Publicação
Malpighia emarginata DC. as a source of nutrients and bioactive compounds
| Resumo: | From the Industrial Revolution, humanity has been suffering the consequences of a system that only focus on economic growth, neglecting environment quality and, consequently, population’s health. Horticulture is one of the industrial sectors where the greatest amount of organic waste is generated.1 These biowastes are further discarded and devalued. However, these by-products are excellent sources of pigments, phenolic compounds, dietary fibres, sugar derivatives, organic acids, minerals and vitamins. All these compounds have health beneficial properties, so their reuse is an effective and promising alternative for industry and environment.2 Many authors have studied different vegetable matrices and found molecules of high industrial interest in their composition.3 Due to its high vitamin C, carotenoids and anthocyanins content, Malpighia emarginata DC. (acerola) is known for its nutritional and functional properties. Its extracts are used as substitutes for phytoestrogen in medicines and show biofunctional effects, such as antioxidant activity in the prevention of age-related diseases, cancer, cardiovascular problems and hypertension.4 Considering that, this research work would make a nutritional and chemical characterization of acerola bioresidues, as well as a study of the bioactive potential of its extract. The nutritional profile (protein, ash, fat, carbohydrate and energy content) was determined using official methodologies for the analysis of food products (AOAC). The chemical profile was evaluated, determining free sugars by HPLC-RI, organic acids by UFLC-PDA, fatty acids by GC-FID, tocopherols by HPLC-fluorescence and phenolic compounds (anthocyanin and non-anthocyanin) by HPLC-DAD-ESI/MS. The bioactive potential was assessed through in vitro tests for antioxidant, anti-inflammatory and antimicrobial activity, as well as cytotoxicity and hepatotoxicity tests on human tumour cell lines and non-tumour primary cell culture, respectively, by applying the sulforhodamine B assay. Fructose, glucose and sucrose were identified, highlighting fructose as the main free sugar compound. Also, oxalic, malic, ascorbic and succinic acids were identified, being malic acid the most abundant organic acid. The fatty acids profile revealed the presence of fourteen compounds, being mostly represented by saturated fatty acids, with oleic acid as the majority. The phenolic composition evidenced the presence of six non-anthocyanin compounds and two anthocyanins, standing out isorhamnetin-O-pentosyl- hexoside and cyanidin-O-deoxyhexoside as the major compounds, respectively. Regarding the bioactive action, the presence of antioxidant and anti-inflammatory action was demonstrated, as well as, the antimicrobial activity revealed promising results, with good bacteriostatic and fungistatic effects. Concerning cytotoxic evaluation, the extract manifested anti-proliferative capacity against the half-tested tumour cell lines and exhibit non-toxicity in the non-tumour cell cultures. Overall, this study highlights the excellent composition in compounds of interest and bioactive potential of Malpighia emarginata DC. fruits, being a promising option for the development of functional products in the food and pharmaceutical industry. |
|---|---|
| Autores principais: | Cerino, María Carolina |
| Outros Autores: | Caleja, Cristina; Calhelha, Ricardo C.; Dias, Maria Inês; Pereira, Carla; Stojković, Dejan; Soković, Marina; Ferreira, Isabel C.F.R.; Saux, Clara; Pereira, Eliana; Barros, Lillian |
| Assunto: | Malpighia emarginata DC. |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851406286389248 |
|---|---|
| author | Cerino, María Carolina |
| author2 | Caleja, Cristina Calhelha, Ricardo C. Dias, Maria Inês Pereira, Carla Stojković, Dejan Soković, Marina Ferreira, Isabel C.F.R. Saux, Clara Pereira, Eliana Barros, Lillian |
| author2_role | author author author author author author author author author author |
| author_facet | Cerino, María Carolina Caleja, Cristina Calhelha, Ricardo C. Dias, Maria Inês Pereira, Carla Stojković, Dejan Soković, Marina Ferreira, Isabel C.F.R. Saux, Clara Pereira, Eliana Barros, Lillian |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Cerino, María Carolina\"},{\"Person.name\":\"Caleja, Cristina\",\"Person.identifier.orcid\":\"0000-0002-5769-8484\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Dias, Maria Inês\",\"Person.identifier.orcid\":\"0000-0001-8744-7814\"},{\"Person.name\":\"Pereira, Carla\",\"Person.identifier.orcid\":\"0000-0003-0093-771X\"},{\"Person.name\":\"Stojković, Dejan\"},{\"Person.name\":\"Soković, Marina\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Saux, Clara\"},{\"Person.name\":\"Pereira, Eliana\",\"Person.identifier.orcid\":\"0000-0003-2854-6745\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Cerino, María Carolina Caleja, Cristina Calhelha, Ricardo C. Dias, Maria Inês Pereira, Carla Stojković, Dejan Soković, Marina Ferreira, Isabel C.F.R. Saux, Clara Pereira, Eliana Barros, Lillian |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-01-25T12:01:51Z |
| datacite.date.embargoed.fl_str_mv | 2022-01-25T12:01:51Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Malpighia emarginata DC. |
| datacite.titles.title.fl_str_mv | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Cerino, María Carolina Caleja, Cristina Calhelha, Ricardo C. Dias, Maria Inês Pereira, Carla Stojković, Dejan Soković, Marina Ferreira, Isabel C.F.R. Saux, Clara Pereira, Eliana Barros, Lillian |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-01-25T12:01:51Z |
| dc.date.embargoed.fl_str_mv | 2022-01-25T12:01:51Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/24899 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Malpighia emarginata DC. |
| dc.title.fl_str_mv | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | From the Industrial Revolution, humanity has been suffering the consequences of a system that only focus on economic growth, neglecting environment quality and, consequently, population’s health. Horticulture is one of the industrial sectors where the greatest amount of organic waste is generated.1 These biowastes are further discarded and devalued. However, these by-products are excellent sources of pigments, phenolic compounds, dietary fibres, sugar derivatives, organic acids, minerals and vitamins. All these compounds have health beneficial properties, so their reuse is an effective and promising alternative for industry and environment.2 Many authors have studied different vegetable matrices and found molecules of high industrial interest in their composition.3 Due to its high vitamin C, carotenoids and anthocyanins content, Malpighia emarginata DC. (acerola) is known for its nutritional and functional properties. Its extracts are used as substitutes for phytoestrogen in medicines and show biofunctional effects, such as antioxidant activity in the prevention of age-related diseases, cancer, cardiovascular problems and hypertension.4 Considering that, this research work would make a nutritional and chemical characterization of acerola bioresidues, as well as a study of the bioactive potential of its extract. The nutritional profile (protein, ash, fat, carbohydrate and energy content) was determined using official methodologies for the analysis of food products (AOAC). The chemical profile was evaluated, determining free sugars by HPLC-RI, organic acids by UFLC-PDA, fatty acids by GC-FID, tocopherols by HPLC-fluorescence and phenolic compounds (anthocyanin and non-anthocyanin) by HPLC-DAD-ESI/MS. The bioactive potential was assessed through in vitro tests for antioxidant, anti-inflammatory and antimicrobial activity, as well as cytotoxicity and hepatotoxicity tests on human tumour cell lines and non-tumour primary cell culture, respectively, by applying the sulforhodamine B assay. Fructose, glucose and sucrose were identified, highlighting fructose as the main free sugar compound. Also, oxalic, malic, ascorbic and succinic acids were identified, being malic acid the most abundant organic acid. The fatty acids profile revealed the presence of fourteen compounds, being mostly represented by saturated fatty acids, with oleic acid as the majority. The phenolic composition evidenced the presence of six non-anthocyanin compounds and two anthocyanins, standing out isorhamnetin-O-pentosyl- hexoside and cyanidin-O-deoxyhexoside as the major compounds, respectively. Regarding the bioactive action, the presence of antioxidant and anti-inflammatory action was demonstrated, as well as, the antimicrobial activity revealed promising results, with good bacteriostatic and fungistatic effects. Concerning cytotoxic evaluation, the extract manifested anti-proliferative capacity against the half-tested tumour cell lines and exhibit non-toxicity in the non-tumour cell cultures. Overall, this study highlights the excellent composition in compounds of interest and bioactive potential of Malpighia emarginata DC. fruits, being a promising option for the development of functional products in the food and pharmaceutical industry. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
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| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/24899 |
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| institution | Instituto Politécnico de Bragança |
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| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Cerino, María Carolina Caleja, Cristina Caleja, Cristina https://www.ciencia-id.pt/E218-8DD3-4959 E218-8DD3-4959 http://orcid.org/0000-0002-5769-8484 0000-0002-5769-8484 Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Dias, Maria Inês Dias, Maria Inês https://www.ciencia-id.pt/2A13-4BE6-C7CF 2A13-4BE6-C7CF http://orcid.org/0000-0001-8744-7814 0000-0001-8744-7814 Pereira, Carla Pereira, Carla https://www.ciencia-id.pt/EF10-2739-2B70 EF10-2739-2B70 http://orcid.org/0000-0003-0093-771X 0000-0003-0093-771X Stojković, Dejan Soković, Marina Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Saux, Clara Pereira, Eliana Pereira, Eliana https://www.ciencia-id.pt/BE17-462D-9A75 BE17-462D-9A75 http://orcid.org/0000-0003-2854-6745 0000-0003-2854-6745 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 |
| publishDate | 2021 |
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| spelling | engpt_PTFrom the Industrial Revolution, humanity has been suffering the consequences of a system that only focus on economic growth, neglecting environment quality and, consequently, population’s health. Horticulture is one of the industrial sectors where the greatest amount of organic waste is generated.1 These biowastes are further discarded and devalued. However, these by-products are excellent sources of pigments, phenolic compounds, dietary fibres, sugar derivatives, organic acids, minerals and vitamins. All these compounds have health beneficial properties, so their reuse is an effective and promising alternative for industry and environment.2 Many authors have studied different vegetable matrices and found molecules of high industrial interest in their composition.3 Due to its high vitamin C, carotenoids and anthocyanins content, Malpighia emarginata DC. (acerola) is known for its nutritional and functional properties. Its extracts are used as substitutes for phytoestrogen in medicines and show biofunctional effects, such as antioxidant activity in the prevention of age-related diseases, cancer, cardiovascular problems and hypertension.4 Considering that, this research work would make a nutritional and chemical characterization of acerola bioresidues, as well as a study of the bioactive potential of its extract. The nutritional profile (protein, ash, fat, carbohydrate and energy content) was determined using official methodologies for the analysis of food products (AOAC). The chemical profile was evaluated, determining free sugars by HPLC-RI, organic acids by UFLC-PDA, fatty acids by GC-FID, tocopherols by HPLC-fluorescence and phenolic compounds (anthocyanin and non-anthocyanin) by HPLC-DAD-ESI/MS. The bioactive potential was assessed through in vitro tests for antioxidant, anti-inflammatory and antimicrobial activity, as well as cytotoxicity and hepatotoxicity tests on human tumour cell lines and non-tumour primary cell culture, respectively, by applying the sulforhodamine B assay. Fructose, glucose and sucrose were identified, highlighting fructose as the main free sugar compound. Also, oxalic, malic, ascorbic and succinic acids were identified, being malic acid the most abundant organic acid. The fatty acids profile revealed the presence of fourteen compounds, being mostly represented by saturated fatty acids, with oleic acid as the majority. The phenolic composition evidenced the presence of six non-anthocyanin compounds and two anthocyanins, standing out isorhamnetin-O-pentosyl- hexoside and cyanidin-O-deoxyhexoside as the major compounds, respectively. Regarding the bioactive action, the presence of antioxidant and anti-inflammatory action was demonstrated, as well as, the antimicrobial activity revealed promising results, with good bacteriostatic and fungistatic effects. Concerning cytotoxic evaluation, the extract manifested anti-proliferative capacity against the half-tested tumour cell lines and exhibit non-toxicity in the non-tumour cell cultures. Overall, this study highlights the excellent composition in compounds of interest and bioactive potential of Malpighia emarginata DC. fruits, being a promising option for the development of functional products in the food and pharmaceutical industry.application/pdfpt_PTMalpighia emarginata DC. as a source of nutrients and bioactive compoundsCerino, María CarolinaPersonalCaleja, CristinaDSpacehttp://dspace.org/items/5ef2706d-073c-4c23-9e45-498ac244118cDSpacehttp://dspace.org/items/5ef2706d-073c-4c23-9e45-498ac244118cCalejaCristinaCiência IDhttps://www.ciencia-id.ptE218-8DD3-4959ORCIDhttp://orcid.org0000-0002-5769-8484Scopus Author IDhttps://www.scopus.com36514202500PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalDias, Maria InêsDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDiasMaria InêsCiência IDhttps://www.ciencia-id.pt2A13-4BE6-C7CFORCIDhttp://orcid.org0000-0001-8744-7814Researcher IDhttps://www.researcherid.comM-8242-2013Scopus Author IDhttps://www.scopus.com54388787000PersonalPereira, CarlaDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bPereiraCarlaCiência IDhttps://www.ciencia-id.ptEF10-2739-2B70ORCIDhttp://orcid.org0000-0003-0093-771XResearcher IDhttps://www.researcherid.comK-1629-2016Scopus Author IDhttps://www.scopus.com55627876424Stojković, DejanSoković, MarinaPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600Saux, ClaraPersonalPereira, ElianaDSpacehttp://dspace.org/items/dda60cdc-0a2a-4c10-863e-eaef1e6f1fb9DSpacehttp://dspace.org/items/dda60cdc-0a2a-4c10-863e-eaef1e6f1fb9PereiraElianaCiência IDhttps://www.ciencia-id.ptBE17-462D-9A75ORCIDhttp://orcid.org0000-0003-2854-6745Researcher IDhttps://www.researcherid.comQ-8017-2018Scopus Author IDhttps://www.scopus.com55938912300PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-989-8805-68-32022-01-25T12:01:51Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/24899http://purl.org/coar/access_right/c_abf2open accessMalpighia emarginata DC.622393 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2021http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/1e0d301e-2873-4dc9-8167-3874236d8977/downloadXV Encontro de Química dos AlimentosMadeira, Portugal |
| spellingShingle | Malpighia emarginata DC. as a source of nutrients and bioactive compounds Cerino, María Carolina Malpighia emarginata DC. |
| subject.fl_str_mv | Malpighia emarginata DC. |
| title | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| title_full | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| title_fullStr | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| title_full_unstemmed | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| title_short | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| title_sort | Malpighia emarginata DC. as a source of nutrients and bioactive compounds |
| topic | Malpighia emarginata DC. |
| topic_facet | Malpighia emarginata DC. |
| url | http://hdl.handle.net/10198/24899 |
| visible | 1 |