. Vilas-Boas, Miguel Falcão, Soraia Mazzola, Priscila Gava Veiga, Francisco Paiva-Santos, Ana Cláudia |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-01-23T16:46:02Z |
| datacite.date.embargoed.fl_str_mv | 2026-01-23T16:46:02Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Anti-inflammatory Controlled release Niosomes Propolis QbD Topical delivery |
| datacite.titles.title.fl_str_mv | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pinto, Maria Beatriz Pires, Patrícia C. Correia, Mafalda Moço, Gabriela Sousa-Oliveira, Inês Sousa, Maria João Calhelha, Ricardo C. Vilas-Boas, Miguel Falcão, Soraia Mazzola, Priscila Gava Veiga, Francisco Paiva-Santos, Ana Cláudia |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-01-23T16:46:02Z |
| dc.date.embargoed.fl_str_mv | 2026-01-23T16:46:02Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/35595 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | American Chemical Society |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Anti-inflammatory Controlled release Niosomes Propolis QbD Topical delivery |
| dc.title.fl_str_mv | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Propolis, produced by Apis melliferabees, is composed of several biologically relevant phenolic compounds with known analgesic, anti-inflammatory, antitumor, antioxidant, immunomodulatory, wound healing, and antibacterial effects, having gained significant interest for therapeutic and cosmetic purposes. Niosomes, self-assembled vesicular nanosystems, are highly researched for topical delivery due to providing controlled and sustained release, protecting encapsulated compounds from degradation, improving stability, and having good biocompatibility and biodegradability. This work aimed to develop novel propolis-loaded niosomes with small and homogeneous particle size, high encapsulation efficiency, controlled release, adequate stability, relevant bioactivity, and high safety for topical application, for therapeutic and/or cosmetic purposes. Aided by quality by design (QbD) analysis, niosomes containing Tween 20, Kolliphor RH 40, cetyl alcohol, and/or cholesterol were produced by thin-film hydration followed by extrusion, with small particle size (between 100 and 200 nm), homogeneous distribution (PDI below 0.2), relevant zeta-potential (around -38 mV), good stability both under refrigeration and at room temperature, high encapsulation efficiency (ranging from 78.8 to 87.4%), and a controlled release profile, relevant to ensure prolonged bioactivity at the application site. Adequate concentration-dependent in vitro safety in keratinocytes and fibroblasts (up to 12.5 or 25 mu g/mL) was demonstrated as well, with some niosomal formulations also showing a low irritative potential in a HET-CAM test, and relevant in vitro anti-inflammatory potential (IC50 from 14.90 to 17.89 ng/mL). These novel nanoplatforms, containing a nature-derived hydrophobic compound with various relevant bioactivities, could serve as versatile and advantageous formulations in both pharmaceutical and cosmetic contexts of application. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/8813dfb6-1c13-4ada-90f4-498c99878f22/download |
| id | ipb_2bc2f1fde0ced1b7730bfa3d7a8e5bd1 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/35595 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/35595 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pinto, Maria Beatriz Pires, Patrícia C. Correia, Mafalda Moço, Gabriela Sousa-Oliveira, Inês Sousa, Maria João Sousa, Maria João https://www.ciencia-id.pt/D91E-3571-3979 D91E-3571-3979 http://orcid.org/0000-0002-9946-4926 0000-0002-9946-4926 Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Vilas-Boas, Miguel Vilas-Boas, Miguel https://www.ciencia-id.pt/A918-C6FF-81A4 A918-C6FF-81A4 http://orcid.org/0000-0002-8665-5280 0000-0002-8665-5280 Falcão, Soraia Falcão, Soraia https://www.ciencia-id.pt/D41A-6BED-4CBB D41A-6BED-4CBB http://orcid.org/0000-0003-3735-6951 0000-0003-3735-6951 Mazzola, Priscila Gava Veiga, Francisco Paiva-Santos, Ana Cláudia |
| publishDate | 2025 |
| publisher.none.fl_str_mv | American Chemical Society |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engAmerican Chemical SocietyengPropolis, produced by Apis melliferabees, is composed of several biologically relevant phenolic compounds with known analgesic, anti-inflammatory, antitumor, antioxidant, immunomodulatory, wound healing, and antibacterial effects, having gained significant interest for therapeutic and cosmetic purposes. Niosomes, self-assembled vesicular nanosystems, are highly researched for topical delivery due to providing controlled and sustained release, protecting encapsulated compounds from degradation, improving stability, and having good biocompatibility and biodegradability. This work aimed to develop novel propolis-loaded niosomes with small and homogeneous particle size, high encapsulation efficiency, controlled release, adequate stability, relevant bioactivity, and high safety for topical application, for therapeutic and/or cosmetic purposes. Aided by quality by design (QbD) analysis, niosomes containing Tween 20, Kolliphor RH 40, cetyl alcohol, and/or cholesterol were produced by thin-film hydration followed by extrusion, with small particle size (between 100 and 200 nm), homogeneous distribution (PDI below 0.2), relevant zeta-potential (around -38 mV), good stability both under refrigeration and at room temperature, high encapsulation efficiency (ranging from 78.8 to 87.4%), and a controlled release profile, relevant to ensure prolonged bioactivity at the application site. Adequate concentration-dependent in vitro safety in keratinocytes and fibroblasts (up to 12.5 or 25 mu g/mL) was demonstrated as well, with some niosomal formulations also showing a low irritative potential in a HET-CAM test, and relevant in vitro anti-inflammatory potential (IC50 from 14.90 to 17.89 ng/mL). These novel nanoplatforms, containing a nature-derived hydrophobic compound with various relevant bioactivities, could serve as versatile and advantageous formulations in both pharmaceutical and cosmetic contexts of application.application/pdfengPropolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity StudiesPinto, Maria BeatrizPires, Patrícia C.Correia, MafaldaMoço, GabrielaSousa-Oliveira, InêsPersonalSousa, Maria JoãoDSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0DSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0SousaMaria JoãoCiência IDhttps://www.ciencia-id.ptD91E-3571-3979ORCIDhttp://orcid.org0000-0002-9946-4926Scopus Author IDhttps://www.scopus.com55230282700PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalVilas-Boas, MiguelDSpacehttp://dspace.org/items/f58280c8-298b-4e4c-9f7c-4517300d3382DSpacehttp://dspace.org/items/f58280c8-298b-4e4c-9f7c-4517300d3382Vilas-BoasMiguelCiência IDhttps://www.ciencia-id.ptA918-C6FF-81A4ORCIDhttp://orcid.org0000-0002-8665-5280Researcher IDhttps://www.researcherid.comI-5949-2013Scopus Author IDhttps://www.scopus.com6602648497PersonalFalcão, SoraiaDSpacehttp://dspace.org/items/5746ab3e-8ffe-4e26-9a41-8392cf031f28DSpacehttp://dspace.org/items/5746ab3e-8ffe-4e26-9a41-8392cf031f28FalcãoSoraiaCiência IDhttps://www.ciencia-id.ptD41A-6BED-4CBBORCIDhttp://orcid.org0000-0003-3735-6951Scopus Author IDhttps://www.scopus.com14832459200Mazzola, Priscila GavaVeiga, FranciscoPaiva-Santos, Ana CláudiaHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2575-9108ISSNIsPartOf2575-9108DOIIsPartOf10.1021/acsptsci.5c002532026-01-23T16:46:02Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/35595http://purl.org/coar/access_right/c_abf2open accessAnti-inflammatoryControlled releaseNiosomesPropolisQbDTopical delivery14326158 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by-nd/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/8813dfb6-1c13-4ada-90f4-498c99878f22/downloadACS Pharmacology & Translational Science8722202246 |
| spellingShingle | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies Pinto, Maria Beatriz Anti-inflammatory Controlled release Niosomes Propolis QbD Topical delivery |
| status | SINGLETON |
| subject.fl_str_mv | Anti-inflammatory Controlled release Niosomes Propolis QbD Topical delivery |
| title | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| title_full | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| title_fullStr | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| title_full_unstemmed | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| title_short | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| title_sort | Propolis-Loaded Niosomes for Dermopharmaceutic and Cosmetic Applications: Development, Stability, Safety, and In Vitro Bioactivity Studies |
| topic | Anti-inflammatory Controlled release Niosomes Propolis QbD Topical delivery |
| topic_facet | Anti-inflammatory Controlled release Niosomes Propolis QbD Topical delivery |
| url | http://hdl.handle.net/10198/35595 |
| visible | 1 |