Publicação
Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth
| Resumo: | Thymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH•), hydroxyl (•OH), and nitric oxide (•NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for •NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 = 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents. |
|---|---|
| Autores principais: | Haile, Tesfay |
| Outros Autores: | Cardoso, Susana M.; Raphaelli, Chirle de Oliveira; Pereira, Olívia R.; Pereira, Elisa dos Santos; Vizzotto, Márcia; Nora, Leonardo; Asfaw, Adissu Alemayehu; Periasamy, Gomathi; Karim, Aman |
| Assunto: | Antioxidant activity Essential oil Thyme-decoction Alpha-amylase, Thymus serrulatus Alphaglucosidase Phenolic compounds profile Antihyperglycemic activity |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173196432670720 |
|---|---|
| author | Haile, Tesfay |
| author2 | Cardoso, Susana M. Raphaelli, Chirle de Oliveira Pereira, Olívia R. Pereira, Elisa dos Santos Vizzotto, Márcia Nora, Leonardo Asfaw, Adissu Alemayehu Periasamy, Gomathi Karim, Aman |
| author2_role | author author author author author author author author author |
| author_facet | Haile, Tesfay Cardoso, Susana M. Raphaelli, Chirle de Oliveira Pereira, Olívia R. Pereira, Elisa dos Santos Vizzotto, Márcia Nora, Leonardo Asfaw, Adissu Alemayehu Periasamy, Gomathi Karim, Aman |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Haile, Tesfay\"},{\"Person.name\":\"Cardoso, Susana M.\"},{\"Person.name\":\"Raphaelli, Chirle de Oliveira\"},{\"Person.name\":\"Pereira, Olívia R.\",\"Person.identifier.orcid\":\"0000-0002-6275-3134\"},{\"Person.name\":\"Pereira, Elisa dos Santos\"},{\"Person.name\":\"Vizzotto, Márcia\"},{\"Person.name\":\"Nora, Leonardo\"},{\"Person.name\":\"Asfaw, Adissu Alemayehu\"},{\"Person.name\":\"Periasamy, Gomathi\"},{\"Person.name\":\"Karim, Aman\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Haile, Tesfay Cardoso, Susana M. Raphaelli, Chirle de Oliveira Pereira, Olívia R. Pereira, Elisa dos Santos Vizzotto, Márcia Nora, Leonardo Asfaw, Adissu Alemayehu Periasamy, Gomathi Karim, Aman |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-06-15T14:09:03Z |
| datacite.date.embargoed.fl_str_mv | 2021-06-15T14:09:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Antioxidant activity Essential oil Thyme-decoction Alpha-amylase, Thymus serrulatus Alphaglucosidase Phenolic compounds profile Antihyperglycemic activity |
| datacite.titles.title.fl_str_mv | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Haile, Tesfay Cardoso, Susana M. Raphaelli, Chirle de Oliveira Pereira, Olívia R. Pereira, Elisa dos Santos Vizzotto, Márcia Nora, Leonardo Asfaw, Adissu Alemayehu Periasamy, Gomathi Karim, Aman |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-06-15T14:09:03Z |
| dc.date.embargoed.fl_str_mv | 2021-06-15T14:09:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/23656 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Frontiers |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Antioxidant activity Essential oil Thyme-decoction Alpha-amylase, Thymus serrulatus Alphaglucosidase Phenolic compounds profile Antihyperglycemic activity |
| dc.title.fl_str_mv | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Thymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH•), hydroxyl (•OH), and nitric oxide (•NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for •NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 = 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/b4cc3e66-d220-4e2e-97a5-aa2469973ad3/download |
| id | ipb_455863ce142519c3a1bbdade3dcfa2ef |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/23656 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/23656 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Haile, Tesfay Cardoso, Susana M. Raphaelli, Chirle de Oliveira Pereira, Olívia R. Pereira, Olívia R. https://www.ciencia-id.pt/B312-AF21-D2FA B312-AF21-D2FA http://orcid.org/0000-0002-6275-3134 0000-0002-6275-3134 Pereira, Elisa dos Santos Vizzotto, Márcia Nora, Leonardo Asfaw, Adissu Alemayehu Periasamy, Gomathi Karim, Aman |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Frontiers |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engFrontierspt_PTThymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH•), hydroxyl (•OH), and nitric oxide (•NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for •NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 = 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents.application/pdfpt_PTChemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex BenthHaile, TesfayCardoso, Susana M.Raphaelli, Chirle de OliveiraPersonalPereira, Olívia R.DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453PereiraOlívia R.Ciência IDhttps://www.ciencia-id.ptB312-AF21-D2FAORCIDhttp://orcid.org0000-0002-6275-3134Researcher IDhttps://www.researcherid.comH-1049-2014Scopus Author IDhttps://www.scopus.com52264387000Pereira, Elisa dos SantosVizzotto, MárciaNora, LeonardoAsfaw, Adissu AlemayehuPeriasamy, GomathiKarim, AmanHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf1663-9812DOIIsPartOf10.3389/fphar.2021.6215362021-06-15T14:09:03Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/23656http://purl.org/coar/access_right/c_abf2open accessAntioxidant activityEssential oilThyme-decoctionAlpha-amylase,Thymus serrulatusAlphaglucosidasePhenolic compounds profileAntihyperglycemic activity1083947 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2021http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/b4cc3e66-d220-4e2e-97a5-aa2469973ad3/downloadFrontiers in Pharmacology12621536 |
| spellingShingle | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth Haile, Tesfay Antioxidant activity Essential oil Thyme-decoction Alpha-amylase, Thymus serrulatus Alphaglucosidase Phenolic compounds profile Antihyperglycemic activity |
| status | SINGLETON |
| subject.fl_str_mv | Antioxidant activity Essential oil Thyme-decoction Alpha-amylase, Thymus serrulatus Alphaglucosidase Phenolic compounds profile Antihyperglycemic activity |
| title | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| title_full | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| title_fullStr | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| title_full_unstemmed | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| title_short | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| title_sort | Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth |
| topic | Antioxidant activity Essential oil Thyme-decoction Alpha-amylase, Thymus serrulatus Alphaglucosidase Phenolic compounds profile Antihyperglycemic activity |
| topic_facet | Antioxidant activity Essential oil Thyme-decoction Alpha-amylase, Thymus serrulatus Alphaglucosidase Phenolic compounds profile Antihyperglycemic activity |
| url | http://hdl.handle.net/10198/23656 |
| visible | 1 |