Publicação
SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS
| Resumo: | Single nucleotide polymorphisms (SNPs) have a great potential in genetic identification and introgression studies of honey bees (Apis mellifera). To achieve SNPs full power in genetic analysis, they must be screened in a rapid, accurate and cost-effective manner. Matrix-assisted laser desorption-ionization–time-of-flight (MALDI–TOF) mass spectrometry is a promising tool for the high-throughput screening of SNPs at an affordable cost in the post-genome sequencing era. In this study, a reduced SNP panel has been validated for Iberian honey bees (A. m. iberiensis) and a pooling strategy is presented for allele frequencies determination by using MALDI-TOF technology. The reduced SNP panel contains 127 most ancestry-informative loci design in the dark honey bee (A. m. mellifera). We demonstrate the utility of this methodology in samples of Iberian honey bees (A. m. iberiensis) selected for its pathogen tolerance and in genomic DNA pools. The described method reduces costs and time and enables genotyping of up to thousands of honey bee samples by taking advantage of the high-throughput MALDI-TOF technology. |
|---|---|
| Autores principais: | Muñoz, Irene |
| Outros Autores: | Martín-Hernández, Raquel; Higes, Mariano; De la Rúa, Pilar; Pinto, M. Alice |
| Assunto: | SNP assays MALDI-TOF genotyping Honeybee Apis mellifera |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851013657591808 |
|---|---|
| author | Muñoz, Irene |
| author2 | Martín-Hernández, Raquel Higes, Mariano De la Rúa, Pilar Pinto, M. Alice |
| author2_role | author author author author |
| author_facet | Muñoz, Irene Martín-Hernández, Raquel Higes, Mariano De la Rúa, Pilar Pinto, M. Alice |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Muñoz, Irene\"},{\"Person.name\":\"Martín-Hernández, Raquel\"},{\"Person.name\":\"Higes, Mariano\"},{\"Person.name\":\"De la Rúa, Pilar\"},{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Muñoz, Irene Martín-Hernández, Raquel Higes, Mariano De la Rúa, Pilar Pinto, M. Alice |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-02-15T14:24:59Z |
| datacite.date.embargoed.fl_str_mv | 2018-02-15T14:24:59Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | SNP assays MALDI-TOF genotyping Honeybee Apis mellifera |
| datacite.titles.title.fl_str_mv | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Muñoz, Irene Martín-Hernández, Raquel Higes, Mariano De la Rúa, Pilar Pinto, M. Alice |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-02-15T14:24:59Z |
| dc.date.embargoed.fl_str_mv | 2018-02-15T14:24:59Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/15768 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | SNP assays MALDI-TOF genotyping Honeybee Apis mellifera |
| dc.title.fl_str_mv | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Single nucleotide polymorphisms (SNPs) have a great potential in genetic identification and introgression studies of honey bees (Apis mellifera). To achieve SNPs full power in genetic analysis, they must be screened in a rapid, accurate and cost-effective manner. Matrix-assisted laser desorption-ionization–time-of-flight (MALDI–TOF) mass spectrometry is a promising tool for the high-throughput screening of SNPs at an affordable cost in the post-genome sequencing era. In this study, a reduced SNP panel has been validated for Iberian honey bees (A. m. iberiensis) and a pooling strategy is presented for allele frequencies determination by using MALDI-TOF technology. The reduced SNP panel contains 127 most ancestry-informative loci design in the dark honey bee (A. m. mellifera). We demonstrate the utility of this methodology in samples of Iberian honey bees (A. m. iberiensis) selected for its pathogen tolerance and in genomic DNA pools. The described method reduces costs and time and enables genotyping of up to thousands of honey bee samples by taking advantage of the high-throughput MALDI-TOF technology. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/45fea66d-1a3e-4470-84a2-176bcc941f34/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/15768 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/15768 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Muñoz, Irene Martín-Hernández, Raquel Higes, Mariano De la Rúa, Pilar Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 |
| publishDate | 2017 |
| reponame_str | Biblioteca Digital do IPB |
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| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engpt_PTSingle nucleotide polymorphisms (SNPs) have a great potential in genetic identification and introgression studies of honey bees (Apis mellifera). To achieve SNPs full power in genetic analysis, they must be screened in a rapid, accurate and cost-effective manner. Matrix-assisted laser desorption-ionization–time-of-flight (MALDI–TOF) mass spectrometry is a promising tool for the high-throughput screening of SNPs at an affordable cost in the post-genome sequencing era. In this study, a reduced SNP panel has been validated for Iberian honey bees (A. m. iberiensis) and a pooling strategy is presented for allele frequencies determination by using MALDI-TOF technology. The reduced SNP panel contains 127 most ancestry-informative loci design in the dark honey bee (A. m. mellifera). We demonstrate the utility of this methodology in samples of Iberian honey bees (A. m. iberiensis) selected for its pathogen tolerance and in genomic DNA pools. The described method reduces costs and time and enables genotyping of up to thousands of honey bee samples by taking advantage of the high-throughput MALDI-TOF technology.application/pdfpt_PTSNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MSMuñoz, IreneMartín-Hernández, RaquelHiges, MarianoDe la Rúa, PilarPersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2018-02-15T14:24:59Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/15768http://purl.org/coar/access_right/c_abf2open accessSNP assaysMALDI-TOF genotypingHoneybeeApis mellifera1018796 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2017http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/45fea66d-1a3e-4470-84a2-176bcc941f34/downloadXVI Congress of the European Society for Evolutionary BiologyGroningen, The Netherlands |
| spellingShingle | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS Muñoz, Irene SNP assays MALDI-TOF genotyping Honeybee Apis mellifera |
| subject.fl_str_mv | SNP assays MALDI-TOF genotyping Honeybee Apis mellifera |
| title | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| title_full | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| title_fullStr | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| title_full_unstemmed | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| title_short | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| title_sort | SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS |
| topic | SNP assays MALDI-TOF genotyping Honeybee Apis mellifera |
| topic_facet | SNP assays MALDI-TOF genotyping Honeybee Apis mellifera |
| url | http://hdl.handle.net/10198/15768 |
| visible | 1 |