Publicação
Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal
| Resumo: | Bee bread (BB) is a fermented mixture of plant pollen, honey, and bee saliva that worker bees use as food for larvae, and for young bees to produce royal jelly. In the present study, five BB samples, collected from Apis mellifera iberiensis hives located in different apiaries near Bragança, in the northeast region of Portugal, and one BB commercial sample were characterized by high-performance liquid chromatography coupled to a diode array detector and electrospray mass spectrometry (HPLC-DAD-ESI/MS) in terms of phenolic compounds, such as flavonoid glycoside derivatives. Furthermore, the samples were screened, using in vitro assays, against different human tumor cell lines, MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), HeLa (cervical carcinoma) and HepG2 (hepatocellular carcinoma), and also against non-tumor liver cells (porcine liver cells, PLP2). The main phenolic compounds found were flavonol derivatives, mainly quercetin, kaempferol, myricetin, isorhamnetin and herbacetrin glycoside derivatives. Thirty-two compounds were identified in the six BB samples, presenting BB1 and BB3 with the highest contents (6802 and 6480 µg/g extract, respectively) and the highest number of identified compounds. Two isorhamnetin glycoside derivatives, isrohamnetin-O-hexosyl-O-rutinoside and isorhamnetin-O-pentosyl-hexoside, were the most abundant compounds present in BB1; on the other hand, quercetin-3-O-rhamnoside was the most abundant flavonol in BB3. However, it was not possible to establish a correlation between the flavonoids and the observed low to moderate cytotoxicity (ranging from > 400 to 68 µg/mL), in which HeLa and NCI-H460 cell lines were the most susceptible to the inhibition. To the authors' knowledge, this is the first report characterizing glycosidic flavonoids in BB samples, contributing to the chemical knowledge of this less explored bee product. |
|---|---|
| Autores principais: | Sobral, Filipa |
| Outros Autores: | Calhelha, Ricardo C.; Barros, Lillian; Dueñas, Montserrat; Tomás, Andreia; Santos-Buelga, Celestino; Vilas-Boas, Miguel; Ferreira, Isabel C.F.R. |
| Assunto: | Apis mellifera iberiensis Bee bread Cytotoxicity HPLC-DAD-ESI/MS Phenolic compounds |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850889758900224 |
|---|---|
| author | Sobral, Filipa |
| author2 | Calhelha, Ricardo C. Barros, Lillian Dueñas, Montserrat Tomás, Andreia Santos-Buelga, Celestino Vilas-Boas, Miguel Ferreira, Isabel C.F.R. |
| author2_role | author author author author author author author |
| author_facet | Sobral, Filipa Calhelha, Ricardo C. Barros, Lillian Dueñas, Montserrat Tomás, Andreia Santos-Buelga, Celestino Vilas-Boas, Miguel Ferreira, Isabel C.F.R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Sobral, Filipa\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Dueñas, Montserrat\"},{\"Person.name\":\"Tomás, Andreia\"},{\"Person.name\":\"Santos-Buelga, Celestino\"},{\"Person.name\":\"Vilas-Boas, Miguel\",\"Person.identifier.orcid\":\"0000-0002-8665-5280\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Sobral, Filipa Calhelha, Ricardo C. Barros, Lillian Dueñas, Montserrat Tomás, Andreia Santos-Buelga, Celestino Vilas-Boas, Miguel Ferreira, Isabel C.F.R. |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-25T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-25T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Apis mellifera iberiensis Bee bread Cytotoxicity HPLC-DAD-ESI/MS Phenolic compounds |
| datacite.titles.title.fl_str_mv | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Sobral, Filipa Calhelha, Ricardo C. Barros, Lillian Dueñas, Montserrat Tomás, Andreia Santos-Buelga, Celestino Vilas-Boas, Miguel Ferreira, Isabel C.F.R. |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-25T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-25T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/15312 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Apis mellifera iberiensis Bee bread Cytotoxicity HPLC-DAD-ESI/MS Phenolic compounds |
| dc.title.fl_str_mv | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bee bread (BB) is a fermented mixture of plant pollen, honey, and bee saliva that worker bees use as food for larvae, and for young bees to produce royal jelly. In the present study, five BB samples, collected from Apis mellifera iberiensis hives located in different apiaries near Bragança, in the northeast region of Portugal, and one BB commercial sample were characterized by high-performance liquid chromatography coupled to a diode array detector and electrospray mass spectrometry (HPLC-DAD-ESI/MS) in terms of phenolic compounds, such as flavonoid glycoside derivatives. Furthermore, the samples were screened, using in vitro assays, against different human tumor cell lines, MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), HeLa (cervical carcinoma) and HepG2 (hepatocellular carcinoma), and also against non-tumor liver cells (porcine liver cells, PLP2). The main phenolic compounds found were flavonol derivatives, mainly quercetin, kaempferol, myricetin, isorhamnetin and herbacetrin glycoside derivatives. Thirty-two compounds were identified in the six BB samples, presenting BB1 and BB3 with the highest contents (6802 and 6480 µg/g extract, respectively) and the highest number of identified compounds. Two isorhamnetin glycoside derivatives, isrohamnetin-O-hexosyl-O-rutinoside and isorhamnetin-O-pentosyl-hexoside, were the most abundant compounds present in BB1; on the other hand, quercetin-3-O-rhamnoside was the most abundant flavonol in BB3. However, it was not possible to establish a correlation between the flavonoids and the observed low to moderate cytotoxicity (ranging from > 400 to 68 µg/mL), in which HeLa and NCI-H460 cell lines were the most susceptible to the inhibition. To the authors' knowledge, this is the first report characterizing glycosidic flavonoids in BB samples, contributing to the chemical knowledge of this less explored bee product. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/8afb1dfb-ed7b-4abb-8069-0aa56ae0e8f5/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | SFRH/BPD/107855/2015 UID/AGR/00690/2013 |
| funding.name_str_mv | 6817 - DCRRNI ID |
| funding_str_mv | SFRH/BPD/107855/2015 info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F107855%2F2015/PT UID/AGR/00690/2013 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2013/PT |
| id | ipb_2c7dd0f8cdae4db25474d439a709dc44 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/15312 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/15312 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Sobral, Filipa Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Dueñas, Montserrat Tomás, Andreia Tomás, Andreia https://www.ciencia-id.pt/1C1B-8981-E49E 1C1B-8981-E49E Santos-Buelga, Celestino Vilas-Boas, Miguel Vilas-Boas, Miguel https://www.ciencia-id.pt/A918-C6FF-81A4 A918-C6FF-81A4 http://orcid.org/0000-0002-8665-5280 0000-0002-8665-5280 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 |
| publishDate | 2017 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engpt_PTBee bread (BB) is a fermented mixture of plant pollen, honey, and bee saliva that worker bees use as food for larvae, and for young bees to produce royal jelly. In the present study, five BB samples, collected from Apis mellifera iberiensis hives located in different apiaries near Bragança, in the northeast region of Portugal, and one BB commercial sample were characterized by high-performance liquid chromatography coupled to a diode array detector and electrospray mass spectrometry (HPLC-DAD-ESI/MS) in terms of phenolic compounds, such as flavonoid glycoside derivatives. Furthermore, the samples were screened, using in vitro assays, against different human tumor cell lines, MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), HeLa (cervical carcinoma) and HepG2 (hepatocellular carcinoma), and also against non-tumor liver cells (porcine liver cells, PLP2). The main phenolic compounds found were flavonol derivatives, mainly quercetin, kaempferol, myricetin, isorhamnetin and herbacetrin glycoside derivatives. Thirty-two compounds were identified in the six BB samples, presenting BB1 and BB3 with the highest contents (6802 and 6480 µg/g extract, respectively) and the highest number of identified compounds. Two isorhamnetin glycoside derivatives, isrohamnetin-O-hexosyl-O-rutinoside and isorhamnetin-O-pentosyl-hexoside, were the most abundant compounds present in BB1; on the other hand, quercetin-3-O-rhamnoside was the most abundant flavonol in BB3. However, it was not possible to establish a correlation between the flavonoids and the observed low to moderate cytotoxicity (ranging from > 400 to 68 µg/mL), in which HeLa and NCI-H460 cell lines were the most susceptible to the inhibition. To the authors' knowledge, this is the first report characterizing glycosidic flavonoids in BB samples, contributing to the chemical knowledge of this less explored bee product.application/pdfen_ENFlavonoid composition and antitumor activity of bee bread collected in Northeast PortugalSobral, FilipaPersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801Dueñas, MontserratPersonalTomás, AndreiaDSpacehttp://dspace.org/items/9cb298ef-31c6-4761-8842-b842e46dca0fDSpacehttp://dspace.org/items/9cb298ef-31c6-4761-8842-b842e46dca0fTomásAndreiaCiência IDhttps://www.ciencia-id.pt1C1B-8981-E49ESantos-Buelga, CelestinoPersonalVilas-Boas, MiguelDSpacehttp://dspace.org/items/f58280c8-298b-4e4c-9f7c-4517300d3382DSpacehttp://dspace.org/items/f58280c8-298b-4e4c-9f7c-4517300d3382Vilas-BoasMiguelCiência IDhttps://www.ciencia-id.ptA918-C6FF-81A4ORCIDhttp://orcid.org0000-0002-8665-5280Researcher IDhttps://www.researcherid.comI-5949-2013Scopus Author IDhttps://www.scopus.com6602648497PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf1420-3049DOIIsPartOf10.3390/molecules220202482018-01-25T10:00:00Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/15312http://purl.org/coar/access_right/c_abf2open accessApis mellifera iberiensisBee breadCytotoxicityHPLC-DAD-ESI/MSPhenolic compounds919397 bytesFundação para a Ciência e a TecnologiaProviding industry and consumers with new alternative sources of natural additives from plant origin in the classes of preservatives and colouring agents: extraction, purification, stabilization and product developmentinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F107855%2F2015/PTSFRH/BPD/107855/2015Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Centreinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2013/PTUID/AGR/00690/20136817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/8afb1dfb-ed7b-4abb-8069-0aa56ae0e8f5/download |
| spellingShingle | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal Sobral, Filipa Apis mellifera iberiensis Bee bread Cytotoxicity HPLC-DAD-ESI/MS Phenolic compounds |
| subject.fl_str_mv | Apis mellifera iberiensis Bee bread Cytotoxicity HPLC-DAD-ESI/MS Phenolic compounds |
| title | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| title_full | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| title_fullStr | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| title_full_unstemmed | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| title_short | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| title_sort | Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal |
| topic | Apis mellifera iberiensis Bee bread Cytotoxicity HPLC-DAD-ESI/MS Phenolic compounds |
| topic_facet | Apis mellifera iberiensis Bee bread Cytotoxicity HPLC-DAD-ESI/MS Phenolic compounds |
| url | http://hdl.handle.net/10198/15312 |
| visible | 1 |
Atividades financiadas
Carregando projetos financiados...