Publicação
Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers
| Resumo: | Photodynamic therapy (PDT) has been considered a promising treatment for several types of cancer, including cervical cancer. Localized drug delivery systems (DDSs) based on nanofibers produced by electrospinning have emerged as a powerful platform to carry and deliver photosensitizers (PSs) onto or adjacent to the tumor site, thereby promoting higher therapeutic efficacy and reducing the side effects to healthy tissues associated with systemic administration. In this work, core–shell electrospun nanofibers were produced using biodegradable polymers, such as poly(vinyl alcohol) (PVA) and gelatin (Gel), to act as a localized DDS for the treatment of cervical cancer using PDT. The synthesized porphyrin (Por) was able to generate singlet oxygen (FD = 0.62) and displayed higher phototoxicity against tumor cells compared with healthy cells. The developed PVA–Gel membranes were fully characterized, revealing defect-free nanofibers with a core–shell structure. Different Por concentrations were added to the fibers’ core, and their presence and uniform distribution within the nanofibers were confirmed. The Por release profile from nanofibers showed an initial fast release stage, followed by continuous release for at least 9 days. The PVA–Gel + Por core–shell nanofibers exhibited a higher inhibition of cancer cell proliferation under light irradiation when compared to dark and a higher phototoxic effect against tumor cells compared with non-tumor cells. Overall, this study demonstrates the great potential of core–shell nanofibers to be used as localized DDSs of PSs for the treatment of cervical cancer. |
|---|---|
| Autores principais: | Costa, Sofia M. |
| Outros Autores: | Lourenço, Leandro M.O.; Calhelha, Ricardo C.; Calejo, Isabel; Barrias, Cristina C.; Fangueiro, Raul; Ferreira, Diana P. |
| Assunto: | Photodynamic therapy Cervical cancer |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173197690961920 |
|---|---|
| author | Costa, Sofia M. |
| author2 | Lourenço, Leandro M.O. Calhelha, Ricardo C. Calejo, Isabel Barrias, Cristina C. Fangueiro, Raul Ferreira, Diana P. |
| author2_role | author author author author author author |
| author_facet | Costa, Sofia M. Lourenço, Leandro M.O. Calhelha, Ricardo C. Calejo, Isabel Barrias, Cristina C. Fangueiro, Raul Ferreira, Diana P. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Costa, Sofia M.\"},{\"Person.name\":\"Lourenço, Leandro M.O.\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Calejo, Isabel\"},{\"Person.name\":\"Barrias, Cristina C.\"},{\"Person.name\":\"Fangueiro, Raul\"},{\"Person.name\":\"Ferreira, Diana P.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Costa, Sofia M. Lourenço, Leandro M.O. Calhelha, Ricardo C. Calejo, Isabel Barrias, Cristina C. Fangueiro, Raul Ferreira, Diana P. |
| datacite.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-09-30T08:49:02Z |
| datacite.date.embargoed.fl_str_mv | 2024-09-30T08:49:02Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Photodynamic therapy Cervical cancer |
| datacite.titles.title.fl_str_mv | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Costa, Sofia M. Lourenço, Leandro M.O. Calhelha, Ricardo C. Calejo, Isabel Barrias, Cristina C. Fangueiro, Raul Ferreira, Diana P. |
| dc.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-09-30T08:49:02Z |
| dc.date.embargoed.fl_str_mv | 2024-09-30T08:49:02Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/30292 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Royal Society of Chemistry |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Photodynamic therapy Cervical cancer |
| dc.title.fl_str_mv | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Photodynamic therapy (PDT) has been considered a promising treatment for several types of cancer, including cervical cancer. Localized drug delivery systems (DDSs) based on nanofibers produced by electrospinning have emerged as a powerful platform to carry and deliver photosensitizers (PSs) onto or adjacent to the tumor site, thereby promoting higher therapeutic efficacy and reducing the side effects to healthy tissues associated with systemic administration. In this work, core–shell electrospun nanofibers were produced using biodegradable polymers, such as poly(vinyl alcohol) (PVA) and gelatin (Gel), to act as a localized DDS for the treatment of cervical cancer using PDT. The synthesized porphyrin (Por) was able to generate singlet oxygen (FD = 0.62) and displayed higher phototoxicity against tumor cells compared with healthy cells. The developed PVA–Gel membranes were fully characterized, revealing defect-free nanofibers with a core–shell structure. Different Por concentrations were added to the fibers’ core, and their presence and uniform distribution within the nanofibers were confirmed. The Por release profile from nanofibers showed an initial fast release stage, followed by continuous release for at least 9 days. The PVA–Gel + Por core–shell nanofibers exhibited a higher inhibition of cancer cell proliferation under light irradiation when compared to dark and a higher phototoxic effect against tumor cells compared with non-tumor cells. Overall, this study demonstrates the great potential of core–shell nanofibers to be used as localized DDSs of PSs for the treatment of cervical cancer. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/fc4dae2f-0691-44d9-bfa3-9ba744070500/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT FCT FCT FCT FCT FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | UIDP/00264/2020 UIDB/00264/2020 UIDB/50006/2020 UIDP/50006/2020 PTDC/BTM-ORG/5154/2020 DRI/India/0447/2020 UIDB/00690/2020 UIDP/00690/2020 LA/P/0007/2020 CEECIND/02803/2017/CP1458/CT0003 SFRH/BD/147517/2019 |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID 3599-PPCDT 3599-PPCDT 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID CEEC IND 2017 POR_NORTE |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/30292 |
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| language | eng |
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| person_str_mv | Costa, Sofia M. Lourenço, Leandro M.O. Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Calejo, Isabel Barrias, Cristina C. Fangueiro, Raul Ferreira, Diana P. |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Royal Society of Chemistry |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engRoyal Society of Chemistrypt_PTPhotodynamic therapy (PDT) has been considered a promising treatment for several types of cancer, including cervical cancer. Localized drug delivery systems (DDSs) based on nanofibers produced by electrospinning have emerged as a powerful platform to carry and deliver photosensitizers (PSs) onto or adjacent to the tumor site, thereby promoting higher therapeutic efficacy and reducing the side effects to healthy tissues associated with systemic administration. In this work, core–shell electrospun nanofibers were produced using biodegradable polymers, such as poly(vinyl alcohol) (PVA) and gelatin (Gel), to act as a localized DDS for the treatment of cervical cancer using PDT. The synthesized porphyrin (Por) was able to generate singlet oxygen (FD = 0.62) and displayed higher phototoxicity against tumor cells compared with healthy cells. The developed PVA–Gel membranes were fully characterized, revealing defect-free nanofibers with a core–shell structure. Different Por concentrations were added to the fibers’ core, and their presence and uniform distribution within the nanofibers were confirmed. The Por release profile from nanofibers showed an initial fast release stage, followed by continuous release for at least 9 days. The PVA–Gel + Por core–shell nanofibers exhibited a higher inhibition of cancer cell proliferation under light irradiation when compared to dark and a higher phototoxic effect against tumor cells compared with non-tumor cells. Overall, this study demonstrates the great potential of core–shell nanofibers to be used as localized DDSs of PSs for the treatment of cervical cancer.application/pdfpt_PTLocalized cancer photodynamic therapy approach based on core–shell electrospun nanofibersCosta, Sofia M.Lourenço, Leandro M.O.PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333Calejo, IsabelBarrias, Cristina C.Fangueiro, RaulFerreira, Diana P.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2633-5409DOIIsPartOf10.1039/D4MA00647J2024-09-30T08:49:02Z20242024-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/30292http://purl.org/coar/access_right/c_abf2open accessPhotodynamic therapyCervical cancer1560758 bytesFundação para a Ciência e a TecnologiaCentre for Textile Science and Technologyinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00264%2F2020/PTUIDP/00264/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCentre for Textile Science and Technologyinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00264%2F2020/PTUIDB/00264/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PTUIDB/50006/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PTUIDP/50006/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaHarnessing endothelial plasticity for therapeutic vascularization of ischemic tissues: a bioengineering approachinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-ORG%2F5154%2F2020/PTPTDC/BTM-ORG/5154/20203599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaPersonal Protective equipment for active filtration of microorganisms based on electrospun nanofiber membranes<br><br>info:eu-repo/grantAgreement/FCT/3599-PPCDT/DRI%2FIndia%2F0447%2F2020/PTDRI/India/0447/20203599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Centerinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PTUIDB/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Centerinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PTUIDP/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociate Laboratory for Sustainability and Tecnology in Mountain Regionsinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PTLA/P/0007/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaSmart, sustainable and innovative materials for personal protection and healthcare based on Natural Fibres and Nanomaterialsinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F02803%2F2017%2FCP1458%2FCT0003/PTCEECIND/02803/2017/CP1458/CT0003CEEC IND 2017Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaLocal drug delivery systems based on biodegradable nanofibers for cancer photodynamic therapy PDTinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F147517%2F2019/PTSFRH/BD/147517/2019POR_NORTECrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2024http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/fc4dae2f-0691-44d9-bfa3-9ba744070500/downloadMaterials Advances51664896500 |
| spellingShingle | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers Costa, Sofia M. Photodynamic therapy Cervical cancer |
| status | SINGLETON |
| subject.fl_str_mv | Photodynamic therapy Cervical cancer |
| title | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| title_full | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| title_fullStr | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| title_full_unstemmed | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| title_short | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| title_sort | Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers |
| topic | Photodynamic therapy Cervical cancer |
| topic_facet | Photodynamic therapy Cervical cancer |
| url | http://hdl.handle.net/10198/30292 |
| visible | 1 |
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