Publicação
Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore
| Resumo: | Lentinus tigrinus is a wood-decay fungus known for its nutritional, culinary, and medicinal benefits. It contains bioactive compounds like polyphenols, terpenes, and flavonoids that exhibit antioxidant, anti-microbial, and anti-inflammatory effects. These natural antioxidants are increasingly recognized for their potential to prevent oxidative damage linked to aging and chronic diseases. This study investigates the antioxidant activity of hydroalcoholic extracts obtained from L. tigrinus sporophores cultivated on hazelnut shells (Lt1_HS), a waste material rich in phenolic compounds, and sporophores cultivated on sawdust (Lt1_S). Bioactivity tests, including DPPH, TBARS, MTT, and DCFHDA assays, were performed to assess the hydroalcoholic extracts’ efficiency. The results showed that all the extracts contained various bioactive compounds, primarily polyphenols. Notably, the caffeoylquinic acids present in HS and Lt1_HS are linked to anti-peroxidant effects. Biological analyses demonstrated that the Lt1_HS extract has higher anti-peroxidant activity (IC50 0.77 ± 0.01 mg/mL) compared to Lt1_S (IC50 1.36 ± 0.01 mg/mL) and reduces the accumulation of reactive oxygen species in HaCaT cells by 80%. However, the specific bioactive compounds responsible for these antioxidant effects are still unclear, and further analysis will be conducted. Additionally, this study promotes recycling hazelnut shells as a valuable substrate for fungal cultivation, supporting sustainable waste management. |
|---|---|
| Autores principais: | Desiderio, Anthea |
| Outros Autores: | Pedrosa, Mariana C.; Heleno, Sandrina A.; Carocho, Márcio; Rodrigues, Daniele Bobrowski; Buratti, Simone; Soffientini, Irene; Ratto, Daniela; Savino, Elena; Rossi, Paola |
| Assunto: | Medicinal mushrooms Biological effects Cultivation Waste substrate Bioactive compounds |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851076323639296 |
|---|---|
| author | Desiderio, Anthea |
| author2 | Pedrosa, Mariana C. Heleno, Sandrina A. Carocho, Márcio Rodrigues, Daniele Bobrowski Buratti, Simone Soffientini, Irene Ratto, Daniela Savino, Elena Rossi, Paola |
| author2_role | author author author author author author author author author |
| author_facet | Desiderio, Anthea Pedrosa, Mariana C. Heleno, Sandrina A. Carocho, Márcio Rodrigues, Daniele Bobrowski Buratti, Simone Soffientini, Irene Ratto, Daniela Savino, Elena Rossi, Paola |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Desiderio, Anthea\"},{\"Person.name\":\"Pedrosa, Mariana C.\",\"Person.identifier.orcid\":\"0000-0002-2057-0639\"},{\"Person.name\":\"Heleno, Sandrina A.\",\"Person.identifier.orcid\":\"0000-0001-7224-1098\"},{\"Person.name\":\"Carocho, Márcio\",\"Person.identifier.orcid\":\"0000-0002-8978-4547\"},{\"Person.name\":\"Rodrigues, Daniele Bobrowski\",\"Person.identifier.orcid\":\"0000-0003-0260-5751\"},{\"Person.name\":\"Buratti, Simone\"},{\"Person.name\":\"Soffientini, Irene\"},{\"Person.name\":\"Ratto, Daniela\"},{\"Person.name\":\"Savino, Elena\"},{\"Person.name\":\"Rossi, Paola\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Desiderio, Anthea Pedrosa, Mariana C. Heleno, Sandrina A. Carocho, Márcio Rodrigues, Daniele Bobrowski Buratti, Simone Soffientini, Irene Ratto, Daniela Savino, Elena Rossi, Paola |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-02-10T11:13:40Z |
| datacite.date.embargoed.fl_str_mv | 2025-02-10T11:13:40Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Medicinal mushrooms Biological effects Cultivation Waste substrate Bioactive compounds |
| datacite.titles.title.fl_str_mv | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Desiderio, Anthea Pedrosa, Mariana C. Heleno, Sandrina A. Carocho, Márcio Rodrigues, Daniele Bobrowski Buratti, Simone Soffientini, Irene Ratto, Daniela Savino, Elena Rossi, Paola |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-02-10T11:13:40Z |
| dc.date.embargoed.fl_str_mv | 2025-02-10T11:13:40Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/31139 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Medicinal mushrooms Biological effects Cultivation Waste substrate Bioactive compounds |
| dc.title.fl_str_mv | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Lentinus tigrinus is a wood-decay fungus known for its nutritional, culinary, and medicinal benefits. It contains bioactive compounds like polyphenols, terpenes, and flavonoids that exhibit antioxidant, anti-microbial, and anti-inflammatory effects. These natural antioxidants are increasingly recognized for their potential to prevent oxidative damage linked to aging and chronic diseases. This study investigates the antioxidant activity of hydroalcoholic extracts obtained from L. tigrinus sporophores cultivated on hazelnut shells (Lt1_HS), a waste material rich in phenolic compounds, and sporophores cultivated on sawdust (Lt1_S). Bioactivity tests, including DPPH, TBARS, MTT, and DCFHDA assays, were performed to assess the hydroalcoholic extracts’ efficiency. The results showed that all the extracts contained various bioactive compounds, primarily polyphenols. Notably, the caffeoylquinic acids present in HS and Lt1_HS are linked to anti-peroxidant effects. Biological analyses demonstrated that the Lt1_HS extract has higher anti-peroxidant activity (IC50 0.77 ± 0.01 mg/mL) compared to Lt1_S (IC50 1.36 ± 0.01 mg/mL) and reduces the accumulation of reactive oxygen species in HaCaT cells by 80%. However, the specific bioactive compounds responsible for these antioxidant effects are still unclear, and further analysis will be conducted. Additionally, this study promotes recycling hazelnut shells as a valuable substrate for fungal cultivation, supporting sustainable waste management. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/730147c2-da3b-46de-a224-1717440c9822/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID CEEC IND 2018 |
| id | ipb_2e6b13dd6db5d982ef6127c8dabeb607 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/31139 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/31139 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Desiderio, Anthea Pedrosa, Mariana C. Pedrosa, Mariana C. https://www.ciencia-id.pt/971F-DA77-195D 971F-DA77-195D http://orcid.org/0000-0002-2057-0639 0000-0002-2057-0639 Heleno, Sandrina A. Heleno, Sandrina A. https://www.ciencia-id.pt/8B18-3095-6FC9 8B18-3095-6FC9 http://orcid.org/0000-0001-7224-1098 0000-0001-7224-1098 Carocho, Márcio Carocho, Márcio https://www.ciencia-id.pt/5614-005B-5CF1 5614-005B-5CF1 http://orcid.org/0000-0002-8978-4547 0000-0002-8978-4547 Rodrigues, Daniele Bobrowski Rodrigues, Daniele Bobrowski https://www.ciencia-id.pt/A91B-FE1D-B275 A91B-FE1D-B275 http://orcid.org/0000-0003-0260-5751 0000-0003-0260-5751 Buratti, Simone Soffientini, Irene Ratto, Daniela Savino, Elena Rossi, Paola |
| publishDate | 2025 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engMDPIpt_PTLentinus tigrinus is a wood-decay fungus known for its nutritional, culinary, and medicinal benefits. It contains bioactive compounds like polyphenols, terpenes, and flavonoids that exhibit antioxidant, anti-microbial, and anti-inflammatory effects. These natural antioxidants are increasingly recognized for their potential to prevent oxidative damage linked to aging and chronic diseases. This study investigates the antioxidant activity of hydroalcoholic extracts obtained from L. tigrinus sporophores cultivated on hazelnut shells (Lt1_HS), a waste material rich in phenolic compounds, and sporophores cultivated on sawdust (Lt1_S). Bioactivity tests, including DPPH, TBARS, MTT, and DCFHDA assays, were performed to assess the hydroalcoholic extracts’ efficiency. The results showed that all the extracts contained various bioactive compounds, primarily polyphenols. Notably, the caffeoylquinic acids present in HS and Lt1_HS are linked to anti-peroxidant effects. Biological analyses demonstrated that the Lt1_HS extract has higher anti-peroxidant activity (IC50 0.77 ± 0.01 mg/mL) compared to Lt1_S (IC50 1.36 ± 0.01 mg/mL) and reduces the accumulation of reactive oxygen species in HaCaT cells by 80%. However, the specific bioactive compounds responsible for these antioxidant effects are still unclear, and further analysis will be conducted. Additionally, this study promotes recycling hazelnut shells as a valuable substrate for fungal cultivation, supporting sustainable waste management.application/pdfpt_PTBio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus SporophoreDesiderio, AntheaPersonalPedrosa, Mariana C.DSpacehttp://dspace.org/items/20df969b-827a-4c9f-be8f-d727edd1a9caDSpacehttp://dspace.org/items/20df969b-827a-4c9f-be8f-d727edd1a9caPedrosaMariana C.Ciência IDhttps://www.ciencia-id.pt971F-DA77-195DORCIDhttp://orcid.org0000-0002-2057-0639PersonalHeleno, Sandrina A.DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676HelenoSandrina A.Ciência IDhttps://www.ciencia-id.pt8B18-3095-6FC9ORCIDhttp://orcid.org0000-0001-7224-1098Researcher IDhttps://www.researcherid.comL-5951-2014Scopus Author IDhttps://www.scopus.com30067731800PersonalCarocho, MárcioDSpacehttp://dspace.org/items/46b39e1f-2674-4be1-a96a-c2e5792e6979DSpacehttp://dspace.org/items/46b39e1f-2674-4be1-a96a-c2e5792e6979CarochoMárcioCiência IDhttps://www.ciencia-id.pt5614-005B-5CF1ORCIDhttp://orcid.org0000-0002-8978-4547Scopus Author IDhttps://www.scopus.com52463229800PersonalRodrigues, Daniele BobrowskiDSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3DSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3RodriguesDaniele BobrowskiCiência IDhttps://www.ciencia-id.ptA91B-FE1D-B275ORCIDhttp://orcid.org0000-0003-0260-5751Buratti, SimoneSoffientini, IreneRatto, DanielaSavino, ElenaRossi, PaolaHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2077-0472DOIIsPartOf10.3390/agriculture150201782025-02-10T11:13:40Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/31139http://purl.org/coar/access_right/c_abf2open accessMedicinal mushroomsBiological effectsCultivationWaste substrateBioactive compounds2181783 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociate Laboratory for Sustainability and Tecnology in Mountain Regions6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaNot AvailableCEEC IND 2018Crossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/730147c2-da3b-46de-a224-1717440c9822/downloadAgriculture152120 |
| spellingShingle | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore Desiderio, Anthea Medicinal mushrooms Biological effects Cultivation Waste substrate Bioactive compounds |
| subject.fl_str_mv | Medicinal mushrooms Biological effects Cultivation Waste substrate Bioactive compounds |
| title | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| title_full | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| title_fullStr | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| title_full_unstemmed | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| title_short | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| title_sort | Bio-Recycling Hazelnut Shells to Improve Antioxidant Properties of Lentinus tigrinus Sporophore |
| topic | Medicinal mushrooms Biological effects Cultivation Waste substrate Bioactive compounds |
| topic_facet | Medicinal mushrooms Biological effects Cultivation Waste substrate Bioactive compounds |
| url | http://hdl.handle.net/10198/31139 |
| visible | 1 |