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Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts

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Resumo:Walnut green husk is an agro-forest waste generated in the walnut (Juglans regia L.) harvest that could be valued as a source of natural compounds with antioxidant and antimicrobial properties. At this respect, the effect of the solvent (water, methanol, ethanol and 50% aqueous solutions of methanol and ethanol) on the extraction yields and extracts bioactive properties was analysed. Total phenols content of the extracts was determined by the Folin–Ciocalteau method. Extract antioxidant activity was evaluated using the reducing power assay and by the ability of the extracts to scavenge the DPPH radical. The scavenging effect of the aqueous extracts on the nitric oxide radical was also evaluated. The highest extraction yield was achieved with water (44.11%) and high bioactive potential was shown by the samples extracted with water/ethanol (1:1) (84.46 mg GAE/g extract; EC50 = 0.95 mg/mL for reducing power and EC50 = 0.33 mg/mL for DPPH assay). All the antioxidant properties analysed showed a concentrationdependent activity. The antimicrobial activity of the aqueous extracts was assessed and showed ability to inhibit the growth of Gram positive bacteria. The results obtained demonstrated the potential of the walnut green husk as an economical source of antioxidant and antimicrobial agents.
Autores principais:Fernández-Agulló, Adela
Outros Autores:Pereira, Etelvina; Freire, M. Sonia; Valentão, Patrícia; Andrade, Paula B.; González-Álvarez, Julia; Pereira, J.A.
Assunto:Walnut green husk Solvent extraction Total phenols content Antioxidant activity Antimicrobial activity
Ano:2013
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Fernández-Agulló, Adela
author2 Pereira, Etelvina
Freire, M. Sonia
Valentão, Patrícia
Andrade, Paula B.
González-Álvarez, Julia
Pereira, J.A.
author2_role author
author
author
author
author
author
author_facet Fernández-Agulló, Adela
Pereira, Etelvina
Freire, M. Sonia
Valentão, Patrícia
Andrade, Paula B.
González-Álvarez, Julia
Pereira, J.A.
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_str [{\"Person.name\":\"Fernández-Agulló, Adela\"},{\"Person.name\":\"Pereira, Etelvina\"},{\"Person.name\":\"Freire, M. Sonia\"},{\"Person.name\":\"Valentão, Patrícia\"},{\"Person.name\":\"Andrade, Paula B.\"},{\"Person.name\":\"González-Álvarez, Julia\"},{\"Person.name\":\"Pereira, J.A.\",\"Person.identifier.orcid\":\"0000-0002-2260-0600\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Fernández-Agulló, Adela
Pereira, Etelvina
Freire, M. Sonia
Valentão, Patrícia
Andrade, Paula B.
González-Álvarez, Julia
Pereira, J.A.
datacite.date.Accepted.fl_str_mv 2013-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2016-12-21T09:51:52Z
datacite.date.embargoed.fl_str_mv 2016-12-21T09:51:52Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv Walnut green husk
Solvent extraction
Total phenols content
Antioxidant activity
Antimicrobial activity
datacite.titles.title.fl_str_mv Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Fernández-Agulló, Adela
Pereira, Etelvina
Freire, M. Sonia
Valentão, Patrícia
Andrade, Paula B.
González-Álvarez, Julia
Pereira, J.A.
dc.date.Accepted.fl_str_mv 2013-01-01T00:00:00Z
dc.date.available.fl_str_mv 2016-12-21T09:51:52Z
dc.date.embargoed.fl_str_mv 2016-12-21T09:51:52Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/13628
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Elsevier
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv Walnut green husk
Solvent extraction
Total phenols content
Antioxidant activity
Antimicrobial activity
dc.title.fl_str_mv Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Walnut green husk is an agro-forest waste generated in the walnut (Juglans regia L.) harvest that could be valued as a source of natural compounds with antioxidant and antimicrobial properties. At this respect, the effect of the solvent (water, methanol, ethanol and 50% aqueous solutions of methanol and ethanol) on the extraction yields and extracts bioactive properties was analysed. Total phenols content of the extracts was determined by the Folin–Ciocalteau method. Extract antioxidant activity was evaluated using the reducing power assay and by the ability of the extracts to scavenge the DPPH radical. The scavenging effect of the aqueous extracts on the nitric oxide radical was also evaluated. The highest extraction yield was achieved with water (44.11%) and high bioactive potential was shown by the samples extracted with water/ethanol (1:1) (84.46 mg GAE/g extract; EC50 = 0.95 mg/mL for reducing power and EC50 = 0.33 mg/mL for DPPH assay). All the antioxidant properties analysed showed a concentrationdependent activity. The antimicrobial activity of the aqueous extracts was assessed and showed ability to inhibit the growth of Gram positive bacteria. The results obtained demonstrated the potential of the walnut green husk as an economical source of antioxidant and antimicrobial agents.
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eu_rights_str_mv restrictedAccess
format article
fulltext.url.fl_str_mv https://bibliotecadigital.ipb.pt/bitstreams/4b547a3a-b062-49fc-be77-549361dc85c1/download
id ipb_4f54f3274e8e0cd464a68d7efbd4e868
identifier.url.fl_str_mv http://hdl.handle.net/10198/13628
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institution Instituto Politécnico de Bragança
instname_str Instituto Politécnico de Bragança
language eng
network_acronym_str ipb
network_name_str Biblioteca Digital do IPB
oai_identifier_str oai:bibliotecadigital.ipb.pt:10198/13628
organization_str_mv urn:organizationAcronym:ipb
person_str_mv Fernández-Agulló, Adela
Pereira, Etelvina
Freire, M. Sonia
Valentão, Patrícia
Andrade, Paula B.
González-Álvarez, Julia
Pereira, J.A.
Pereira, J.A.
https://www.ciencia-id.pt/611F-80B2-A7C1
611F-80B2-A7C1
http://orcid.org/0000-0002-2260-0600
0000-0002-2260-0600
publishDate 2013
publisher.none.fl_str_mv Elsevier
reponame_str Biblioteca Digital do IPB
repository_id_str urn:repositoryAcronym:ipb
service_str_mv urn:repositoryAcronym:ipb
spelling engElsevierpt_PTWalnut green husk is an agro-forest waste generated in the walnut (Juglans regia L.) harvest that could be valued as a source of natural compounds with antioxidant and antimicrobial properties. At this respect, the effect of the solvent (water, methanol, ethanol and 50% aqueous solutions of methanol and ethanol) on the extraction yields and extracts bioactive properties was analysed. Total phenols content of the extracts was determined by the Folin–Ciocalteau method. Extract antioxidant activity was evaluated using the reducing power assay and by the ability of the extracts to scavenge the DPPH radical. The scavenging effect of the aqueous extracts on the nitric oxide radical was also evaluated. The highest extraction yield was achieved with water (44.11%) and high bioactive potential was shown by the samples extracted with water/ethanol (1:1) (84.46 mg GAE/g extract; EC50 = 0.95 mg/mL for reducing power and EC50 = 0.33 mg/mL for DPPH assay). All the antioxidant properties analysed showed a concentrationdependent activity. The antimicrobial activity of the aqueous extracts was assessed and showed ability to inhibit the growth of Gram positive bacteria. The results obtained demonstrated the potential of the walnut green husk as an economical source of antioxidant and antimicrobial agents.application/pdfpt_PTInfluence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extractsFernández-Agulló, AdelaPereira, EtelvinaFreire, M. SoniaValentão, PatríciaAndrade, Paula B.González-Álvarez, JuliaPersonalPereira, J.A.DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857PereiraJosé AlbertoCiência IDhttps://www.ciencia-id.pt611F-80B2-A7C1ORCIDhttp://orcid.org0000-0002-2260-0600Researcher IDhttps://www.researcherid.comL-6798-2014Scopus Author IDhttps://www.scopus.com57204366348HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0926-6690DOIIsPartOf10.1016/j.indcrop.2012.05.0212016-12-21T09:51:52Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/13628http://purl.org/coar/access_right/c_16ecrestricted accessWalnut green huskSolvent extractionTotal phenols contentAntioxidant activityAntimicrobial activity395580 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2013http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/4b547a3a-b062-49fc-be77-549361dc85c1/downloadIndustrial Crops and Products42126132
spellingShingle Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
Fernández-Agulló, Adela
Walnut green husk
Solvent extraction
Total phenols content
Antioxidant activity
Antimicrobial activity
subject.fl_str_mv Walnut green husk
Solvent extraction
Total phenols content
Antioxidant activity
Antimicrobial activity
title Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
title_full Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
title_fullStr Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
title_full_unstemmed Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
title_short Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
title_sort Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts
topic Walnut green husk
Solvent extraction
Total phenols content
Antioxidant activity
Antimicrobial activity
topic_facet Walnut green husk
Solvent extraction
Total phenols content
Antioxidant activity
Antimicrobial activity
url http://hdl.handle.net/10198/13628
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