Publicação
Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry
| Resumo: | Mushrooms are known all over the world both due to the remarkable gastronomic value of some species and for severe intoxications mediated by other species that are frequently difficult to distinguish from the edible ones, by the common user. Therefore, it is important to develop strategies to discover molecules that can identify mushroom species. In the present work, two GC–MS methodologies were applied in the chemical characterization of 22 mushroom species (12 edible, 3 toxic and 7 potentially toxic) – a multi-target procedure to simultaneously determine amino acids (AA), fatty acids (FA) and sterols by previous derivatization procedure with MSTFA, and a Head Space-Solid Phase Microextraction method to determine volatiles. For both methods, two approaches to data analysis were used: (I) targeted analysis, to identify and quantify AA, FA sterols and volatiles; (II) untargeted analysis, including Principal Component Analysis and Partial Least Square Discriminant Analysis, in order to identify metabolites/metabolite pattern with potential species identification and/or differentiation. Multi-target experiment allowed the identification and quantification of twenty one primary metabolites (9 AA, 11 FA and 1 sterol). Furthermore, through untargeted data analysis, it was possible to identify a 5- carbon sugar alcohol structure molecule, which was tentatively identified as xylitol or adonitol, with potential to be a species-marker of the edible Suillus bovinus mushrooms. Volatile profiling studies resulted in the identification of the main volatiles in mushrooms. Untargeted analysis allowed the identification of 6 molecules that can be species- or genus-specific: one secondary metabolite specific to the edible species Lycoperdon perlatum, an ester of hexanoic acid, tentatively identified as allyl or vinyl caproate; and five other secondary metabolites, whose identification was not achieved, which were only detected in Lactarius aurantiacus specimens (edibility/toxicity unknown). |
|---|---|
| Autores principais: | Carvalho, Luís Miguel |
| Outros Autores: | Carvalho, Félix; Bastos, Maria de Lourdes; Baptista, Paula; Moreira, Nathalie; Monforte, Ana Rita; Ferreira, António César da Silva; Pinho, P. Guedes de |
| Assunto: | Edible and toxic wild mushrooms HS-SPME GC-IT/MS Volatiles Species discrimination Targeted and non-targeted analysis Principal component analysis (PCA) Partial least square discriminant Analysis (PLS-DA) |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851247324364800 |
|---|---|
| author | Carvalho, Luís Miguel |
| author2 | Carvalho, Félix Bastos, Maria de Lourdes Baptista, Paula Moreira, Nathalie Monforte, Ana Rita Ferreira, António César da Silva Pinho, P. Guedes de |
| author2_role | author author author author author author author |
| author_facet | Carvalho, Luís Miguel Carvalho, Félix Bastos, Maria de Lourdes Baptista, Paula Moreira, Nathalie Monforte, Ana Rita Ferreira, António César da Silva Pinho, P. Guedes de |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Carvalho, Luís Miguel\"},{\"Person.name\":\"Carvalho, Félix\"},{\"Person.name\":\"Bastos, Maria de Lourdes\"},{\"Person.name\":\"Baptista, Paula\",\"Person.identifier.orcid\":\"0000-0001-6331-3731\"},{\"Person.name\":\"Moreira, Nathalie\"},{\"Person.name\":\"Monforte, Ana Rita\"},{\"Person.name\":\"Ferreira, António César da Silva\"},{\"Person.name\":\"Pinho, P. Guedes de\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Carvalho, Luís Miguel Carvalho, Félix Bastos, Maria de Lourdes Baptista, Paula Moreira, Nathalie Monforte, Ana Rita Ferreira, António César da Silva Pinho, P. Guedes de |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-09-19T16:27:03Z |
| datacite.date.embargoed.fl_str_mv | 2014-09-19T16:27:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Edible and toxic wild mushrooms HS-SPME GC-IT/MS Volatiles Species discrimination Targeted and non-targeted analysis Principal component analysis (PCA) Partial least square discriminant Analysis (PLS-DA) |
| datacite.titles.title.fl_str_mv | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Carvalho, Luís Miguel Carvalho, Félix Bastos, Maria de Lourdes Baptista, Paula Moreira, Nathalie Monforte, Ana Rita Ferreira, António César da Silva Pinho, P. Guedes de |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-09-19T16:27:03Z |
| dc.date.embargoed.fl_str_mv | 2014-09-19T16:27:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/10508 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Edible and toxic wild mushrooms HS-SPME GC-IT/MS Volatiles Species discrimination Targeted and non-targeted analysis Principal component analysis (PCA) Partial least square discriminant Analysis (PLS-DA) |
| dc.title.fl_str_mv | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Mushrooms are known all over the world both due to the remarkable gastronomic value of some species and for severe intoxications mediated by other species that are frequently difficult to distinguish from the edible ones, by the common user. Therefore, it is important to develop strategies to discover molecules that can identify mushroom species. In the present work, two GC–MS methodologies were applied in the chemical characterization of 22 mushroom species (12 edible, 3 toxic and 7 potentially toxic) – a multi-target procedure to simultaneously determine amino acids (AA), fatty acids (FA) and sterols by previous derivatization procedure with MSTFA, and a Head Space-Solid Phase Microextraction method to determine volatiles. For both methods, two approaches to data analysis were used: (I) targeted analysis, to identify and quantify AA, FA sterols and volatiles; (II) untargeted analysis, including Principal Component Analysis and Partial Least Square Discriminant Analysis, in order to identify metabolites/metabolite pattern with potential species identification and/or differentiation. Multi-target experiment allowed the identification and quantification of twenty one primary metabolites (9 AA, 11 FA and 1 sterol). Furthermore, through untargeted data analysis, it was possible to identify a 5- carbon sugar alcohol structure molecule, which was tentatively identified as xylitol or adonitol, with potential to be a species-marker of the edible Suillus bovinus mushrooms. Volatile profiling studies resulted in the identification of the main volatiles in mushrooms. Untargeted analysis allowed the identification of 6 molecules that can be species- or genus-specific: one secondary metabolite specific to the edible species Lycoperdon perlatum, an ester of hexanoic acid, tentatively identified as allyl or vinyl caproate; and five other secondary metabolites, whose identification was not achieved, which were only detected in Lactarius aurantiacus specimens (edibility/toxicity unknown). |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/e8893633-0c98-4dc2-9963-068e37f1c655/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6820 - DCRRNI ID FARH |
| id | ipb_33d2d4ea06d2700e499a315d9953bd2a |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/10508 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/10508 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Carvalho, Luís Miguel Carvalho, Félix Bastos, Maria de Lourdes Baptista, Paula Baptista, Paula https://www.ciencia-id.pt/7D11-FE1E-CD0F 7D11-FE1E-CD0F http://orcid.org/0000-0001-6331-3731 0000-0001-6331-3731 Moreira, Nathalie Monforte, Ana Rita Ferreira, António César da Silva Pinho, P. Guedes de |
| publishDate | 2014 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engElsevierporMushrooms are known all over the world both due to the remarkable gastronomic value of some species and for severe intoxications mediated by other species that are frequently difficult to distinguish from the edible ones, by the common user. Therefore, it is important to develop strategies to discover molecules that can identify mushroom species. In the present work, two GC–MS methodologies were applied in the chemical characterization of 22 mushroom species (12 edible, 3 toxic and 7 potentially toxic) – a multi-target procedure to simultaneously determine amino acids (AA), fatty acids (FA) and sterols by previous derivatization procedure with MSTFA, and a Head Space-Solid Phase Microextraction method to determine volatiles. For both methods, two approaches to data analysis were used: (I) targeted analysis, to identify and quantify AA, FA sterols and volatiles; (II) untargeted analysis, including Principal Component Analysis and Partial Least Square Discriminant Analysis, in order to identify metabolites/metabolite pattern with potential species identification and/or differentiation. Multi-target experiment allowed the identification and quantification of twenty one primary metabolites (9 AA, 11 FA and 1 sterol). Furthermore, through untargeted data analysis, it was possible to identify a 5- carbon sugar alcohol structure molecule, which was tentatively identified as xylitol or adonitol, with potential to be a species-marker of the edible Suillus bovinus mushrooms. Volatile profiling studies resulted in the identification of the main volatiles in mushrooms. Untargeted analysis allowed the identification of 6 molecules that can be species- or genus-specific: one secondary metabolite specific to the edible species Lycoperdon perlatum, an ester of hexanoic acid, tentatively identified as allyl or vinyl caproate; and five other secondary metabolites, whose identification was not achieved, which were only detected in Lactarius aurantiacus specimens (edibility/toxicity unknown).application/pdfporNon-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometryCarvalho, Luís MiguelCarvalho, FélixBastos, Maria de LourdesPersonalBaptista, PaulaDSpacehttp://dspace.org/items/3f35226a-b17a-4f7d-8da1-3297105cbfe9DSpacehttp://dspace.org/items/3f35226a-b17a-4f7d-8da1-3297105cbfe9BaptistaPaulaCiência IDhttps://www.ciencia-id.pt7D11-FE1E-CD0FORCIDhttp://orcid.org0000-0001-6331-3731Scopus Author IDhttps://www.scopus.com14051688000Scopus Author IDhttps://www.scopus.com57394839600Moreira, NathalieMonforte, Ana RitaFerreira, António César da SilvaPinho, P. Guedes deHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0039-9140DOIIsPartOf10.1016/j.talanta.2013.09.0382014-09-19T16:27:03Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/10508http://purl.org/coar/access_right/c_16ecrestricted accessEdible and toxic wild mushroomsHS-SPME GC-IT/MSVolatilesSpecies discriminationTargeted and non-targeted analysisPrincipal component analysis (PCA)Partial least square discriminantAnalysis (PLS-DA)1707798 bytesFundação para a Ciência e a TecnologiaStrategic Project - LA 6 - 2011-20126820 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaSULPHUR COMPOUNDS IN GRAPES AND WINES FROM PORTUGUESE VARIETIES: CHEMICAL AND ORGANOLEPTIC PROFILE CHARACTERIZATION; BIOAVAILABILITY AND BIOLOGICAL ACTIVITY STUDIESFARHCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/e8893633-0c98-4dc2-9963-068e37f1c655/downloadTalanta118292303 |
| spellingShingle | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry Carvalho, Luís Miguel Edible and toxic wild mushrooms HS-SPME GC-IT/MS Volatiles Species discrimination Targeted and non-targeted analysis Principal component analysis (PCA) Partial least square discriminant Analysis (PLS-DA) |
| subject.fl_str_mv | Edible and toxic wild mushrooms HS-SPME GC-IT/MS Volatiles Species discrimination Targeted and non-targeted analysis Principal component analysis (PCA) Partial least square discriminant Analysis (PLS-DA) |
| title | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| title_full | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| title_fullStr | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| title_full_unstemmed | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| title_short | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| title_sort | Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry |
| topic | Edible and toxic wild mushrooms HS-SPME GC-IT/MS Volatiles Species discrimination Targeted and non-targeted analysis Principal component analysis (PCA) Partial least square discriminant Analysis (PLS-DA) |
| topic_facet | Edible and toxic wild mushrooms HS-SPME GC-IT/MS Volatiles Species discrimination Targeted and non-targeted analysis Principal component analysis (PCA) Partial least square discriminant Analysis (PLS-DA) |
| url | http://hdl.handle.net/10198/10508 |
| visible | 1 |