Publicação
Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products
| Resumo: | In this work, a polymerase chain reaction (PCR) technique was used for soybean detection in processed meat products. The methodology was optimized using reference binary samples with different known percentages of pork meat and soybean protein, prepared in the laboratory. To evaluate the effect of thermal treatment, binary mixtures were submitted to thermal treatment at 121ºC for 5 min. The species-specific PCR targeting the lectin gene allowed the detection limit of 0.01 ng of soybean DNA in raw materials. The PCR method was successfully applied on 18 commercial Frankfurt sausages. |
|---|---|
| Autores principais: | Amaral, Joana S. |
| Outros Autores: | Soares, Sónia; Mafra, Isabel; Oliveira, Beatriz |
| Assunto: | Soybean Allergens Meat products PCR |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | português |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173148883943424 |
|---|---|
| author | Amaral, Joana S. |
| author2 | Soares, Sónia Mafra, Isabel Oliveira, Beatriz |
| author2_role | author author author |
| author_facet | Amaral, Joana S. Soares, Sónia Mafra, Isabel Oliveira, Beatriz |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Amaral, Joana S.\",\"Person.identifier.orcid\":\"0000-0002-3648-7303\"},{\"Person.name\":\"Soares, Sónia\"},{\"Person.name\":\"Mafra, Isabel\"},{\"Person.name\":\"Oliveira, Beatriz\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Amaral, Joana S. Soares, Sónia Mafra, Isabel Oliveira, Beatriz |
| datacite.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-06-01T13:24:09Z |
| datacite.date.embargoed.fl_str_mv | 2011-06-01T13:24:09Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Soybean Allergens Meat products PCR |
| datacite.titles.title.fl_str_mv | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Amaral, Joana S. Soares, Sónia Mafra, Isabel Oliveira, Beatriz |
| dc.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-06-01T13:24:09Z |
| dc.date.embargoed.fl_str_mv | 2011-06-01T13:24:09Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/4866 |
| dc.language.none.fl_str_mv | por |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Soybean Allergens Meat products PCR |
| dc.title.fl_str_mv | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | In this work, a polymerase chain reaction (PCR) technique was used for soybean detection in processed meat products. The methodology was optimized using reference binary samples with different known percentages of pork meat and soybean protein, prepared in the laboratory. To evaluate the effect of thermal treatment, binary mixtures were submitted to thermal treatment at 121ºC for 5 min. The species-specific PCR targeting the lectin gene allowed the detection limit of 0.01 ng of soybean DNA in raw materials. The PCR method was successfully applied on 18 commercial Frankfurt sausages. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | conferencePaper |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/1ebfac42-542e-4dc4-a9a7-e9bb0b774486/download |
| id | ipb_3ff0fcf52d2d0dc623cc707b60ea4280 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/4866 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | por |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/4866 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Amaral, Joana S. Amaral, Joana S. https://www.ciencia-id.pt/5319-7DE8-BEDA 5319-7DE8-BEDA http://orcid.org/0000-0002-3648-7303 0000-0002-3648-7303 Soares, Sónia Mafra, Isabel Oliveira, Beatriz |
| publishDate | 2009 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | porporIn this work, a polymerase chain reaction (PCR) technique was used for soybean detection in processed meat products. The methodology was optimized using reference binary samples with different known percentages of pork meat and soybean protein, prepared in the laboratory. To evaluate the effect of thermal treatment, binary mixtures were submitted to thermal treatment at 121ºC for 5 min. The species-specific PCR targeting the lectin gene allowed the detection limit of 0.01 ng of soybean DNA in raw materials. The PCR method was successfully applied on 18 commercial Frankfurt sausages.application/pdfapplication/pdfporPolymerase chain reaction for the detection of allergens: the case of soybean in processed meat productsPersonalAmaral, Joana S.DSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdDSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdAmaralJoana S.Ciência IDhttps://www.ciencia-id.pt5319-7DE8-BEDAORCIDhttp://orcid.org0000-0002-3648-7303Soares, SóniaMafra, IsabelOliveira, BeatrizHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2011-06-01T13:24:09Z20092009-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/4866http://purl.org/coar/access_right/c_16ecrestricted accessSoybeanAllergensMeat productsPCR197957 bytes407939 bytesother research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/1ebfac42-542e-4dc4-a9a7-e9bb0b774486/downloadhttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/ab6aa489-797e-4961-ac9d-c1f40ec2bab6/downloadActas do 9º Encontro de Química dos Alimentos, CD-ROMAngra do Heroísmo |
| spellingShingle | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products Amaral, Joana S. Soybean Allergens Meat products PCR |
| status | SINGLETON |
| subject.fl_str_mv | Soybean Allergens Meat products PCR |
| title | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| title_full | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| title_fullStr | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| title_full_unstemmed | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| title_short | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| title_sort | Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products |
| topic | Soybean Allergens Meat products PCR |
| topic_facet | Soybean Allergens Meat products PCR |
| url | http://hdl.handle.net/10198/4866 |
| visible | 1 |