Publicação
Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay
| Resumo: | Polymerase chain reaction (PCR)-ampliÞed mitochondrialDNA(mtDNA)assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all known introduced subspecies in the United States, and 451 colonies from non-Africanized areas of the southern United States were screened to validate a rapid PCR-based assay for identiÞcation of Africanized honey bee mtDNA. This PCR-based assay requires a single enzyme digestion (BglII) of a single PCR-ampliÞed segment of the cytochrome b gene. The BglII polymorphism discriminates the mitochondrial haplotype (mitotype) of Apis mellifera scutellata L. (ancestor of Africanized bees) from that of A. m. mellifera, A. m. caucasia, A. m. ligustica, A. m. carnica, A. m. lamarcki, A. m. cypria, A. m. syriaca, and some A. m. iberiensis, but not from that of A. m. intermissa and some A. m. iberiensis. Nonetheless, given the very low frequency ( 1%) of African non-A. m. scutellata mitotype present before arrival of Africanized bees in the United States, cytochrome b/BglII assay can be used to identify maternally Africanized bees with a high degree of reliability. |
|---|---|
| Autores principais: | Pinto, M. Alice |
| Outros Autores: | Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Patton, John C.; Sheppard, Walter S. |
| Assunto: | Africanized honeybee A. m. scutellata Mitochondrial DNA mtDNA haplotype Mitotype |
| Ano: | 2003 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | português |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172730635288576 |
|---|---|
| author | Pinto, M. Alice |
| author2 | Johnston, J. Spencer Rubink, William L. Coulson, Robert N. Patton, John C. Sheppard, Walter S. |
| author2_role | author author author author author |
| author_facet | Pinto, M. Alice Johnston, J. Spencer Rubink, William L. Coulson, Robert N. Patton, John C. Sheppard, Walter S. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"},{\"Person.name\":\"Johnston, J. Spencer\"},{\"Person.name\":\"Rubink, William L.\"},{\"Person.name\":\"Coulson, Robert N.\"},{\"Person.name\":\"Patton, John C.\"},{\"Person.name\":\"Sheppard, Walter S.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pinto, M. Alice Johnston, J. Spencer Rubink, William L. Coulson, Robert N. Patton, John C. Sheppard, Walter S. |
| datacite.date.Accepted.fl_str_mv | 2003-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2010-12-07T15:28:45Z |
| datacite.date.embargoed.fl_str_mv | 2010-12-07T15:28:45Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Africanized honeybee A. m. scutellata Mitochondrial DNA mtDNA haplotype Mitotype |
| datacite.titles.title.fl_str_mv | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pinto, M. Alice Johnston, J. Spencer Rubink, William L. Coulson, Robert N. Patton, John C. Sheppard, Walter S. |
| dc.date.Accepted.fl_str_mv | 2003-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2010-12-07T15:28:45Z |
| dc.date.embargoed.fl_str_mv | 2010-12-07T15:28:45Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/2902 |
| dc.language.none.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | Entomological Society of America |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Africanized honeybee A. m. scutellata Mitochondrial DNA mtDNA haplotype Mitotype |
| dc.title.fl_str_mv | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Polymerase chain reaction (PCR)-ampliÞed mitochondrialDNA(mtDNA)assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all known introduced subspecies in the United States, and 451 colonies from non-Africanized areas of the southern United States were screened to validate a rapid PCR-based assay for identiÞcation of Africanized honey bee mtDNA. This PCR-based assay requires a single enzyme digestion (BglII) of a single PCR-ampliÞed segment of the cytochrome b gene. The BglII polymorphism discriminates the mitochondrial haplotype (mitotype) of Apis mellifera scutellata L. (ancestor of Africanized bees) from that of A. m. mellifera, A. m. caucasia, A. m. ligustica, A. m. carnica, A. m. lamarcki, A. m. cypria, A. m. syriaca, and some A. m. iberiensis, but not from that of A. m. intermissa and some A. m. iberiensis. Nonetheless, given the very low frequency ( 1%) of African non-A. m. scutellata mitotype present before arrival of Africanized bees in the United States, cytochrome b/BglII assay can be used to identify maternally Africanized bees with a high degree of reliability. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/54622be5-4e3d-44a6-8c7b-648fa981af74/download |
| id | ipb_35d471dc2fa5f325e2377d52f4b720dd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/2902 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | por |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/2902 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 Johnston, J. Spencer Rubink, William L. Coulson, Robert N. Patton, John C. Sheppard, Walter S. |
| publishDate | 2003 |
| publisher.none.fl_str_mv | Entomological Society of America |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | porEntomological Society of AmericaporPolymerase chain reaction (PCR)-ampliÞed mitochondrialDNA(mtDNA)assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all known introduced subspecies in the United States, and 451 colonies from non-Africanized areas of the southern United States were screened to validate a rapid PCR-based assay for identiÞcation of Africanized honey bee mtDNA. This PCR-based assay requires a single enzyme digestion (BglII) of a single PCR-ampliÞed segment of the cytochrome b gene. The BglII polymorphism discriminates the mitochondrial haplotype (mitotype) of Apis mellifera scutellata L. (ancestor of Africanized bees) from that of A. m. mellifera, A. m. caucasia, A. m. ligustica, A. m. carnica, A. m. lamarcki, A. m. cypria, A. m. syriaca, and some A. m. iberiensis, but not from that of A. m. intermissa and some A. m. iberiensis. Nonetheless, given the very low frequency ( 1%) of African non-A. m. scutellata mitotype present before arrival of Africanized bees in the United States, cytochrome b/BglII assay can be used to identify maternally Africanized bees with a high degree of reliability.application/pdfporIdentification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assayPersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800Johnston, J. SpencerRubink, William L.Coulson, Robert N.Patton, John C.Sheppard, Walter S.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0013-8746DOIIsPartOf10.1603/0013-8746(2003)096[0679:IOAHBH]2.0.CO;22010-12-07T15:28:45Z20032003-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/2902http://purl.org/coar/access_right/c_abf2open accessAfricanized honeybeeA. m. scutellataMitochondrial DNAmtDNA haplotypeMitotype364401 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/54622be5-4e3d-44a6-8c7b-648fa981af74/downloadAnnals of the Entomological Society of America679684 |
| spellingShingle | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay Pinto, M. Alice Africanized honeybee A. m. scutellata Mitochondrial DNA mtDNA haplotype Mitotype |
| status | SINGLETON |
| subject.fl_str_mv | Africanized honeybee A. m. scutellata Mitochondrial DNA mtDNA haplotype Mitotype |
| title | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| title_full | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| title_fullStr | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| title_full_unstemmed | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| title_short | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| title_sort | Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay |
| topic | Africanized honeybee A. m. scutellata Mitochondrial DNA mtDNA haplotype Mitotype |
| topic_facet | Africanized honeybee A. m. scutellata Mitochondrial DNA mtDNA haplotype Mitotype |
| url | http://hdl.handle.net/10198/2902 |
| visible | 1 |