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Chemical characterization and bioactivity evaluation of black mulberry and grape seeds

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Resumo:Circular economy is increasingly seen as a crucial way to reduce food by- products and residues and obtain valuable raw materials. There is a growing demand for industrial products with clean labels and bio benefits, where natural bioactive compounds play an important role. Among these natural biomolecules, polyphenols stand out for their health benefits against diseases such as cancer or diabetes. Thus, the use of agro-industrial bioresidues as a rich source of polyphenols for the development of new natural preservatives, colorants and nutraceuticals is in the spotlight [1,2]. The aim of this work was the recovery of polyphenols from grape and mulberry seeds, usually considered as biowaste. Polyphenols were characterized by HPLC-DAD- ESI/MSn, and the obtained polyphenol-rich extracts were analysed for their bioactive properties, namely antimicrobial and antioxidant capacities. Grape seeds were richer in catechin oligomers (36.0 ± 0.3 mg/g), and mulberry seeds in ellagic acid derivatives (3.14 ± 0.02 mg/g). In addition, both extracts exhibited high antimicrobial activity against multi-resistant Staphylococcus aureus. On the other hand, mulberry seeds showed the strongest antioxidant activity by the lipid peroxidation inhibition (IC50 = 23 ± 2 μg/mL) and oxidative haemolysis inhibition (IC50 at a 60 min Δt = 46.0 ± 0.8 μg/mL) methods [2]. Both by-products could be exploited for the recovery of added-value bioactive polyphenols and further application in the industry [2].
Autores principais:Gómez-Mejía, Esther
Outros Autores:Roriz, Custódio Lobo; Heleno, Sandrina A.; Calhelha, Ricardo C.; Dias, Maria Inês; Pinela, José; Rosales-Conrado, Noelia; León-González, María Eugenia; Ferreira, Isabel C.F.R.; Barros, Lillian
Assunto:Bioactivity Polyphenols By-products Grape/mulberry seeds
Ano:2021
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Gómez-Mejía, Esther
author2 Roriz, Custódio Lobo
Heleno, Sandrina A.
Calhelha, Ricardo C.
Dias, Maria Inês
Pinela, José
Rosales-Conrado, Noelia
León-González, María Eugenia
Ferreira, Isabel C.F.R.
Barros, Lillian
author2_role author
author
author
author
author
author
author
author
author
author_facet Gómez-Mejía, Esther
Roriz, Custódio Lobo
Heleno, Sandrina A.
Calhelha, Ricardo C.
Dias, Maria Inês
Pinela, José
Rosales-Conrado, Noelia
León-González, María Eugenia
Ferreira, Isabel C.F.R.
Barros, Lillian
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_str [{\"Person.name\":\"Gómez-Mejía, Esther\"},{\"Person.name\":\"Roriz, Custódio Lobo\",\"Person.identifier.orcid\":\"0000-0002-4021-1078\"},{\"Person.name\":\"Heleno, Sandrina A.\",\"Person.identifier.orcid\":\"0000-0001-7224-1098\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Dias, Maria Inês\",\"Person.identifier.orcid\":\"0000-0001-8744-7814\"},{\"Person.name\":\"Pinela, José\",\"Person.identifier.orcid\":\"0000-0001-7523-1637\"},{\"Person.name\":\"Rosales-Conrado, Noelia\"},{\"Person.name\":\"León-González, María Eugenia\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Gómez-Mejía, Esther
Roriz, Custódio Lobo
Heleno, Sandrina A.
Calhelha, Ricardo C.
Dias, Maria Inês
Pinela, José
Rosales-Conrado, Noelia
León-González, María Eugenia
Ferreira, Isabel C.F.R.
Barros, Lillian
datacite.date.Accepted.fl_str_mv 2021-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2022-01-25T11:57:04Z
datacite.date.embargoed.fl_str_mv 2022-01-25T11:57:04Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Bioactivity
Polyphenols
By-products
Grape/mulberry seeds
datacite.titles.title.fl_str_mv Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Gómez-Mejía, Esther
Roriz, Custódio Lobo
Heleno, Sandrina A.
Calhelha, Ricardo C.
Dias, Maria Inês
Pinela, José
Rosales-Conrado, Noelia
León-González, María Eugenia
Ferreira, Isabel C.F.R.
Barros, Lillian
dc.date.Accepted.fl_str_mv 2021-01-01T00:00:00Z
dc.date.available.fl_str_mv 2022-01-25T11:57:04Z
dc.date.embargoed.fl_str_mv 2022-01-25T11:57:04Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/24898
dc.language.none.fl_str_mv eng
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Bioactivity
Polyphenols
By-products
Grape/mulberry seeds
dc.title.fl_str_mv Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description Circular economy is increasingly seen as a crucial way to reduce food by- products and residues and obtain valuable raw materials. There is a growing demand for industrial products with clean labels and bio benefits, where natural bioactive compounds play an important role. Among these natural biomolecules, polyphenols stand out for their health benefits against diseases such as cancer or diabetes. Thus, the use of agro-industrial bioresidues as a rich source of polyphenols for the development of new natural preservatives, colorants and nutraceuticals is in the spotlight [1,2]. The aim of this work was the recovery of polyphenols from grape and mulberry seeds, usually considered as biowaste. Polyphenols were characterized by HPLC-DAD- ESI/MSn, and the obtained polyphenol-rich extracts were analysed for their bioactive properties, namely antimicrobial and antioxidant capacities. Grape seeds were richer in catechin oligomers (36.0 ± 0.3 mg/g), and mulberry seeds in ellagic acid derivatives (3.14 ± 0.02 mg/g). In addition, both extracts exhibited high antimicrobial activity against multi-resistant Staphylococcus aureus. On the other hand, mulberry seeds showed the strongest antioxidant activity by the lipid peroxidation inhibition (IC50 = 23 ± 2 μg/mL) and oxidative haemolysis inhibition (IC50 at a 60 min Δt = 46.0 ± 0.8 μg/mL) methods [2]. Both by-products could be exploited for the recovery of added-value bioactive polyphenols and further application in the industry [2].
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funding.funder.name_str_mv Fundação para a Ciência e a Tecnologia
Fundação para a Ciência e a Tecnologia
funding.name_str_mv 6817 - DCRRNI ID
POR_NORTE
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identifier.url.fl_str_mv http://hdl.handle.net/10198/24898
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person_str_mv Gómez-Mejía, Esther
Roriz, Custódio Lobo
Roriz, Custódio Lobo
https://www.ciencia-id.pt/9519-D97C-6B17
9519-D97C-6B17
http://orcid.org/0000-0002-4021-1078
0000-0002-4021-1078
Heleno, Sandrina A.
Heleno, Sandrina A.
https://www.ciencia-id.pt/8B18-3095-6FC9
8B18-3095-6FC9
http://orcid.org/0000-0001-7224-1098
0000-0001-7224-1098
Calhelha, Ricardo C.
Calhelha, Ricardo C.
https://www.ciencia-id.pt/F313-E3CE-554E
F313-E3CE-554E
http://orcid.org/0000-0002-6801-4578
0000-0002-6801-4578
Dias, Maria Inês
Dias, Maria Inês
https://www.ciencia-id.pt/2A13-4BE6-C7CF
2A13-4BE6-C7CF
http://orcid.org/0000-0001-8744-7814
0000-0001-8744-7814
Pinela, José
Pinela, José
https://www.ciencia-id.pt/771C-2B43-B108
771C-2B43-B108
http://orcid.org/0000-0001-7523-1637
0000-0001-7523-1637
Rosales-Conrado, Noelia
León-González, María Eugenia
Ferreira, Isabel C.F.R.
Ferreira, Isabel C.F.R.
https://www.ciencia-id.pt/9418-CF95-9919
9418-CF95-9919
http://orcid.org/0000-0003-4910-4882
0000-0003-4910-4882
Barros, Lillian
Barros, Lillian
https://www.ciencia-id.pt/9616-35CB-D001
9616-35CB-D001
http://orcid.org/0000-0002-9050-5189
0000-0002-9050-5189
publishDate 2021
reponame_str Biblioteca Digital do IPB
repository_id_str urn:repositoryAcronym:ipb
service_str_mv urn:repositoryAcronym:ipb
spelling engpt_PTCircular economy is increasingly seen as a crucial way to reduce food by- products and residues and obtain valuable raw materials. There is a growing demand for industrial products with clean labels and bio benefits, where natural bioactive compounds play an important role. Among these natural biomolecules, polyphenols stand out for their health benefits against diseases such as cancer or diabetes. Thus, the use of agro-industrial bioresidues as a rich source of polyphenols for the development of new natural preservatives, colorants and nutraceuticals is in the spotlight [1,2]. The aim of this work was the recovery of polyphenols from grape and mulberry seeds, usually considered as biowaste. Polyphenols were characterized by HPLC-DAD- ESI/MSn, and the obtained polyphenol-rich extracts were analysed for their bioactive properties, namely antimicrobial and antioxidant capacities. Grape seeds were richer in catechin oligomers (36.0 ± 0.3 mg/g), and mulberry seeds in ellagic acid derivatives (3.14 ± 0.02 mg/g). In addition, both extracts exhibited high antimicrobial activity against multi-resistant Staphylococcus aureus. On the other hand, mulberry seeds showed the strongest antioxidant activity by the lipid peroxidation inhibition (IC50 = 23 ± 2 μg/mL) and oxidative haemolysis inhibition (IC50 at a 60 min Δt = 46.0 ± 0.8 μg/mL) methods [2]. Both by-products could be exploited for the recovery of added-value bioactive polyphenols and further application in the industry [2].application/pdfpt_PTChemical characterization and bioactivity evaluation of black mulberry and grape seedsGómez-Mejía, EstherPersonalRoriz, Custódio LoboDSpacehttp://dspace.org/items/d13ab9dd-af85-4652-bae4-88b2a9034d79DSpacehttp://dspace.org/items/d13ab9dd-af85-4652-bae4-88b2a9034d79RorizCustódio LoboCiência IDhttps://www.ciencia-id.pt9519-D97C-6B17ORCIDhttp://orcid.org0000-0002-4021-1078Scopus Author IDhttps://www.scopus.com56151637600PersonalHeleno, Sandrina A.DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676HelenoSandrina A.Ciência IDhttps://www.ciencia-id.pt8B18-3095-6FC9ORCIDhttp://orcid.org0000-0001-7224-1098Researcher IDhttps://www.researcherid.comL-5951-2014Scopus Author IDhttps://www.scopus.com30067731800PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalDias, Maria InêsDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDiasMaria InêsCiência IDhttps://www.ciencia-id.pt2A13-4BE6-C7CFORCIDhttp://orcid.org0000-0001-8744-7814Researcher IDhttps://www.researcherid.comM-8242-2013Scopus Author IDhttps://www.scopus.com54388787000PersonalPinela, JoséDSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4DSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4PinelaJoséCiência IDhttps://www.ciencia-id.pt771C-2B43-B108ORCIDhttp://orcid.org0000-0001-7523-1637Researcher IDhttps://www.researcherid.comB-4466-2014Scopus Author IDhttps://www.scopus.com54392272800Rosales-Conrado, NoeliaLeón-González, María EugeniaPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf978-83-65955-53-12022-01-25T11:57:04Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/24898http://purl.org/coar/access_right/c_abf2open accessBioactivityPolyphenolsBy-productsGrape/mulberry seeds748796 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaDevelopment of a new colouring agent from Gomphrena globosa L.: optimization of the extraction process, stability studies and proof-of-concept in a food matrixPOR_NORTECrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2021http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/65d86501-e078-4b7a-b2a7-55ed3eb4e553/download1st International Meeting for Young Analytical Chemists
spellingShingle Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
Gómez-Mejía, Esther
Bioactivity
Polyphenols
By-products
Grape/mulberry seeds
subject.fl_str_mv Bioactivity
Polyphenols
By-products
Grape/mulberry seeds
title Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
title_full Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
title_fullStr Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
title_full_unstemmed Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
title_short Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
title_sort Chemical characterization and bioactivity evaluation of black mulberry and grape seeds
topic Bioactivity
Polyphenols
By-products
Grape/mulberry seeds
topic_facet Bioactivity
Polyphenols
By-products
Grape/mulberry seeds
url http://hdl.handle.net/10198/24898
visible 1