Publicação
In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network
| Resumo: | Microvascular networks are not simple straight microchannels but rather complex geometries composed by successive asymmetric divergent and convergent bifurcations. Despite the extensive research work in this field, still lack of knowledge about the blood flow behavior in microvascular networks. The current study applies the most current advanced visualization and microfabrication techniques to provide further insights into to the blood flow in network geometries. Hence, by using a high-speed video microscopy system, blood flow measurements and visualizations of the cell-free layer (CFL) were performed along a microchannel network composed by several divergent and convergent bifurcations. The inlet flow rate was kept constant whereas the hematocrit (Hct) and the depth of the geometry was changed in order to evaluate their effects into the CFL thickness. The results, show clearly that the Hct has a significant impact on the CFL thickness whereas the effect of reducing the depth did not contribute to a noticeable change on the CFL. In addition, the in vitro blood flow results reported here provide for the first time that in microfluidic devices having several asymmetric confluences it is likely to have the formation of several CFLs not only around the walls but also in middle of the main channels just downstream of the last confluence apex. Although, to best of our knowledge there is no evidence that this kind of flow phenomenon also happens in vivo, we believe that for microvascular networks with similar geometries and under similar flow conditions tested in this work, this kind of phenomenon may also happen in vivo. Furthermore, the results from this study could be extremely helpful to validate current numerical microvascular network models and to develop more realistic multiphase numerical models of blood flow in microcirculation. |
|---|---|
| Autores principais: | Bento, David |
| Outros Autores: | Fernandes, Carla S.; Miranda, João Mário; Lima, Rui A. |
| Assunto: | Microchannel network Cell-free layer Blood flow Microfluidics Red blood cells |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172679248773120 |
|---|---|
| author | Bento, David |
| author2 | Fernandes, Carla S. Miranda, João Mário Lima, Rui A. |
| author2_role | author author author |
| author_facet | Bento, David Fernandes, Carla S. Miranda, João Mário Lima, Rui A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Bento, David\"},{\"Person.name\":\"Fernandes, Carla S.\",\"Person.identifier.orcid\":\"0000-0002-3138-7493\"},{\"Person.name\":\"Miranda, João Mário\"},{\"Person.name\":\"Lima, Rui A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Bento, David Fernandes, Carla S. Miranda, João Mário Lima, Rui A. |
| datacite.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-03-20T12:22:13Z |
| datacite.date.embargoed.fl_str_mv | 2020-03-20T12:22:13Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Microchannel network Cell-free layer Blood flow Microfluidics Red blood cells |
| datacite.titles.title.fl_str_mv | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Bento, David Fernandes, Carla S. Miranda, João Mário Lima, Rui A. |
| dc.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-03-20T12:22:13Z |
| dc.date.embargoed.fl_str_mv | 2020-03-20T12:22:13Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/21073 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Microchannel network Cell-free layer Blood flow Microfluidics Red blood cells |
| dc.title.fl_str_mv | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Microvascular networks are not simple straight microchannels but rather complex geometries composed by successive asymmetric divergent and convergent bifurcations. Despite the extensive research work in this field, still lack of knowledge about the blood flow behavior in microvascular networks. The current study applies the most current advanced visualization and microfabrication techniques to provide further insights into to the blood flow in network geometries. Hence, by using a high-speed video microscopy system, blood flow measurements and visualizations of the cell-free layer (CFL) were performed along a microchannel network composed by several divergent and convergent bifurcations. The inlet flow rate was kept constant whereas the hematocrit (Hct) and the depth of the geometry was changed in order to evaluate their effects into the CFL thickness. The results, show clearly that the Hct has a significant impact on the CFL thickness whereas the effect of reducing the depth did not contribute to a noticeable change on the CFL. In addition, the in vitro blood flow results reported here provide for the first time that in microfluidic devices having several asymmetric confluences it is likely to have the formation of several CFLs not only around the walls but also in middle of the main channels just downstream of the last confluence apex. Although, to best of our knowledge there is no evidence that this kind of flow phenomenon also happens in vivo, we believe that for microvascular networks with similar geometries and under similar flow conditions tested in this work, this kind of phenomenon may also happen in vivo. Furthermore, the results from this study could be extremely helpful to validate current numerical microvascular network models and to develop more realistic multiphase numerical models of blood flow in microcirculation. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/b68ef575-8ac7-43de-bb22-4ad87a138221/download |
| id | ipb_44dfcbfe12bd3452b64d5fa17d81137a |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/21073 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/21073 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Bento, David Fernandes, Carla S. Fernandes, Carla S. https://www.ciencia-id.pt/F319-3D67-9DB7 F319-3D67-9DB7 http://orcid.org/0000-0002-3138-7493 0000-0002-3138-7493 Miranda, João Mário Lima, Rui A. |
| publishDate | 2019 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engpt_PTMicrovascular networks are not simple straight microchannels but rather complex geometries composed by successive asymmetric divergent and convergent bifurcations. Despite the extensive research work in this field, still lack of knowledge about the blood flow behavior in microvascular networks. The current study applies the most current advanced visualization and microfabrication techniques to provide further insights into to the blood flow in network geometries. Hence, by using a high-speed video microscopy system, blood flow measurements and visualizations of the cell-free layer (CFL) were performed along a microchannel network composed by several divergent and convergent bifurcations. The inlet flow rate was kept constant whereas the hematocrit (Hct) and the depth of the geometry was changed in order to evaluate their effects into the CFL thickness. The results, show clearly that the Hct has a significant impact on the CFL thickness whereas the effect of reducing the depth did not contribute to a noticeable change on the CFL. In addition, the in vitro blood flow results reported here provide for the first time that in microfluidic devices having several asymmetric confluences it is likely to have the formation of several CFLs not only around the walls but also in middle of the main channels just downstream of the last confluence apex. Although, to best of our knowledge there is no evidence that this kind of flow phenomenon also happens in vivo, we believe that for microvascular networks with similar geometries and under similar flow conditions tested in this work, this kind of phenomenon may also happen in vivo. Furthermore, the results from this study could be extremely helpful to validate current numerical microvascular network models and to develop more realistic multiphase numerical models of blood flow in microcirculation.application/pdfpt_PTIn vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel networkBento, DavidPersonalFernandes, Carla S.DSpacehttp://dspace.org/items/8f9cf139-cfef-4025-ba87-56425618bc6eDSpacehttp://dspace.org/items/8f9cf139-cfef-4025-ba87-56425618bc6eFernandesCarla S.Ciência IDhttps://www.ciencia-id.ptF319-3D67-9DB7ORCIDhttp://orcid.org0000-0002-3138-7493Researcher IDhttps://www.researcherid.comA-4269-2015Scopus Author IDhttps://www.scopus.com8253740700Miranda, João MárioLima, Rui A.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0894-1777DOIIsPartOf10.1016/j.expthermflusci.2019.1098472020-03-20T12:22:13Z20192019-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/21073http://purl.org/coar/access_right/c_abf2open accessMicrochannel networkCell-free layerBlood flowMicrofluidicsRed blood cells5206428 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2019http://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/b68ef575-8ac7-43de-bb22-4ad87a138221/downloadExperimental Thermal and Fluid Science109109847 |
| spellingShingle | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network Bento, David Microchannel network Cell-free layer Blood flow Microfluidics Red blood cells |
| status | SINGLETON |
| subject.fl_str_mv | Microchannel network Cell-free layer Blood flow Microfluidics Red blood cells |
| title | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| title_full | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| title_fullStr | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| title_full_unstemmed | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| title_short | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| title_sort | In vitro blood flow visualizations and cell-free layer (CFL) measurements in a microchannel network |
| topic | Microchannel network Cell-free layer Blood flow Microfluidics Red blood cells |
| topic_facet | Microchannel network Cell-free layer Blood flow Microfluidics Red blood cells |
| url | http://hdl.handle.net/10198/21073 |
| visible | 1 |