Publicação
Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors
| Resumo: | Xylella fastidiosa is a plant pathogen transmitted by insect vectors that cause high losses in a variety of agriculturally important crops. In Europe, an outbreak of X. fastidiosa was first detected in 2013 in olive trees in Southern Italy, being Philaenus spumarius (L.) identified as the vector involved in this outbreak. In this work, as a first approach, we explored the endophytic microbial community inhabiting plants in order to design new approaches to manage the X. fastidiosa in the olive tree and its insect vector. Nymphs of P. spumarius feeds commonly on stems of Coleostephus myconis (L.), which is a common weed found in olive groves. A better understand of the feeding effect on the fungal community residing in this weed and of the role of these fungi in feeding choice could assist in the manipulation of P. spumarius populations. For this, the endophytic fungal community of stems, leaves and inflorescences of C. myconis with three levels of P. spumarius infestation was studied by cultural dependent method. Feeding was showed to affect the fungal communities in the stem and leaf but not the ones in the inflorescence. A set of fungi was identified to be associated to either infested or non-infested plants, suggesting playing a key role on the feeding process. As a second approach, it was observed, in the outbreak, the presence of olive cultivars resistant (cv. FS17) or susceptible (cv. Kalamata) to X. fastidiosa. The study of the xylem microbiome of these two cultivars, was performed through Illumina amplicon sequencing in order to determine whether susceptibilities differences are linked to its core microbiome. Overall, the core microbiome was dominated by members of the Proteobacteria (81% of the total bacteria reads) and Ascomycota (98% of the total fungal reads). In non-infected trees, the core microbiome was found to differ between susceptible and resistant cultivars. The presence of X. fastidiosa showed to induce greater variation in microbial composition and diversity of the susceptible cultivar than of the resistant cultivar. Specific fungal/bacterial signatures were detected to either the presence or the absence of X. fastidiosa in the xylem vessels, suggesting an important role of these microorganisms in pathogen establishment/development. Our study provides microbial candidates to further evaluate their role in the management of P. spumarius and X. fastidiosa. |
|---|---|
| Autores principais: | Cameirão, Cristina |
| Assunto: | Xylella fastidiosa Philaenus spumarius Endophytes Host plant Xylem Microbiome Biocontrol |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172952761434112 |
|---|---|
| author | Cameirão, Cristina |
| author_facet | Cameirão, Cristina |
| author_role | author |
| contributor_name_str_mv | Baptista, Paula Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cameirão, Cristina\",\"Person.identifier.orcid\":\"0000-0001-5745-9822\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Baptista, Paula Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Cameirão, Cristina |
| datacite.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-01-15T15:08:19Z |
| datacite.date.embargoed.fl_str_mv | 2019-01-15T15:08:19Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Xylella fastidiosa Philaenus spumarius Endophytes Host plant Xylem Microbiome Biocontrol |
| datacite.titles.title.fl_str_mv | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| dc.contributor.none.fl_str_mv | Baptista, Paula Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Cameirão, Cristina |
| dc.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-01-15T15:08:19Z |
| dc.date.embargoed.fl_str_mv | 2019-01-15T15:08:19Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/18471 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Xylella fastidiosa Philaenus spumarius Endophytes Host plant Xylem Microbiome Biocontrol |
| dc.title.fl_str_mv | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | Xylella fastidiosa is a plant pathogen transmitted by insect vectors that cause high losses in a variety of agriculturally important crops. In Europe, an outbreak of X. fastidiosa was first detected in 2013 in olive trees in Southern Italy, being Philaenus spumarius (L.) identified as the vector involved in this outbreak. In this work, as a first approach, we explored the endophytic microbial community inhabiting plants in order to design new approaches to manage the X. fastidiosa in the olive tree and its insect vector. Nymphs of P. spumarius feeds commonly on stems of Coleostephus myconis (L.), which is a common weed found in olive groves. A better understand of the feeding effect on the fungal community residing in this weed and of the role of these fungi in feeding choice could assist in the manipulation of P. spumarius populations. For this, the endophytic fungal community of stems, leaves and inflorescences of C. myconis with three levels of P. spumarius infestation was studied by cultural dependent method. Feeding was showed to affect the fungal communities in the stem and leaf but not the ones in the inflorescence. A set of fungi was identified to be associated to either infested or non-infested plants, suggesting playing a key role on the feeding process. As a second approach, it was observed, in the outbreak, the presence of olive cultivars resistant (cv. FS17) or susceptible (cv. Kalamata) to X. fastidiosa. The study of the xylem microbiome of these two cultivars, was performed through Illumina amplicon sequencing in order to determine whether susceptibilities differences are linked to its core microbiome. Overall, the core microbiome was dominated by members of the Proteobacteria (81% of the total bacteria reads) and Ascomycota (98% of the total fungal reads). In non-infected trees, the core microbiome was found to differ between susceptible and resistant cultivars. The presence of X. fastidiosa showed to induce greater variation in microbial composition and diversity of the susceptible cultivar than of the resistant cultivar. Specific fungal/bacterial signatures were detected to either the presence or the absence of X. fastidiosa in the xylem vessels, suggesting an important role of these microorganisms in pathogen establishment/development. Our study provides microbial candidates to further evaluate their role in the management of P. spumarius and X. fastidiosa. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | masterThesis |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/ca11e76b-4ac1-4dee-8810-09bd3dfd3210/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 3599-PPCDT |
| id | ipb_50db9396ed254435cb652c9a1fd1f736 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/18471 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/18471 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Cameirão, Cristina Cameirão, Cristina https://www.ciencia-id.pt/9511-984B-7350 9511-984B-7350 http://orcid.org/0000-0001-5745-9822 0000-0001-5745-9822 |
| publishDate | 2019 |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engpt_PTXylella fastidiosa is a plant pathogen transmitted by insect vectors that cause high losses in a variety of agriculturally important crops. In Europe, an outbreak of X. fastidiosa was first detected in 2013 in olive trees in Southern Italy, being Philaenus spumarius (L.) identified as the vector involved in this outbreak. In this work, as a first approach, we explored the endophytic microbial community inhabiting plants in order to design new approaches to manage the X. fastidiosa in the olive tree and its insect vector. Nymphs of P. spumarius feeds commonly on stems of Coleostephus myconis (L.), which is a common weed found in olive groves. A better understand of the feeding effect on the fungal community residing in this weed and of the role of these fungi in feeding choice could assist in the manipulation of P. spumarius populations. For this, the endophytic fungal community of stems, leaves and inflorescences of C. myconis with three levels of P. spumarius infestation was studied by cultural dependent method. Feeding was showed to affect the fungal communities in the stem and leaf but not the ones in the inflorescence. A set of fungi was identified to be associated to either infested or non-infested plants, suggesting playing a key role on the feeding process. As a second approach, it was observed, in the outbreak, the presence of olive cultivars resistant (cv. FS17) or susceptible (cv. Kalamata) to X. fastidiosa. The study of the xylem microbiome of these two cultivars, was performed through Illumina amplicon sequencing in order to determine whether susceptibilities differences are linked to its core microbiome. Overall, the core microbiome was dominated by members of the Proteobacteria (81% of the total bacteria reads) and Ascomycota (98% of the total fungal reads). In non-infected trees, the core microbiome was found to differ between susceptible and resistant cultivars. The presence of X. fastidiosa showed to induce greater variation in microbial composition and diversity of the susceptible cultivar than of the resistant cultivar. Specific fungal/bacterial signatures were detected to either the presence or the absence of X. fastidiosa in the xylem vessels, suggesting an important role of these microorganisms in pathogen establishment/development. Our study provides microbial candidates to further evaluate their role in the management of P. spumarius and X. fastidiosa.application/pdfpt_PTExploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectorsPersonalCameirão, CristinaDSpacehttp://dspace.org/items/c7e28508-95c5-4441-a2c3-91d69059016dDSpacehttp://dspace.org/items/c7e28508-95c5-4441-a2c3-91d69059016dCameirãoCristinaCiência IDhttps://www.ciencia-id.pt9511-984B-7350ORCIDhttp://orcid.org0000-0001-5745-9822Baptista, PaulaHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptURNurn:tid:2021398082019-01-15T15:08:19Z201920182019-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/18471http://purl.org/coar/access_right/c_16ecrestricted accessXylella fastidiosaPhilaenus spumariusEndophytesHost plantXylemMicrobiomeBiocontrol2877496 bytesFundação para a Ciência e a TecnologiaMultifunctional study of xylem-sap of Portuguese olive cultivars and its relation with susceptibility to infection by Xylella fastidiosa3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_bdccmaster thesis2019http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/ca11e76b-4ac1-4dee-8810-09bd3dfd3210/download |
| spellingShingle | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors Cameirão, Cristina Xylella fastidiosa Philaenus spumarius Endophytes Host plant Xylem Microbiome Biocontrol |
| status | SINGLETON |
| subject.fl_str_mv | Xylella fastidiosa Philaenus spumarius Endophytes Host plant Xylem Microbiome Biocontrol |
| title | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| title_full | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| title_fullStr | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| title_full_unstemmed | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| title_short | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| title_sort | Exploitation of plant microbiota in the development of new approaches to manage Xylella fastidiosa and its insect vectors |
| topic | Xylella fastidiosa Philaenus spumarius Endophytes Host plant Xylem Microbiome Biocontrol |
| topic_facet | Xylella fastidiosa Philaenus spumarius Endophytes Host plant Xylem Microbiome Biocontrol |
| url | http://hdl.handle.net/10198/18471 |
| visible | 1 |