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Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi

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Resumo:Mycotoxins are secondary metabolites produced by moulds and are an important world-wide food safety concern. Among the most relevant mycotoxigenic producer fungi are some Aspergillus species in particular those belonging to the Aspergillus section Flavi. These are known to produce the highly carcinogenic aflatoxins in agricultural commodities. Due to its impact in animal and human health, these species are among the most intenSively studied ones, being well known producers of aflatoxins (AFB1 , AFB2; AFG1 and AFG2) and cyciopiazonic acid (CPA). Aflatoxins are mainly produced by some strains of Aspergillus flavus and Aspergillus nomius and by most, if not all, strains of Aspergillus parasiticus. On the other hand, cyclopiazonic acid, which naturally occurs in a large variety of crop products as a co-contaminant with aflatoxins, is mainly produced by Aspergillus flavus strains. Together they have been shown to cause health problems in animals and humans, resulting in important economic losses. The production of CPA by Aspergillus section Flavi may also be routinely used for identification purposes since A. parasiticus, A. flavus and A. nomius, exhibit different mycotoxin profiles. The detection and quantification of both these mycotoxins is usually done separately by HPLC with UV detection for CPA and fluorescence detection after post-column derivatization for aflatoxins. There isn't a chromatographic method available to detect simultaneously CPA and the main four aflatoxins.
Autores principais:Soares, Célia
Outros Autores:Rodrigues, Paula; Freitas-Silva, Otniel; Abrunhosa, Luís; Venâncio, Armando
Assunto:Multimycotoxin detection Filamentous fungi
Ano:2010
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso a metadados
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Soares, Célia
author2 Rodrigues, Paula
Freitas-Silva, Otniel
Abrunhosa, Luís
Venâncio, Armando
author2_role author
author
author
author
author_facet Soares, Célia
Rodrigues, Paula
Freitas-Silva, Otniel
Abrunhosa, Luís
Venâncio, Armando
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Soares, Célia\"},{\"Person.name\":\"Rodrigues, Paula\",\"Person.identifier.orcid\":\"0000-0002-3789-2730\"},{\"Person.name\":\"Freitas-Silva, Otniel\"},{\"Person.name\":\"Abrunhosa, Luís\"},{\"Person.name\":\"Venâncio, Armando\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Soares, Célia
Rodrigues, Paula
Freitas-Silva, Otniel
Abrunhosa, Luís
Venâncio, Armando
datacite.date.Accepted.fl_str_mv 2010-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2011-05-12T09:40:48Z
datacite.date.embargoed.fl_str_mv 2011-05-12T09:40:48Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_14cb
datacite.subjects.subject.fl_str_mv Multimycotoxin detection
Filamentous fungi
datacite.titles.title.fl_str_mv Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Soares, Célia
Rodrigues, Paula
Freitas-Silva, Otniel
Abrunhosa, Luís
Venâncio, Armando
dc.date.Accepted.fl_str_mv 2010-01-01T00:00:00Z
dc.date.available.fl_str_mv 2011-05-12T09:40:48Z
dc.date.embargoed.fl_str_mv 2011-05-12T09:40:48Z
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/4240
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
dc.subject.none.fl_str_mv Multimycotoxin detection
Filamentous fungi
dc.title.fl_str_mv Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description Mycotoxins are secondary metabolites produced by moulds and are an important world-wide food safety concern. Among the most relevant mycotoxigenic producer fungi are some Aspergillus species in particular those belonging to the Aspergillus section Flavi. These are known to produce the highly carcinogenic aflatoxins in agricultural commodities. Due to its impact in animal and human health, these species are among the most intenSively studied ones, being well known producers of aflatoxins (AFB1 , AFB2; AFG1 and AFG2) and cyciopiazonic acid (CPA). Aflatoxins are mainly produced by some strains of Aspergillus flavus and Aspergillus nomius and by most, if not all, strains of Aspergillus parasiticus. On the other hand, cyclopiazonic acid, which naturally occurs in a large variety of crop products as a co-contaminant with aflatoxins, is mainly produced by Aspergillus flavus strains. Together they have been shown to cause health problems in animals and humans, resulting in important economic losses. The production of CPA by Aspergillus section Flavi may also be routinely used for identification purposes since A. parasiticus, A. flavus and A. nomius, exhibit different mycotoxin profiles. The detection and quantification of both these mycotoxins is usually done separately by HPLC with UV detection for CPA and fluorescence detection after post-column derivatization for aflatoxins. There isn't a chromatographic method available to detect simultaneously CPA and the main four aflatoxins.
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person_str_mv Soares, Célia
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Freitas-Silva, Otniel
Abrunhosa, Luís
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spelling engporMycotoxins are secondary metabolites produced by moulds and are an important world-wide food safety concern. Among the most relevant mycotoxigenic producer fungi are some Aspergillus species in particular those belonging to the Aspergillus section Flavi. These are known to produce the highly carcinogenic aflatoxins in agricultural commodities. Due to its impact in animal and human health, these species are among the most intenSively studied ones, being well known producers of aflatoxins (AFB1 , AFB2; AFG1 and AFG2) and cyciopiazonic acid (CPA). Aflatoxins are mainly produced by some strains of Aspergillus flavus and Aspergillus nomius and by most, if not all, strains of Aspergillus parasiticus. On the other hand, cyclopiazonic acid, which naturally occurs in a large variety of crop products as a co-contaminant with aflatoxins, is mainly produced by Aspergillus flavus strains. Together they have been shown to cause health problems in animals and humans, resulting in important economic losses. The production of CPA by Aspergillus section Flavi may also be routinely used for identification purposes since A. parasiticus, A. flavus and A. nomius, exhibit different mycotoxin profiles. The detection and quantification of both these mycotoxins is usually done separately by HPLC with UV detection for CPA and fluorescence detection after post-column derivatization for aflatoxins. There isn't a chromatographic method available to detect simultaneously CPA and the main four aflatoxins.porRapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section FlaviSoares, CéliaPersonalRodrigues, PaulaDSpacehttp://dspace.org/items/b864b8c1-d66a-419c-b1ac-e6193f93eca6DSpacehttp://dspace.org/items/b864b8c1-d66a-419c-b1ac-e6193f93eca6RodriguesPaulaCiência IDhttps://www.ciencia-id.pt2312-18AE-DA42ORCIDhttp://orcid.org0000-0002-3789-2730Scopus Author IDhttps://www.scopus.com7102639646Freitas-Silva, OtnielAbrunhosa, LuísVenâncio, ArmandoHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2011-05-12T09:40:48Z20102010-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/4240http://purl.org/coar/access_right/c_14cbmetadata only accessMultimycotoxin detectionFilamentous fungiFundação para a Ciência e a TecnologiaAVALIAÇÃO DA INCIDÊNCIA DE FUNGOS FILAMENTOSOS E DE MICOTOXINAS EM MILHO NACIONALCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaDESCONTAMINAÇAO BIOLÓGICA DE OCRATOXINA A UTILIZANDO UM ENZIMA DE A. NIGERCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCARACTERIZAÇÃO DA MICOFLORA E DO PERFIL DE MICOTOXINAS NA AMÊNDOA E CASTANHA DE TRÁS-OS-MONTES DESDE A PRODUÇÃO À COMERCIALIZAÇÃOCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objectProceedings of the VI Latinamerican Congress of Mycotoxicology and II International Symposium on Fungal and Algal Toxins in Industry.Merida, Mexico
spellingShingle Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
Soares, Célia
Multimycotoxin detection
Filamentous fungi
status SINGLETON
subject.fl_str_mv Multimycotoxin detection
Filamentous fungi
title Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
title_full Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
title_fullStr Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
title_full_unstemmed Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
title_short Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
title_sort Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic from Aspergillus section Flavi
topic Multimycotoxin detection
Filamentous fungi
topic_facet Multimycotoxin detection
Filamentous fungi
url http://hdl.handle.net/10198/4240
visible 1